Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08075

Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08065 and BWI76_RS08070 overlap by 1 nucleotidesBWI76_RS08070 and BWI76_RS08075 are separated by 14 nucleotidesBWI76_RS08075 and BWI76_RS08080 are separated by 202 nucleotidesBWI76_RS08080 and BWI76_RS08085 are separated by 137 nucleotides BWI76_RS08065: BWI76_RS08065 - DNA polymerase III subunit delta, at 1,707,395 to 1,708,426 _RS08065 BWI76_RS08070: BWI76_RS08070 - LPS assembly lipoprotein LptE, at 1,708,426 to 1,709,016 _RS08070 BWI76_RS08075: BWI76_RS08075 - leucine--tRNA ligase, at 1,709,031 to 1,711,637 _RS08075 BWI76_RS08080: BWI76_RS08080 - hypothetical protein, at 1,711,840 to 1,712,322 _RS08080 BWI76_RS08085: BWI76_RS08085 - putative Filamentous hemagglutinin, at 1,712,460 to 1,714,253 _RS08085 Position (kb) 1709 1710 1711 1712Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1711.714 kb on + strandat 1711.862 kb on + strandat 1711.908 kb on + strand, within BWI76_RS08080at 1711.948 kb on - strand, within BWI76_RS08080at 1711.949 kb on - strand, within BWI76_RS08080at 1711.954 kb on - strand, within BWI76_RS08080at 1712.033 kb on + strand, within BWI76_RS08080at 1712.033 kb on + strand, within BWI76_RS08080at 1712.222 kb on - strand, within BWI76_RS08080at 1712.247 kb on - strand, within BWI76_RS08080at 1712.285 kb on + strandat 1712.338 kb on - strandat 1712.339 kb on - strandat 1712.344 kb on - strandat 1712.364 kb on - strandat 1712.366 kb on - strandat 1712.510 kb on + strandat 1712.510 kb on + strandat 1712.510 kb on + strandat 1712.510 kb on + strandat 1712.510 kb on + strandat 1712.510 kb on + strandat 1712.518 kb on - strandat 1712.518 kb on - strandat 1712.518 kb on - strandat 1712.518 kb on - strandat 1712.518 kb on - strandat 1712.530 kb on + strandat 1712.546 kb on + strandat 1712.546 kb on + strandat 1712.546 kb on + strandat 1712.554 kb on - strandat 1712.554 kb on - strandat 1712.554 kb on - strandat 1712.562 kb on - strandat 1712.565 kb on + strandat 1712.577 kb on + strandat 1712.583 kb on - strandat 1712.585 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
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1,711,714 + +0.1
1,711,862 + +2.5
1,711,908 + BWI76_RS08080 0.14 +0.7
1,711,948 - BWI76_RS08080 0.22 +0.5
1,711,949 - BWI76_RS08080 0.23 +0.5
1,711,954 - BWI76_RS08080 0.24 -0.1
1,712,033 + BWI76_RS08080 0.40 +0.6
1,712,033 + BWI76_RS08080 0.40 -0.6
1,712,222 - BWI76_RS08080 0.79 +0.6
1,712,247 - BWI76_RS08080 0.84 +0.1
1,712,285 + +0.1
1,712,338 - +1.4
1,712,339 - +0.2
1,712,344 - -2.1
1,712,364 - +0.1
1,712,366 - +0.8
1,712,510 + -1.7
1,712,510 + -0.4
1,712,510 + +3.1
1,712,510 + -0.3
1,712,510 + -0.8
1,712,510 + -1.2
1,712,518 - -0.2
1,712,518 - -0.2
1,712,518 - +0.4
1,712,518 - +0.2
1,712,518 - -0.1
1,712,530 + +0.0
1,712,546 + +0.1
1,712,546 + -1.1
1,712,546 + +0.8
1,712,554 - -1.0
1,712,554 - -0.4
1,712,554 - -1.3
1,712,562 - -0.9
1,712,565 + +0.2
1,712,577 + -0.5
1,712,583 - +0.0
1,712,585 - +1.0

Or see this region's nucleotide sequence