Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02620 and BWI76_RS02625 are separated by 12 nucleotides BWI76_RS02625 and BWI76_RS02630 are separated by 9 nucleotides BWI76_RS02630 and BWI76_RS02635 are separated by 13 nucleotides BWI76_RS02635 and BWI76_RS02640 are separated by 9 nucleotides
BWI76_RS02620: BWI76_RS02620 - PTS ascorbate transporter subunit IIC, at 536,057 to 537,457
_RS02620
BWI76_RS02625: BWI76_RS02625 - PTS ascorbate transporter subunit IIB, at 537,470 to 537,775
_RS02625
BWI76_RS02630: BWI76_RS02630 - PTS ascorbate transporter subunit IIA, at 537,785 to 538,252
_RS02630
BWI76_RS02635: BWI76_RS02635 - 3-keto-L-gulonate-6-phosphate decarboxylase, at 538,266 to 538,916
_RS02635
BWI76_RS02640: BWI76_RS02640 - xylulose 5-phosphate 3-epimerase, at 538,926 to 539,780
_RS02640
Position (kb) 537
538
539 Strain fitness (log2 ratio) -2
-1
0
1 at 536.827 kb on + strand, within BWI76_RS02620 at 536.836 kb on + strand, within BWI76_RS02620 at 536.844 kb on - strand, within BWI76_RS02620 at 536.856 kb on + strand, within BWI76_RS02620 at 536.905 kb on + strand, within BWI76_RS02620 at 537.035 kb on + strand, within BWI76_RS02620 at 537.115 kb on - strand, within BWI76_RS02620 at 537.241 kb on + strand, within BWI76_RS02620 at 537.331 kb on - strand at 537.376 kb on + strand at 537.397 kb on + strand at 537.487 kb on + strand at 537.491 kb on + strand at 537.529 kb on + strand, within BWI76_RS02625 at 537.583 kb on - strand, within BWI76_RS02625 at 537.590 kb on - strand, within BWI76_RS02625 at 537.644 kb on - strand, within BWI76_RS02625 at 537.824 kb on - strand at 537.868 kb on + strand, within BWI76_RS02630 at 537.908 kb on + strand, within BWI76_RS02630 at 537.941 kb on - strand, within BWI76_RS02630 at 538.020 kb on + strand, within BWI76_RS02630 at 538.154 kb on - strand, within BWI76_RS02630 at 538.156 kb on + strand, within BWI76_RS02630 at 538.168 kb on + strand, within BWI76_RS02630 at 538.334 kb on + strand, within BWI76_RS02635 at 538.342 kb on - strand, within BWI76_RS02635 at 538.538 kb on + strand, within BWI76_RS02635 at 538.707 kb on - strand, within BWI76_RS02635 at 538.731 kb on + strand, within BWI76_RS02635 at 538.810 kb on - strand, within BWI76_RS02635 at 538.883 kb on + strand at 538.891 kb on - strand at 538.904 kb on + strand at 538.980 kb on - strand at 539.103 kb on - strand, within BWI76_RS02640 at 539.103 kb on - strand, within BWI76_RS02640 at 539.103 kb on - strand, within BWI76_RS02640 at 539.113 kb on - strand, within BWI76_RS02640 at 539.187 kb on - strand, within BWI76_RS02640
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 536,827 + BWI76_RS02620 0.55 -0.6 536,836 + BWI76_RS02620 0.56 +0.7 536,844 - BWI76_RS02620 0.56 -0.6 536,856 + BWI76_RS02620 0.57 +0.2 536,905 + BWI76_RS02620 0.61 +0.2 537,035 + BWI76_RS02620 0.70 +0.4 537,115 - BWI76_RS02620 0.76 +0.2 537,241 + BWI76_RS02620 0.85 +1.0 537,331 - -0.6 537,376 + +0.2 537,397 + -1.2 537,487 + -0.2 537,491 + +0.2 537,529 + BWI76_RS02625 0.19 +0.7 537,583 - BWI76_RS02625 0.37 +0.8 537,590 - BWI76_RS02625 0.39 +0.4 537,644 - BWI76_RS02625 0.57 -2.1 537,824 - +1.4 537,868 + BWI76_RS02630 0.18 +0.6 537,908 + BWI76_RS02630 0.26 +0.7 537,941 - BWI76_RS02630 0.33 +0.4 538,020 + BWI76_RS02630 0.50 -1.6 538,154 - BWI76_RS02630 0.79 +0.3 538,156 + BWI76_RS02630 0.79 +1.4 538,168 + BWI76_RS02630 0.82 +1.3 538,334 + BWI76_RS02635 0.10 -1.7 538,342 - BWI76_RS02635 0.12 -0.7 538,538 + BWI76_RS02635 0.42 -0.9 538,707 - BWI76_RS02635 0.68 -1.4 538,731 + BWI76_RS02635 0.71 -0.4 538,810 - BWI76_RS02635 0.84 -2.7 538,883 + -0.1 538,891 - +0.2 538,904 + -0.2 538,980 - -0.6 539,103 - BWI76_RS02640 0.21 +0.4 539,103 - BWI76_RS02640 0.21 -0.1 539,103 - BWI76_RS02640 0.21 +0.0 539,113 - BWI76_RS02640 0.22 -1.7 539,187 - BWI76_RS02640 0.31 -1.1
Or see this region's nucleotide sequence