Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10025 and BWI76_RS10030 are separated by 235 nucleotides BWI76_RS10030 and BWI76_RS10035 are separated by 42 nucleotides
BWI76_RS10025: BWI76_RS10025 - secretion protein HlyD, at 2,122,349 to 2,123,554
_RS10025
BWI76_RS10030: BWI76_RS10030 - glycosyl transferase, at 2,123,790 to 2,126,933
_RS10030
BWI76_RS10035: BWI76_RS10035 - hypothetical protein, at 2,126,976 to 2,127,908
_RS10035
Position (kb)
2123
2124
2125
2126
2127 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2122.888 kb on - strand, within BWI76_RS10025 at 2122.926 kb on + strand, within BWI76_RS10025 at 2122.934 kb on - strand, within BWI76_RS10025 at 2122.941 kb on + strand, within BWI76_RS10025 at 2122.949 kb on - strand, within BWI76_RS10025 at 2123.018 kb on - strand, within BWI76_RS10025 at 2123.023 kb on + strand, within BWI76_RS10025 at 2123.046 kb on - strand, within BWI76_RS10025 at 2123.052 kb on - strand, within BWI76_RS10025 at 2123.057 kb on - strand, within BWI76_RS10025 at 2123.065 kb on - strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.097 kb on - strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.137 kb on + strand, within BWI76_RS10025 at 2123.154 kb on + strand, within BWI76_RS10025 at 2123.164 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.214 kb on - strand, within BWI76_RS10025 at 2123.256 kb on - strand, within BWI76_RS10025 at 2123.324 kb on + strand, within BWI76_RS10025 at 2123.339 kb on - strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.356 kb on - strand, within BWI76_RS10025 at 2123.356 kb on - strand, within BWI76_RS10025 at 2123.356 kb on - strand, within BWI76_RS10025 at 2123.392 kb on + strand, within BWI76_RS10025 at 2123.410 kb on - strand, within BWI76_RS10025 at 2123.456 kb on - strand at 2123.473 kb on + strand at 2123.527 kb on + strand at 2123.535 kb on - strand at 2123.565 kb on + strand at 2123.570 kb on + strand at 2123.570 kb on + strand at 2123.573 kb on - strand at 2123.578 kb on - strand at 2123.578 kb on - strand at 2123.610 kb on + strand at 2123.610 kb on + strand at 2123.610 kb on + strand at 2123.610 kb on + strand at 2123.613 kb on - strand at 2123.615 kb on + strand at 2123.615 kb on + strand at 2123.618 kb on - strand at 2123.623 kb on - strand at 2123.623 kb on - strand at 2123.665 kb on - strand at 2123.681 kb on - strand at 2123.684 kb on + strand at 2123.692 kb on - strand at 2123.694 kb on - strand at 2123.696 kb on + strand at 2123.704 kb on - strand at 2123.763 kb on - strand at 2123.789 kb on - strand at 2123.791 kb on - strand at 2123.803 kb on + strand at 2123.863 kb on - strand at 2123.864 kb on - strand at 2123.864 kb on - strand at 2123.880 kb on + strand at 2123.966 kb on + strand at 2124.176 kb on + strand, within BWI76_RS10030 at 2124.184 kb on - strand, within BWI76_RS10030 at 2124.367 kb on - strand, within BWI76_RS10030 at 2124.537 kb on - strand, within BWI76_RS10030 at 2124.562 kb on + strand, within BWI76_RS10030 at 2124.936 kb on - strand, within BWI76_RS10030 at 2124.955 kb on + strand, within BWI76_RS10030 at 2125.278 kb on + strand, within BWI76_RS10030 at 2125.534 kb on + strand, within BWI76_RS10030 at 2125.541 kb on + strand, within BWI76_RS10030 at 2125.714 kb on + strand, within BWI76_RS10030 at 2125.759 kb on + strand, within BWI76_RS10030 at 2125.824 kb on - strand, within BWI76_RS10030 at 2125.943 kb on + strand, within BWI76_RS10030 at 2126.146 kb on - strand, within BWI76_RS10030 at 2126.443 kb on + strand, within BWI76_RS10030 at 2126.451 kb on - strand, within BWI76_RS10030 at 2126.451 kb on - strand at 2126.503 kb on - strand, within BWI76_RS10030 at 2126.770 kb on + strand at 2126.859 kb on + strand at 2126.880 kb on + strand at 2126.888 kb on - strand at 2127.018 kb on - strand at 2127.267 kb on + strand, within BWI76_RS10035 at 2127.326 kb on + strand, within BWI76_RS10035 at 2127.835 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 2,122,888 - BWI76_RS10025 0.45 +0.5 2,122,926 + BWI76_RS10025 0.48 +0.6 2,122,934 - BWI76_RS10025 0.49 +0.5 2,122,941 + BWI76_RS10025 0.49 -0.1 2,122,949 - BWI76_RS10025 0.50 -1.2 2,123,018 - BWI76_RS10025 0.55 -0.4 2,123,023 + BWI76_RS10025 0.56 -1.4 2,123,046 - BWI76_RS10025 0.58 +1.7 2,123,052 - BWI76_RS10025 0.58 -0.1 2,123,057 - BWI76_RS10025 0.59 +1.1 2,123,065 - BWI76_RS10025 0.59 -0.5 2,123,090 + BWI76_RS10025 0.61 -2.1 2,123,090 + BWI76_RS10025 0.61 -0.0 2,123,090 + BWI76_RS10025 0.61 -0.6 2,123,097 - BWI76_RS10025 0.62 +0.1 2,123,111 + BWI76_RS10025 0.63 -0.4 2,123,111 + BWI76_RS10025 0.63 -1.7 2,123,111 + BWI76_RS10025 0.63 -1.2 2,123,119 - BWI76_RS10025 0.64 -0.7 2,123,119 - BWI76_RS10025 0.64 -1.9 2,123,119 - BWI76_RS10025 0.64 -1.2 2,123,137 + BWI76_RS10025 0.65 -0.9 2,123,154 + BWI76_RS10025 0.67 -1.0 2,123,164 + BWI76_RS10025 0.68 -0.7 2,123,169 + BWI76_RS10025 0.68 -0.5 2,123,169 + BWI76_RS10025 0.68 +0.1 2,123,169 + BWI76_RS10025 0.68 -0.0 2,123,169 + BWI76_RS10025 0.68 +0.6 2,123,169 + BWI76_RS10025 0.68 +0.3 2,123,177 - BWI76_RS10025 0.69 -1.1 2,123,177 - BWI76_RS10025 0.69 -2.5 2,123,177 - BWI76_RS10025 0.69 -0.9 2,123,214 - BWI76_RS10025 0.72 -0.4 2,123,256 - BWI76_RS10025 0.75 +0.7 2,123,324 + BWI76_RS10025 0.81 -0.3 2,123,339 - BWI76_RS10025 0.82 -0.9 2,123,348 + BWI76_RS10025 0.83 -0.7 2,123,348 + BWI76_RS10025 0.83 -0.1 2,123,348 + BWI76_RS10025 0.83 -1.5 2,123,356 - BWI76_RS10025 0.83 +0.2 2,123,356 - BWI76_RS10025 0.83 -3.4 2,123,356 - BWI76_RS10025 0.83 +1.1 2,123,392 + BWI76_RS10025 0.86 -0.3 2,123,410 - BWI76_RS10025 0.88 +0.8 2,123,456 - -0.3 2,123,473 + -0.4 2,123,527 + +0.6 2,123,535 - -0.9 2,123,565 + +0.8 2,123,570 + -0.2 2,123,570 + -0.5 2,123,573 - -0.2 2,123,578 - +0.8 2,123,578 - +0.6 2,123,610 + +0.1 2,123,610 + +0.3 2,123,610 + -0.5 2,123,610 + -1.2 2,123,613 - -1.2 2,123,615 + -0.7 2,123,615 + +0.1 2,123,618 - +0.2 2,123,623 - -0.9 2,123,623 - -0.1 2,123,665 - +1.1 2,123,681 - +0.2 2,123,684 + -1.0 2,123,692 - -1.2 2,123,694 - +0.6 2,123,696 + +0.1 2,123,704 - +0.2 2,123,763 - -0.4 2,123,789 - -1.2 2,123,791 - -0.0 2,123,803 + -0.8 2,123,863 - -0.3 2,123,864 - +2.1 2,123,864 - -1.4 2,123,880 + -0.5 2,123,966 + -0.3 2,124,176 + BWI76_RS10030 0.12 -0.1 2,124,184 - BWI76_RS10030 0.13 -0.1 2,124,367 - BWI76_RS10030 0.18 +0.7 2,124,537 - BWI76_RS10030 0.24 +1.5 2,124,562 + BWI76_RS10030 0.25 +1.1 2,124,936 - BWI76_RS10030 0.36 -0.3 2,124,955 + BWI76_RS10030 0.37 +0.5 2,125,278 + BWI76_RS10030 0.47 +0.1 2,125,534 + BWI76_RS10030 0.55 -0.2 2,125,541 + BWI76_RS10030 0.56 -0.0 2,125,714 + BWI76_RS10030 0.61 -0.2 2,125,759 + BWI76_RS10030 0.63 -0.4 2,125,824 - BWI76_RS10030 0.65 -0.3 2,125,943 + BWI76_RS10030 0.68 -1.8 2,126,146 - BWI76_RS10030 0.75 -0.8 2,126,443 + BWI76_RS10030 0.84 +0.1 2,126,451 - BWI76_RS10030 0.85 -0.4 2,126,451 - +2.1 2,126,503 - BWI76_RS10030 0.86 -0.6 2,126,770 + -0.1 2,126,859 + +1.1 2,126,880 + -0.1 2,126,888 - +1.1 2,127,018 - -0.2 2,127,267 + BWI76_RS10035 0.31 -1.7 2,127,326 + BWI76_RS10035 0.38 -1.1 2,127,835 + +0.1
Or see this region's nucleotide sequence