Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06065 and BWI76_RS06070 are separated by 11 nucleotides BWI76_RS06070 and BWI76_RS06075 overlap by 4 nucleotides BWI76_RS06075 and BWI76_RS06080 overlap by 4 nucleotides
BWI76_RS06065: BWI76_RS06065 - taurine ABC transporter substrate-binding protein, at 1,274,898 to 1,275,860
_RS06065
BWI76_RS06070: BWI76_RS06070 - taurine transporter ATP-binding subunit, at 1,275,872 to 1,276,639
_RS06070
BWI76_RS06075: BWI76_RS06075 - taurine transporter subunit, at 1,276,636 to 1,277,463
_RS06075
BWI76_RS06080: BWI76_RS06080 - taurine dioxygenase, at 1,277,460 to 1,278,311
_RS06080
Position (kb) 1275
1276
1277 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1274.990 kb on - strand at 1275.225 kb on + strand, within BWI76_RS06065 at 1275.266 kb on + strand, within BWI76_RS06065 at 1275.302 kb on - strand, within BWI76_RS06065 at 1275.326 kb on - strand, within BWI76_RS06065 at 1275.355 kb on + strand, within BWI76_RS06065 at 1275.462 kb on + strand, within BWI76_RS06065 at 1275.507 kb on - strand, within BWI76_RS06065 at 1275.512 kb on + strand, within BWI76_RS06065 at 1275.767 kb on + strand at 1276.057 kb on - strand, within BWI76_RS06070 at 1276.071 kb on - strand, within BWI76_RS06070 at 1276.071 kb on - strand, within BWI76_RS06070 at 1276.143 kb on - strand, within BWI76_RS06070 at 1276.221 kb on + strand, within BWI76_RS06070 at 1276.606 kb on - strand at 1276.708 kb on + strand at 1276.735 kb on + strand, within BWI76_RS06075 at 1277.315 kb on + strand, within BWI76_RS06075
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 1,274,990 - +0.7 1,275,225 + BWI76_RS06065 0.34 -2.6 1,275,266 + BWI76_RS06065 0.38 -0.8 1,275,302 - BWI76_RS06065 0.42 -1.5 1,275,326 - BWI76_RS06065 0.44 -0.6 1,275,355 + BWI76_RS06065 0.47 +0.6 1,275,462 + BWI76_RS06065 0.59 +1.0 1,275,507 - BWI76_RS06065 0.63 +0.9 1,275,512 + BWI76_RS06065 0.64 +1.8 1,275,767 + +0.4 1,276,057 - BWI76_RS06070 0.24 -0.4 1,276,071 - BWI76_RS06070 0.26 +2.2 1,276,071 - BWI76_RS06070 0.26 +0.4 1,276,143 - BWI76_RS06070 0.35 +0.5 1,276,221 + BWI76_RS06070 0.45 +0.6 1,276,606 - +0.3 1,276,708 + +0.2 1,276,735 + BWI76_RS06075 0.12 +0.4 1,277,315 + BWI76_RS06075 0.82 -1.1
Or see this region's nucleotide sequence