Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09075 and BWI76_RS09080 are separated by 152 nucleotides BWI76_RS09080 and BWI76_RS09085 overlap by 1 nucleotides BWI76_RS09085 and BWI76_RS09090 are separated by 177 nucleotides
BWI76_RS09075: BWI76_RS09075 - pyruvate formate lyase-activating protein, at 1,916,043 to 1,916,942
_RS09075
BWI76_RS09080: BWI76_RS09080 - molybdopterin-synthase adenylyltransferase MoeB, at 1,917,095 to 1,917,850
_RS09080
BWI76_RS09085: BWI76_RS09085 - molybdopterin molybdenumtransferase MoeA, at 1,917,850 to 1,919,085
_RS09085
BWI76_RS09090: BWI76_RS09090 - beta-aspartyl-peptidase, at 1,919,263 to 1,920,204
_RS09090
Position (kb) 1917
1918
1919
1920 Strain fitness (log2 ratio) -2
-1
0
1 at 1917.117 kb on + strand at 1917.343 kb on - strand, within BWI76_RS09080 at 1917.670 kb on + strand, within BWI76_RS09080 at 1917.818 kb on - strand at 1917.838 kb on + strand at 1917.865 kb on - strand at 1918.082 kb on - strand, within BWI76_RS09085 at 1918.231 kb on + strand, within BWI76_RS09085 at 1918.347 kb on - strand, within BWI76_RS09085 at 1918.418 kb on + strand, within BWI76_RS09085 at 1918.435 kb on + strand, within BWI76_RS09085 at 1918.585 kb on - strand, within BWI76_RS09085 at 1918.846 kb on + strand, within BWI76_RS09085 at 1919.054 kb on - strand at 1919.139 kb on - strand at 1919.319 kb on + strand at 1919.343 kb on + strand at 1919.348 kb on - strand at 1919.353 kb on - strand at 1919.403 kb on - strand, within BWI76_RS09090 at 1919.416 kb on - strand, within BWI76_RS09090 at 1919.476 kb on - strand, within BWI76_RS09090 at 1919.975 kb on - strand, within BWI76_RS09090 at 1920.075 kb on + strand, within BWI76_RS09090
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 1,917,117 + +0.6 1,917,343 - BWI76_RS09080 0.33 +0.4 1,917,670 + BWI76_RS09080 0.76 -0.1 1,917,818 - -0.9 1,917,838 + +0.9 1,917,865 - +0.1 1,918,082 - BWI76_RS09085 0.19 +0.5 1,918,231 + BWI76_RS09085 0.31 +0.3 1,918,347 - BWI76_RS09085 0.40 +1.0 1,918,418 + BWI76_RS09085 0.46 +0.9 1,918,435 + BWI76_RS09085 0.47 +0.5 1,918,585 - BWI76_RS09085 0.59 +0.5 1,918,846 + BWI76_RS09085 0.81 +1.1 1,919,054 - +0.3 1,919,139 - +0.4 1,919,319 + -2.1 1,919,343 + -1.9 1,919,348 - +0.0 1,919,353 - -0.2 1,919,403 - BWI76_RS09090 0.15 -0.5 1,919,416 - BWI76_RS09090 0.16 -0.9 1,919,476 - BWI76_RS09090 0.23 +0.4 1,919,975 - BWI76_RS09090 0.76 -2.0 1,920,075 + BWI76_RS09090 0.86 +0.3
Or see this region's nucleotide sequence