Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09550 and BWI76_RS09555 are separated by 154 nucleotides BWI76_RS09555 and BWI76_RS09560 are separated by 151 nucleotides BWI76_RS09560 and BWI76_RS09565 are separated by 39 nucleotides
BWI76_RS09550: BWI76_RS09550 - low-specificity L-threonine aldolase, at 1,997,268 to 1,998,269
_RS09550
BWI76_RS09555: BWI76_RS09555 - pyruvate oxidase, at 1,998,424 to 2,000,142
_RS09555
BWI76_RS09560: BWI76_RS09560 - DoxX family membrane protein, at 2,000,294 to 2,000,728
_RS09560
BWI76_RS09565: BWI76_RS09565 - hybrid-cluster NAD(P)-dependent oxidoreductase, at 2,000,768 to 2,001,736
_RS09565
Position (kb) 1998
1999
2000
2001 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1997.556 kb on - strand, within BWI76_RS09550 at 1997.744 kb on - strand, within BWI76_RS09550 at 1997.750 kb on - strand, within BWI76_RS09550 at 1998.198 kb on - strand at 1998.235 kb on + strand at 1998.310 kb on - strand at 1998.320 kb on + strand at 1998.492 kb on + strand at 1998.919 kb on - strand, within BWI76_RS09555 at 1999.118 kb on - strand, within BWI76_RS09555 at 1999.411 kb on - strand, within BWI76_RS09555 at 1999.717 kb on + strand, within BWI76_RS09555 at 2000.108 kb on - strand at 2000.178 kb on + strand at 2000.441 kb on - strand, within BWI76_RS09560 at 2000.602 kb on - strand, within BWI76_RS09560 at 2000.916 kb on - strand, within BWI76_RS09565 at 2000.965 kb on + strand, within BWI76_RS09565
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 1,997,556 - BWI76_RS09550 0.29 +0.1 1,997,744 - BWI76_RS09550 0.48 +0.4 1,997,750 - BWI76_RS09550 0.48 +0.6 1,998,198 - +0.2 1,998,235 + +0.5 1,998,310 - +0.1 1,998,320 + -0.5 1,998,492 + +0.4 1,998,919 - BWI76_RS09555 0.29 -1.2 1,999,118 - BWI76_RS09555 0.40 -0.9 1,999,411 - BWI76_RS09555 0.57 -0.4 1,999,717 + BWI76_RS09555 0.75 +0.8 2,000,108 - -0.9 2,000,178 + +1.8 2,000,441 - BWI76_RS09560 0.34 +0.7 2,000,602 - BWI76_RS09560 0.71 -2.5 2,000,916 - BWI76_RS09565 0.15 +0.6 2,000,965 + BWI76_RS09565 0.20 +0.3
Or see this region's nucleotide sequence