Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10595 and BWI76_RS10600 are separated by 55 nucleotides BWI76_RS10600 and BWI76_RS10605 are separated by 71 nucleotides
BWI76_RS10595: BWI76_RS10595 - allantoate amidohydrolase, at 2,236,956 to 2,238,218
_RS10595
BWI76_RS10600: BWI76_RS10600 - pectate lyase, at 2,238,274 to 2,239,983
_RS10600
BWI76_RS10605: BWI76_RS10605 - oligogalacturonate porin, at 2,240,055 to 2,240,756
_RS10605
Position (kb)
2238
2239
2240 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2237.402 kb on + strand, within BWI76_RS10595 at 2237.518 kb on + strand, within BWI76_RS10595 at 2237.603 kb on - strand, within BWI76_RS10595 at 2237.660 kb on + strand, within BWI76_RS10595 at 2237.826 kb on + strand, within BWI76_RS10595 at 2238.015 kb on + strand, within BWI76_RS10595 at 2238.165 kb on - strand at 2238.252 kb on - strand at 2238.290 kb on + strand at 2238.298 kb on - strand at 2238.577 kb on + strand, within BWI76_RS10600 at 2238.697 kb on + strand, within BWI76_RS10600 at 2238.896 kb on + strand, within BWI76_RS10600 at 2238.901 kb on + strand, within BWI76_RS10600 at 2238.904 kb on - strand, within BWI76_RS10600 at 2238.933 kb on + strand, within BWI76_RS10600 at 2239.140 kb on + strand, within BWI76_RS10600 at 2239.218 kb on - strand, within BWI76_RS10600 at 2239.396 kb on + strand, within BWI76_RS10600 at 2239.495 kb on + strand, within BWI76_RS10600 at 2239.604 kb on + strand, within BWI76_RS10600 at 2239.611 kb on + strand, within BWI76_RS10600 at 2239.612 kb on - strand, within BWI76_RS10600 at 2239.710 kb on - strand, within BWI76_RS10600 at 2239.710 kb on - strand, within BWI76_RS10600 at 2239.747 kb on + strand, within BWI76_RS10600 at 2239.747 kb on - strand, within BWI76_RS10600 at 2239.763 kb on + strand, within BWI76_RS10600 at 2239.809 kb on + strand, within BWI76_RS10600 at 2239.817 kb on - strand at 2239.827 kb on + strand at 2239.830 kb on + strand at 2239.830 kb on + strand at 2239.837 kb on + strand at 2239.872 kb on - strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.887 kb on + strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.895 kb on - strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.897 kb on + strand at 2239.905 kb on - strand at 2239.905 kb on - strand at 2239.905 kb on - strand at 2239.905 kb on - strand at 2239.905 kb on - strand at 2239.905 kb on - strand at 2239.905 kb on - strand at 2239.905 kb on - strand at 2239.905 kb on - strand at 2239.905 kb on - strand at 2239.905 kb on - strand at 2239.905 kb on - strand at 2239.907 kb on + strand at 2239.912 kb on + strand at 2239.915 kb on - strand at 2239.920 kb on + strand at 2239.922 kb on - strand at 2239.937 kb on + strand at 2239.940 kb on - strand at 2239.952 kb on + strand at 2239.952 kb on + strand at 2239.952 kb on + strand at 2239.952 kb on + strand at 2239.952 kb on + strand at 2239.952 kb on + strand at 2239.960 kb on - strand at 2239.960 kb on - strand at 2239.960 kb on - strand at 2240.001 kb on + strand at 2240.016 kb on - strand at 2240.029 kb on + strand at 2240.051 kb on - strand at 2240.067 kb on + strand at 2240.086 kb on - strand at 2240.086 kb on - strand at 2240.088 kb on + strand at 2240.096 kb on - strand at 2240.096 kb on - strand at 2240.096 kb on - strand at 2240.096 kb on - strand at 2240.096 kb on - strand at 2240.104 kb on - strand at 2240.131 kb on - strand, within BWI76_RS10605 at 2240.182 kb on - strand, within BWI76_RS10605 at 2240.200 kb on - strand, within BWI76_RS10605 at 2240.291 kb on + strand, within BWI76_RS10605 at 2240.291 kb on + strand, within BWI76_RS10605 at 2240.299 kb on - strand, within BWI76_RS10605 at 2240.299 kb on - strand, within BWI76_RS10605 at 2240.301 kb on + strand, within BWI76_RS10605 at 2240.306 kb on + strand, within BWI76_RS10605 at 2240.306 kb on + strand, within BWI76_RS10605 at 2240.306 kb on + strand, within BWI76_RS10605 at 2240.306 kb on + strand, within BWI76_RS10605 at 2240.306 kb on + strand, within BWI76_RS10605 at 2240.306 kb on + strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.314 kb on - strand, within BWI76_RS10605 at 2240.328 kb on + strand, within BWI76_RS10605 at 2240.330 kb on + strand, within BWI76_RS10605 at 2240.330 kb on + strand, within BWI76_RS10605 at 2240.336 kb on - strand, within BWI76_RS10605 at 2240.336 kb on - strand, within BWI76_RS10605 at 2240.336 kb on - strand, within BWI76_RS10605 at 2240.336 kb on - strand, within BWI76_RS10605 at 2240.338 kb on - strand, within BWI76_RS10605 at 2240.338 kb on - strand, within BWI76_RS10605 at 2240.338 kb on - strand, within BWI76_RS10605 at 2240.338 kb on - strand, within BWI76_RS10605 at 2240.338 kb on - strand, within BWI76_RS10605 at 2240.382 kb on - strand, within BWI76_RS10605 at 2240.384 kb on + strand at 2240.389 kb on - strand, within BWI76_RS10605 at 2240.392 kb on - strand, within BWI76_RS10605 at 2240.392 kb on - strand at 2240.392 kb on - strand, within BWI76_RS10605 at 2240.397 kb on - strand, within BWI76_RS10605 at 2240.397 kb on - strand, within BWI76_RS10605 at 2240.404 kb on - strand, within BWI76_RS10605 at 2240.413 kb on + strand, within BWI76_RS10605 at 2240.426 kb on + strand, within BWI76_RS10605 at 2240.426 kb on + strand at 2240.426 kb on + strand, within BWI76_RS10605 at 2240.426 kb on + strand, within BWI76_RS10605 at 2240.426 kb on + strand, within BWI76_RS10605 at 2240.434 kb on - strand, within BWI76_RS10605 at 2240.434 kb on - strand, within BWI76_RS10605 at 2240.434 kb on - strand, within BWI76_RS10605 at 2240.442 kb on + strand, within BWI76_RS10605 at 2240.462 kb on + strand, within BWI76_RS10605 at 2240.462 kb on + strand, within BWI76_RS10605 at 2240.467 kb on - strand, within BWI76_RS10605 at 2240.470 kb on - strand, within BWI76_RS10605 at 2240.471 kb on + strand, within BWI76_RS10605 at 2240.471 kb on + strand, within BWI76_RS10605 at 2240.471 kb on + strand, within BWI76_RS10605 at 2240.479 kb on - strand, within BWI76_RS10605 at 2240.525 kb on + strand, within BWI76_RS10605 at 2240.525 kb on + strand, within BWI76_RS10605 at 2240.525 kb on + strand, within BWI76_RS10605 at 2240.525 kb on + strand, within BWI76_RS10605 at 2240.525 kb on + strand, within BWI76_RS10605 at 2240.525 kb on + strand, within BWI76_RS10605 at 2240.533 kb on - strand, within BWI76_RS10605 at 2240.578 kb on + strand, within BWI76_RS10605 at 2240.603 kb on + strand at 2240.603 kb on + strand, within BWI76_RS10605 at 2240.611 kb on + strand, within BWI76_RS10605 at 2240.637 kb on - strand, within BWI76_RS10605 at 2240.639 kb on - strand, within BWI76_RS10605 at 2240.643 kb on + strand, within BWI76_RS10605 at 2240.687 kb on + strand at 2240.687 kb on + strand at 2240.687 kb on + strand at 2240.687 kb on + strand at 2240.687 kb on + strand at 2240.687 kb on + strand at 2240.687 kb on + strand at 2240.695 kb on + strand at 2240.716 kb on - strand at 2240.813 kb on - strand at 2240.813 kb on - strand at 2240.817 kb on - strand at 2240.846 kb on + strand at 2240.846 kb on + strand at 2240.856 kb on + strand at 2240.856 kb on + strand at 2240.857 kb on - strand at 2240.872 kb on - strand at 2240.895 kb on - strand at 2240.918 kb on + strand at 2240.924 kb on + strand at 2240.938 kb on - strand at 2240.938 kb on - strand at 2240.951 kb on + strand at 2240.953 kb on + strand at 2240.953 kb on + strand at 2240.953 kb on + strand at 2240.953 kb on + strand at 2240.953 kb on + strand at 2240.953 kb on + strand at 2240.953 kb on + strand at 2240.961 kb on - strand at 2240.961 kb on - strand at 2240.961 kb on - strand at 2240.981 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 2,237,402 + BWI76_RS10595 0.35 -0.5 2,237,518 + BWI76_RS10595 0.44 -1.5 2,237,603 - BWI76_RS10595 0.51 +0.2 2,237,660 + BWI76_RS10595 0.56 -0.6 2,237,826 + BWI76_RS10595 0.69 -1.3 2,238,015 + BWI76_RS10595 0.84 +0.6 2,238,165 - -0.8 2,238,252 - +0.1 2,238,290 + -0.5 2,238,298 - -0.2 2,238,577 + BWI76_RS10600 0.18 +0.8 2,238,697 + BWI76_RS10600 0.25 +0.2 2,238,896 + BWI76_RS10600 0.36 -2.7 2,238,901 + BWI76_RS10600 0.37 -1.1 2,238,904 - BWI76_RS10600 0.37 +0.5 2,238,933 + BWI76_RS10600 0.39 +0.7 2,239,140 + BWI76_RS10600 0.51 +0.0 2,239,218 - BWI76_RS10600 0.55 +0.7 2,239,396 + BWI76_RS10600 0.66 +1.1 2,239,495 + BWI76_RS10600 0.71 +0.8 2,239,604 + BWI76_RS10600 0.78 +0.1 2,239,611 + BWI76_RS10600 0.78 -0.8 2,239,612 - BWI76_RS10600 0.78 +0.5 2,239,710 - BWI76_RS10600 0.84 +1.0 2,239,710 - BWI76_RS10600 0.84 -0.5 2,239,747 + BWI76_RS10600 0.86 -1.8 2,239,747 - BWI76_RS10600 0.86 +0.3 2,239,763 + BWI76_RS10600 0.87 -1.7 2,239,809 + BWI76_RS10600 0.90 -0.2 2,239,817 - +0.5 2,239,827 + -0.5 2,239,830 + +0.6 2,239,830 + +0.2 2,239,837 + -1.9 2,239,872 - +0.1 2,239,887 + +0.6 2,239,887 + -0.4 2,239,887 + -0.1 2,239,887 + -1.9 2,239,887 + +0.2 2,239,887 + +0.4 2,239,887 + -0.1 2,239,887 + +0.2 2,239,887 + +0.1 2,239,887 + -0.3 2,239,887 + +0.1 2,239,887 + -0.6 2,239,887 + -0.1 2,239,887 + -2.0 2,239,887 + -0.6 2,239,887 + +0.1 2,239,887 + +1.1 2,239,887 + +0.4 2,239,895 - -0.3 2,239,895 - -0.3 2,239,895 - -0.2 2,239,895 - +0.4 2,239,895 - +0.6 2,239,895 - -0.1 2,239,895 - -0.6 2,239,895 - -0.3 2,239,895 - +0.3 2,239,895 - -0.4 2,239,895 - +0.2 2,239,895 - +0.5 2,239,895 - -1.5 2,239,895 - +1.9 2,239,897 + +0.3 2,239,897 + -0.5 2,239,897 + +0.1 2,239,897 + -1.0 2,239,897 + +0.7 2,239,897 + -1.4 2,239,897 + +0.8 2,239,897 + +0.4 2,239,897 + +0.3 2,239,897 + -1.1 2,239,897 + -0.3 2,239,897 + -0.2 2,239,897 + -0.6 2,239,897 + +0.8 2,239,897 + -0.8 2,239,897 + +1.7 2,239,897 + -0.9 2,239,897 + +0.5 2,239,905 - -0.1 2,239,905 - -0.5 2,239,905 - +0.4 2,239,905 - -2.1 2,239,905 - -1.5 2,239,905 - +0.1 2,239,905 - -1.5 2,239,905 - -0.4 2,239,905 - +0.3 2,239,905 - -1.5 2,239,905 - +2.7 2,239,905 - +1.1 2,239,907 + +0.2 2,239,912 + -0.8 2,239,915 - -0.2 2,239,920 + -0.1 2,239,922 - +0.0 2,239,937 + -0.3 2,239,940 - -2.7 2,239,952 + -0.2 2,239,952 + -0.7 2,239,952 + -0.5 2,239,952 + +0.2 2,239,952 + -1.1 2,239,952 + -1.8 2,239,960 - -0.9 2,239,960 - +0.5 2,239,960 - +0.3 2,240,001 + -0.6 2,240,016 - +0.9 2,240,029 + -0.5 2,240,051 - -1.5 2,240,067 + -3.5 2,240,086 - +0.2 2,240,086 - -0.6 2,240,088 + +0.1 2,240,096 - -2.0 2,240,096 - -0.9 2,240,096 - -0.5 2,240,096 - -0.0 2,240,096 - -0.4 2,240,104 - +0.2 2,240,131 - BWI76_RS10605 0.11 -1.5 2,240,182 - BWI76_RS10605 0.18 +0.9 2,240,200 - BWI76_RS10605 0.21 -0.1 2,240,291 + BWI76_RS10605 0.34 +1.5 2,240,291 + BWI76_RS10605 0.34 +0.7 2,240,299 - BWI76_RS10605 0.35 -0.5 2,240,299 - BWI76_RS10605 0.35 +0.6 2,240,301 + BWI76_RS10605 0.35 +0.5 2,240,306 + BWI76_RS10605 0.36 +0.8 2,240,306 + BWI76_RS10605 0.36 -0.8 2,240,306 + BWI76_RS10605 0.36 +0.1 2,240,306 + BWI76_RS10605 0.36 +0.1 2,240,306 + BWI76_RS10605 0.36 -0.5 2,240,306 + BWI76_RS10605 0.36 -1.7 2,240,314 - BWI76_RS10605 0.37 -0.5 2,240,314 - BWI76_RS10605 0.37 -3.3 2,240,314 - BWI76_RS10605 0.37 +0.5 2,240,314 - BWI76_RS10605 0.37 +0.1 2,240,314 - BWI76_RS10605 0.37 +1.1 2,240,314 - BWI76_RS10605 0.37 -0.2 2,240,314 - BWI76_RS10605 0.37 +0.1 2,240,314 - BWI76_RS10605 0.37 -0.5 2,240,314 - BWI76_RS10605 0.37 -1.3 2,240,314 - BWI76_RS10605 0.37 +0.8 2,240,314 - BWI76_RS10605 0.37 -1.2 2,240,314 - BWI76_RS10605 0.37 +1.1 2,240,314 - BWI76_RS10605 0.37 -1.1 2,240,314 - BWI76_RS10605 0.37 -0.8 2,240,314 - BWI76_RS10605 0.37 -0.6 2,240,314 - BWI76_RS10605 0.37 -0.4 2,240,314 - BWI76_RS10605 0.37 -2.9 2,240,314 - BWI76_RS10605 0.37 +1.3 2,240,314 - BWI76_RS10605 0.37 +1.1 2,240,314 - BWI76_RS10605 0.37 -0.3 2,240,314 - BWI76_RS10605 0.37 -2.1 2,240,328 + BWI76_RS10605 0.39 -0.7 2,240,330 + BWI76_RS10605 0.39 -1.5 2,240,330 + BWI76_RS10605 0.39 +0.1 2,240,336 - BWI76_RS10605 0.40 -0.5 2,240,336 - BWI76_RS10605 0.40 -1.3 2,240,336 - BWI76_RS10605 0.40 -0.3 2,240,336 - BWI76_RS10605 0.40 -0.5 2,240,338 - BWI76_RS10605 0.40 -2.6 2,240,338 - BWI76_RS10605 0.40 -1.5 2,240,338 - BWI76_RS10605 0.40 +0.1 2,240,338 - BWI76_RS10605 0.40 +1.4 2,240,338 - BWI76_RS10605 0.40 +0.7 2,240,382 - BWI76_RS10605 0.47 -0.5 2,240,384 + +1.1 2,240,389 - BWI76_RS10605 0.48 -0.4 2,240,392 - BWI76_RS10605 0.48 -1.9 2,240,392 - +3.7 2,240,392 - BWI76_RS10605 0.48 +2.8 2,240,397 - BWI76_RS10605 0.49 -2.5 2,240,397 - BWI76_RS10605 0.49 -2.1 2,240,404 - BWI76_RS10605 0.50 -1.7 2,240,413 + BWI76_RS10605 0.51 +0.1 2,240,426 + BWI76_RS10605 0.53 -1.5 2,240,426 + +2.4 2,240,426 + BWI76_RS10605 0.53 +0.4 2,240,426 + BWI76_RS10605 0.53 -0.3 2,240,426 + BWI76_RS10605 0.53 -2.3 2,240,434 - BWI76_RS10605 0.54 -1.4 2,240,434 - BWI76_RS10605 0.54 +1.1 2,240,434 - BWI76_RS10605 0.54 -2.5 2,240,442 + BWI76_RS10605 0.55 -0.5 2,240,462 + BWI76_RS10605 0.58 -1.3 2,240,462 + BWI76_RS10605 0.58 -2.5 2,240,467 - BWI76_RS10605 0.59 -0.9 2,240,470 - BWI76_RS10605 0.59 -1.6 2,240,471 + BWI76_RS10605 0.59 -1.5 2,240,471 + BWI76_RS10605 0.59 +1.1 2,240,471 + BWI76_RS10605 0.59 +0.2 2,240,479 - BWI76_RS10605 0.60 +0.4 2,240,525 + BWI76_RS10605 0.67 -3.2 2,240,525 + BWI76_RS10605 0.67 +0.1 2,240,525 + BWI76_RS10605 0.67 +1.1 2,240,525 + BWI76_RS10605 0.67 +0.1 2,240,525 + BWI76_RS10605 0.67 +0.1 2,240,525 + BWI76_RS10605 0.67 +1.2 2,240,533 - BWI76_RS10605 0.68 +1.7 2,240,578 + BWI76_RS10605 0.75 -0.5 2,240,603 + -0.5 2,240,603 + BWI76_RS10605 0.78 +1.1 2,240,611 + BWI76_RS10605 0.79 +1.1 2,240,637 - BWI76_RS10605 0.83 -1.6 2,240,639 - BWI76_RS10605 0.83 -0.4 2,240,643 + BWI76_RS10605 0.84 -1.3 2,240,687 + +2.1 2,240,687 + -0.9 2,240,687 + -0.3 2,240,687 + +0.8 2,240,687 + -0.9 2,240,687 + +1.1 2,240,687 + -2.7 2,240,695 + -0.3 2,240,716 - -0.1 2,240,813 - -3.8 2,240,813 - -1.0 2,240,817 - +1.7 2,240,846 + +0.1 2,240,846 + +0.1 2,240,856 + -0.9 2,240,856 + -1.5 2,240,857 - -2.5 2,240,872 - -1.4 2,240,895 - +3.1 2,240,918 + -1.5 2,240,924 + +2.4 2,240,938 - +1.1 2,240,938 - -0.5 2,240,951 + +0.1 2,240,953 + -1.9 2,240,953 + -1.3 2,240,953 + +1.1 2,240,953 + +0.1 2,240,953 + +0.7 2,240,953 + +2.7 2,240,953 + +1.6 2,240,961 - -1.5 2,240,961 - +0.6 2,240,961 - -0.1 2,240,981 - +0.8
Or see this region's nucleotide sequence