Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08065 and BWI76_RS08070 overlap by 1 nucleotides BWI76_RS08070 and BWI76_RS08075 are separated by 14 nucleotides BWI76_RS08075 and BWI76_RS08080 are separated by 202 nucleotides BWI76_RS08080 and BWI76_RS08085 are separated by 137 nucleotides
BWI76_RS08065: BWI76_RS08065 - DNA polymerase III subunit delta, at 1,707,395 to 1,708,426
_RS08065
BWI76_RS08070: BWI76_RS08070 - LPS assembly lipoprotein LptE, at 1,708,426 to 1,709,016
_RS08070
BWI76_RS08075: BWI76_RS08075 - leucine--tRNA ligase, at 1,709,031 to 1,711,637
_RS08075
BWI76_RS08080: BWI76_RS08080 - hypothetical protein, at 1,711,840 to 1,712,322
_RS08080
BWI76_RS08085: BWI76_RS08085 - putative Filamentous hemagglutinin, at 1,712,460 to 1,714,253
_RS08085
Position (kb)
1709
1710
1711
1712 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1711.714 kb on + strand at 1711.862 kb on + strand at 1711.908 kb on + strand, within BWI76_RS08080 at 1711.948 kb on - strand, within BWI76_RS08080 at 1711.949 kb on - strand, within BWI76_RS08080 at 1711.954 kb on - strand, within BWI76_RS08080 at 1712.033 kb on + strand, within BWI76_RS08080 at 1712.033 kb on + strand, within BWI76_RS08080 at 1712.222 kb on - strand, within BWI76_RS08080 at 1712.247 kb on - strand, within BWI76_RS08080 at 1712.285 kb on + strand at 1712.338 kb on - strand at 1712.339 kb on - strand at 1712.344 kb on - strand at 1712.364 kb on - strand at 1712.366 kb on - strand at 1712.510 kb on + strand at 1712.510 kb on + strand at 1712.510 kb on + strand at 1712.510 kb on + strand at 1712.510 kb on + strand at 1712.510 kb on + strand at 1712.518 kb on - strand at 1712.518 kb on - strand at 1712.518 kb on - strand at 1712.518 kb on - strand at 1712.518 kb on - strand at 1712.530 kb on + strand at 1712.546 kb on + strand at 1712.546 kb on + strand at 1712.546 kb on + strand at 1712.554 kb on - strand at 1712.554 kb on - strand at 1712.554 kb on - strand at 1712.562 kb on - strand at 1712.565 kb on + strand at 1712.577 kb on + strand at 1712.583 kb on - strand at 1712.585 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 1,711,714 + +0.1 1,711,862 + +2.5 1,711,908 + BWI76_RS08080 0.14 +0.7 1,711,948 - BWI76_RS08080 0.22 +0.5 1,711,949 - BWI76_RS08080 0.23 +0.5 1,711,954 - BWI76_RS08080 0.24 -0.1 1,712,033 + BWI76_RS08080 0.40 +0.6 1,712,033 + BWI76_RS08080 0.40 -0.6 1,712,222 - BWI76_RS08080 0.79 +0.6 1,712,247 - BWI76_RS08080 0.84 +0.1 1,712,285 + +0.1 1,712,338 - +1.4 1,712,339 - +0.2 1,712,344 - -2.1 1,712,364 - +0.1 1,712,366 - +0.8 1,712,510 + -1.7 1,712,510 + -0.4 1,712,510 + +3.1 1,712,510 + -0.3 1,712,510 + -0.8 1,712,510 + -1.2 1,712,518 - -0.2 1,712,518 - -0.2 1,712,518 - +0.4 1,712,518 - +0.2 1,712,518 - -0.1 1,712,530 + +0.0 1,712,546 + +0.1 1,712,546 + -1.1 1,712,546 + +0.8 1,712,554 - -1.0 1,712,554 - -0.4 1,712,554 - -1.3 1,712,562 - -0.9 1,712,565 + +0.2 1,712,577 + -0.5 1,712,583 - +0.0 1,712,585 - +1.0
Or see this region's nucleotide sequence