Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06865 and BWI76_RS06870 are separated by 115 nucleotides BWI76_RS06870 and BWI76_RS06875 are separated by 201 nucleotides BWI76_RS06875 and BWI76_RS06880 are separated by 322 nucleotides BWI76_RS06880 and BWI76_RS06885 are separated by 1 nucleotides
BWI76_RS06865: BWI76_RS06865 - bifunctional UDP-sugar hydrolase/5'-nucleotidase, at 1,441,588 to 1,443,240
_RS06865
BWI76_RS06870: BWI76_RS06870 - aminoacyl-tRNA deacylase, at 1,443,356 to 1,443,835
_RS06870
BWI76_RS06875: BWI76_RS06875 - conjugal transfer protein TraB, at 1,444,037 to 1,444,831
_RS06875
BWI76_RS06880: BWI76_RS06880 - biopolymer transport protein ExbD/TolR, at 1,445,154 to 1,445,561
_RS06880
BWI76_RS06885: BWI76_RS06885 - TonB family protein, at 1,445,563 to 1,446,306
_RS06885
Position (kb) 1444
1445 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1443.048 kb on + strand, within BWI76_RS06865 at 1443.169 kb on - strand at 1443.241 kb on + strand at 1443.249 kb on - strand at 1443.266 kb on + strand at 1443.478 kb on - strand, within BWI76_RS06870 at 1443.714 kb on + strand, within BWI76_RS06870 at 1443.721 kb on + strand, within BWI76_RS06870 at 1443.864 kb on - strand at 1443.881 kb on - strand at 1443.917 kb on + strand at 1444.180 kb on + strand, within BWI76_RS06875 at 1444.180 kb on + strand, within BWI76_RS06875 at 1444.188 kb on - strand, within BWI76_RS06875 at 1444.188 kb on - strand, within BWI76_RS06875 at 1444.188 kb on - strand, within BWI76_RS06875 at 1444.188 kb on - strand, within BWI76_RS06875 at 1444.188 kb on - strand, within BWI76_RS06875 at 1444.279 kb on + strand, within BWI76_RS06875 at 1444.373 kb on + strand, within BWI76_RS06875 at 1444.381 kb on - strand, within BWI76_RS06875 at 1444.392 kb on + strand, within BWI76_RS06875 at 1444.567 kb on - strand, within BWI76_RS06875 at 1444.716 kb on - strand, within BWI76_RS06875 at 1444.726 kb on + strand, within BWI76_RS06875 at 1444.726 kb on + strand, within BWI76_RS06875 at 1444.734 kb on - strand, within BWI76_RS06875 at 1444.740 kb on + strand, within BWI76_RS06875 at 1444.758 kb on - strand at 1444.836 kb on + strand at 1445.279 kb on - strand, within BWI76_RS06880 at 1445.413 kb on + strand, within BWI76_RS06880 at 1445.454 kb on + strand, within BWI76_RS06880
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 1,443,048 + BWI76_RS06865 0.88 +0.7 1,443,169 - -0.5 1,443,241 + -0.3 1,443,249 - +2.6 1,443,266 + -1.3 1,443,478 - BWI76_RS06870 0.25 -0.9 1,443,714 + BWI76_RS06870 0.75 -0.8 1,443,721 + BWI76_RS06870 0.76 -0.2 1,443,864 - +1.1 1,443,881 - +0.5 1,443,917 + -0.1 1,444,180 + BWI76_RS06875 0.18 -1.0 1,444,180 + BWI76_RS06875 0.18 -1.9 1,444,188 - BWI76_RS06875 0.19 +0.2 1,444,188 - BWI76_RS06875 0.19 -1.7 1,444,188 - BWI76_RS06875 0.19 +0.4 1,444,188 - BWI76_RS06875 0.19 +0.5 1,444,188 - BWI76_RS06875 0.19 -0.6 1,444,279 + BWI76_RS06875 0.30 -2.0 1,444,373 + BWI76_RS06875 0.42 -1.1 1,444,381 - BWI76_RS06875 0.43 -1.5 1,444,392 + BWI76_RS06875 0.45 +1.4 1,444,567 - BWI76_RS06875 0.67 -0.6 1,444,716 - BWI76_RS06875 0.85 -0.7 1,444,726 + BWI76_RS06875 0.87 -0.6 1,444,726 + BWI76_RS06875 0.87 -1.9 1,444,734 - BWI76_RS06875 0.88 -1.9 1,444,740 + BWI76_RS06875 0.88 +0.2 1,444,758 - +0.0 1,444,836 + -3.2 1,445,279 - BWI76_RS06880 0.31 +0.1 1,445,413 + BWI76_RS06880 0.63 +0.2 1,445,454 + BWI76_RS06880 0.74 -2.6
Or see this region's nucleotide sequence