Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04000 and BWI76_RS04005 are separated by 2 nucleotides BWI76_RS04005 and BWI76_RS04010 are separated by 108 nucleotides BWI76_RS04010 and BWI76_RS04015 overlap by 10 nucleotides
BWI76_RS04000: BWI76_RS04000 - DNA replication protein DnaC, at 828,479 to 829,216
_RS04000
BWI76_RS04005: BWI76_RS04005 - primosomal protein DnaI, at 829,219 to 829,758
_RS04005
BWI76_RS04010: BWI76_RS04010 - hypothetical protein, at 829,867 to 830,340
_RS04010
BWI76_RS04015: BWI76_RS04015 - membrane protein, at 830,331 to 831,107
_RS04015
Position (kb) 829
830
831 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 829.789 kb on - strand at 829.807 kb on - strand at 829.888 kb on - strand at 829.991 kb on - strand, within BWI76_RS04010 at 830.052 kb on + strand, within BWI76_RS04010 at 830.106 kb on - strand, within BWI76_RS04010 at 830.152 kb on - strand, within BWI76_RS04010 at 830.228 kb on + strand, within BWI76_RS04010 at 830.236 kb on - strand, within BWI76_RS04010 at 830.297 kb on + strand at 830.297 kb on + strand at 830.305 kb on - strand at 830.305 kb on - strand at 830.383 kb on - strand at 830.566 kb on - strand, within BWI76_RS04015 at 830.566 kb on - strand, within BWI76_RS04015 at 830.625 kb on + strand, within BWI76_RS04015 at 830.763 kb on + strand, within BWI76_RS04015 at 830.778 kb on - strand, within BWI76_RS04015 at 830.926 kb on - strand, within BWI76_RS04015 at 830.934 kb on - strand, within BWI76_RS04015 at 830.950 kb on - strand, within BWI76_RS04015 at 830.973 kb on - strand, within BWI76_RS04015 at 831.008 kb on + strand, within BWI76_RS04015 at 831.063 kb on - strand at 831.117 kb on - strand at 831.197 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 829,789 - +0.4 829,807 - +0.7 829,888 - -0.8 829,991 - BWI76_RS04010 0.26 +1.9 830,052 + BWI76_RS04010 0.39 -0.7 830,106 - BWI76_RS04010 0.50 -0.0 830,152 - BWI76_RS04010 0.60 +1.1 830,228 + BWI76_RS04010 0.76 +1.7 830,236 - BWI76_RS04010 0.78 +0.8 830,297 + -0.1 830,297 + -1.6 830,305 - +0.8 830,305 - +0.6 830,383 - -1.7 830,566 - BWI76_RS04015 0.30 +0.8 830,566 - BWI76_RS04015 0.30 -0.2 830,625 + BWI76_RS04015 0.38 +0.4 830,763 + BWI76_RS04015 0.56 -1.3 830,778 - BWI76_RS04015 0.58 +0.6 830,926 - BWI76_RS04015 0.77 +0.4 830,934 - BWI76_RS04015 0.78 -0.3 830,950 - BWI76_RS04015 0.80 +3.2 830,973 - BWI76_RS04015 0.83 +1.2 831,008 + BWI76_RS04015 0.87 -1.9 831,063 - -1.8 831,117 - -0.3 831,197 - +0.9
Or see this region's nucleotide sequence