Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04170 and BWI76_RS04175 are separated by 98 nucleotides BWI76_RS04175 and BWI76_RS04180 overlap by 60 nucleotides BWI76_RS04180 and BWI76_RS04185 are separated by 125 nucleotides
BWI76_RS04170: BWI76_RS04170 - trifunctional nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase/transcriptional regulator NadR, at 858,057 to 859,289
_RS04170
BWI76_RS04175: BWI76_RS04175 - energy-dependent translational throttle protein EttA, at 859,388 to 861,055
_RS04175
BWI76_RS04180: BWI76_RS04180 - hypothetical protein, at 860,996 to 861,203
_RS04180
BWI76_RS04185: BWI76_RS04185 - murein transglycosylase, at 861,329 to 863,266
_RS04185
Position (kb) 859
860
861
862 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 858.530 kb on - strand, within BWI76_RS04170 at 858.594 kb on - strand, within BWI76_RS04170 at 858.772 kb on - strand, within BWI76_RS04170 at 858.893 kb on + strand, within BWI76_RS04170 at 859.069 kb on + strand, within BWI76_RS04170 at 859.127 kb on - strand, within BWI76_RS04170 at 859.167 kb on + strand at 859.208 kb on - strand at 859.257 kb on - strand at 859.318 kb on + strand at 859.507 kb on + strand at 859.589 kb on + strand, within BWI76_RS04175 at 859.713 kb on + strand, within BWI76_RS04175 at 859.742 kb on - strand, within BWI76_RS04175 at 860.196 kb on + strand, within BWI76_RS04175 at 861.021 kb on - strand at 861.062 kb on - strand, within BWI76_RS04180 at 861.082 kb on + strand, within BWI76_RS04180 at 861.118 kb on + strand, within BWI76_RS04180 at 861.126 kb on - strand, within BWI76_RS04180 at 861.131 kb on - strand, within BWI76_RS04180 at 861.133 kb on + strand, within BWI76_RS04180 at 861.176 kb on + strand, within BWI76_RS04180 at 861.293 kb on + strand at 861.530 kb on - strand, within BWI76_RS04185 at 861.687 kb on + strand, within BWI76_RS04185 at 861.768 kb on - strand, within BWI76_RS04185 at 861.778 kb on - strand, within BWI76_RS04185 at 861.835 kb on + strand, within BWI76_RS04185 at 861.897 kb on - strand, within BWI76_RS04185 at 862.016 kb on + strand, within BWI76_RS04185
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 858,530 - BWI76_RS04170 0.38 +0.6 858,594 - BWI76_RS04170 0.44 -0.8 858,772 - BWI76_RS04170 0.58 -0.1 858,893 + BWI76_RS04170 0.68 -1.4 859,069 + BWI76_RS04170 0.82 +1.2 859,127 - BWI76_RS04170 0.87 -2.0 859,167 + -1.1 859,208 - +0.1 859,257 - +1.2 859,318 + +3.5 859,507 + +1.0 859,589 + BWI76_RS04175 0.12 +1.1 859,713 + BWI76_RS04175 0.19 +1.1 859,742 - BWI76_RS04175 0.21 -0.6 860,196 + BWI76_RS04175 0.48 +0.8 861,021 - +1.5 861,062 - BWI76_RS04180 0.32 -1.3 861,082 + BWI76_RS04180 0.41 +0.9 861,118 + BWI76_RS04180 0.59 +0.8 861,126 - BWI76_RS04180 0.62 +0.5 861,131 - BWI76_RS04180 0.65 +0.6 861,133 + BWI76_RS04180 0.66 +0.3 861,176 + BWI76_RS04180 0.87 +0.5 861,293 + +0.8 861,530 - BWI76_RS04185 0.10 -0.5 861,687 + BWI76_RS04185 0.18 +1.0 861,768 - BWI76_RS04185 0.23 +0.1 861,778 - BWI76_RS04185 0.23 +0.1 861,835 + BWI76_RS04185 0.26 +0.6 861,897 - BWI76_RS04185 0.29 -1.8 862,016 + BWI76_RS04185 0.35 +0.6
Or see this region's nucleotide sequence