Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05465 and BWI76_RS05470 are separated by 182 nucleotides BWI76_RS05470 and BWI76_RS05475 are separated by 238 nucleotides BWI76_RS05475 and BWI76_RS05480 are separated by 43 nucleotides
BWI76_RS05465: BWI76_RS05465 - amidohydrolase, at 1,145,681 to 1,146,451
_RS05465
BWI76_RS05470: BWI76_RS05470 - acyl-CoA dehydrogenase, at 1,146,634 to 1,149,078
_RS05470
BWI76_RS05475: BWI76_RS05475 - phosphoheptose isomerase, at 1,149,317 to 1,149,898
_RS05475
BWI76_RS05480: BWI76_RS05480 - class II glutamine amidotransferase, at 1,149,942 to 1,150,709
_RS05480
Position (kb) 1146
1147
1148
1149
1150 Strain fitness (log2 ratio) -2
-1
0
1 at 1145.997 kb on - strand, within BWI76_RS05465 at 1146.396 kb on - strand at 1146.490 kb on + strand at 1146.982 kb on - strand, within BWI76_RS05470 at 1147.030 kb on + strand, within BWI76_RS05470 at 1147.132 kb on - strand, within BWI76_RS05470 at 1147.231 kb on + strand, within BWI76_RS05470 at 1147.317 kb on + strand, within BWI76_RS05470 at 1147.347 kb on - strand, within BWI76_RS05470 at 1147.395 kb on - strand, within BWI76_RS05470 at 1147.421 kb on - strand, within BWI76_RS05470 at 1147.428 kb on + strand, within BWI76_RS05470 at 1147.945 kb on - strand, within BWI76_RS05470 at 1148.018 kb on + strand, within BWI76_RS05470 at 1148.123 kb on + strand, within BWI76_RS05470 at 1148.147 kb on - strand, within BWI76_RS05470 at 1148.429 kb on + strand, within BWI76_RS05470 at 1148.462 kb on - strand, within BWI76_RS05470 at 1148.462 kb on - strand, within BWI76_RS05470 at 1148.522 kb on + strand, within BWI76_RS05470 at 1148.528 kb on - strand, within BWI76_RS05470 at 1148.530 kb on - strand, within BWI76_RS05470 at 1148.565 kb on - strand, within BWI76_RS05470 at 1148.598 kb on + strand, within BWI76_RS05470 at 1148.606 kb on - strand, within BWI76_RS05470 at 1149.270 kb on + strand at 1150.067 kb on + strand, within BWI76_RS05480 at 1150.068 kb on + strand, within BWI76_RS05480 at 1150.076 kb on - strand, within BWI76_RS05480
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 1,145,997 - BWI76_RS05465 0.41 +0.3 1,146,396 - +0.6 1,146,490 + +0.1 1,146,982 - BWI76_RS05470 0.14 +1.0 1,147,030 + BWI76_RS05470 0.16 +0.8 1,147,132 - BWI76_RS05470 0.20 +1.2 1,147,231 + BWI76_RS05470 0.24 +0.9 1,147,317 + BWI76_RS05470 0.28 +0.8 1,147,347 - BWI76_RS05470 0.29 +1.1 1,147,395 - BWI76_RS05470 0.31 +1.0 1,147,421 - BWI76_RS05470 0.32 +0.2 1,147,428 + BWI76_RS05470 0.32 +1.3 1,147,945 - BWI76_RS05470 0.54 +1.4 1,148,018 + BWI76_RS05470 0.57 +0.9 1,148,123 + BWI76_RS05470 0.61 +0.2 1,148,147 - BWI76_RS05470 0.62 +0.0 1,148,429 + BWI76_RS05470 0.73 -1.8 1,148,462 - BWI76_RS05470 0.75 +1.1 1,148,462 - BWI76_RS05470 0.75 +0.3 1,148,522 + BWI76_RS05470 0.77 +1.2 1,148,528 - BWI76_RS05470 0.77 +0.9 1,148,530 - BWI76_RS05470 0.78 +1.6 1,148,565 - BWI76_RS05470 0.79 +1.1 1,148,598 + BWI76_RS05470 0.80 +0.2 1,148,606 - BWI76_RS05470 0.81 +1.0 1,149,270 + -0.5 1,150,067 + BWI76_RS05480 0.16 -0.3 1,150,068 + BWI76_RS05480 0.16 +1.2 1,150,076 - BWI76_RS05480 0.17 -0.6
Or see this region's nucleotide sequence