Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02680 and BWI76_RS02685 are separated by 4 nucleotides BWI76_RS02685 and BWI76_RS02690 are separated by 139 nucleotides BWI76_RS02690 and BWI76_RS02695 overlap by 69 nucleotides BWI76_RS02695 and BWI76_RS02700 are separated by 305 nucleotides
BWI76_RS02680: BWI76_RS02680 - EamA family transporter, at 544,058 to 545,011
_RS02680
BWI76_RS02685: BWI76_RS02685 - opacity-associated protein, at 545,016 to 545,675
_RS02685
BWI76_RS02690: BWI76_RS02690 - hypothetical protein, at 545,815 to 546,012
_RS02690
BWI76_RS02695: BWI76_RS02695 - peptidyl-prolyl cis-trans isomerase, at 545,944 to 546,564
_RS02695
BWI76_RS02700: BWI76_RS02700 - D-serine/D-alanine/glycine transporter, at 546,870 to 548,273
_RS02700
Position (kb) 545
546
547 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 545.124 kb on + strand, within BWI76_RS02685 at 545.147 kb on + strand, within BWI76_RS02685 at 545.150 kb on - strand, within BWI76_RS02685 at 545.207 kb on + strand, within BWI76_RS02685 at 545.224 kb on + strand, within BWI76_RS02685 at 545.265 kb on - strand, within BWI76_RS02685 at 545.499 kb on + strand, within BWI76_RS02685 at 545.507 kb on - strand, within BWI76_RS02685 at 545.713 kb on + strand at 545.776 kb on - strand at 545.776 kb on - strand at 545.782 kb on + strand at 545.867 kb on + strand, within BWI76_RS02690 at 545.966 kb on + strand at 546.155 kb on - strand, within BWI76_RS02695 at 546.318 kb on + strand, within BWI76_RS02695 at 546.480 kb on + strand, within BWI76_RS02695 at 546.480 kb on + strand, within BWI76_RS02695 at 546.519 kb on + strand at 546.597 kb on - strand at 546.621 kb on - strand at 546.708 kb on + strand at 546.792 kb on + strand at 546.804 kb on + strand at 546.806 kb on - strand at 546.841 kb on + strand at 546.879 kb on - strand at 546.889 kb on - strand at 546.889 kb on - strand at 546.905 kb on + strand at 546.913 kb on - strand at 546.982 kb on + strand at 547.000 kb on - strand at 547.250 kb on + strand, within BWI76_RS02700 at 547.258 kb on - strand, within BWI76_RS02700 at 547.541 kb on - strand, within BWI76_RS02700 at 547.549 kb on + strand, within BWI76_RS02700 at 547.559 kb on + strand, within BWI76_RS02700
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 545,124 + BWI76_RS02685 0.16 -0.4 545,147 + BWI76_RS02685 0.20 +0.2 545,150 - BWI76_RS02685 0.20 -0.2 545,207 + BWI76_RS02685 0.29 +0.3 545,224 + BWI76_RS02685 0.32 -0.4 545,265 - BWI76_RS02685 0.38 +1.1 545,499 + BWI76_RS02685 0.73 -1.1 545,507 - BWI76_RS02685 0.74 +0.9 545,713 + -1.3 545,776 - +0.5 545,776 - +2.7 545,782 + -0.4 545,867 + BWI76_RS02690 0.26 +0.6 545,966 + -1.6 546,155 - BWI76_RS02695 0.34 +1.5 546,318 + BWI76_RS02695 0.60 +0.2 546,480 + BWI76_RS02695 0.86 +1.1 546,480 + BWI76_RS02695 0.86 +0.4 546,519 + +1.0 546,597 - +1.6 546,621 - +0.1 546,708 + +2.2 546,792 + -0.5 546,804 + -0.4 546,806 - +0.8 546,841 + +3.4 546,879 - -1.1 546,889 - -0.2 546,889 - -0.4 546,905 + +0.3 546,913 - +0.2 546,982 + +1.0 547,000 - -0.6 547,250 + BWI76_RS02700 0.27 +0.4 547,258 - BWI76_RS02700 0.28 -2.0 547,541 - BWI76_RS02700 0.48 +0.4 547,549 + BWI76_RS02700 0.48 +0.6 547,559 + BWI76_RS02700 0.49 +0.6
Or see this region's nucleotide sequence