Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00815 and BWI76_RS00820 are separated by 56 nucleotides BWI76_RS00820 and BWI76_RS00825 are separated by 205 nucleotides BWI76_RS00825 and BWI76_RS00830 are separated by 151 nucleotides BWI76_RS00830 and BWI76_RS00835 are separated by 97 nucleotides
BWI76_RS00815: BWI76_RS00815 - glycerol dehydrogenase, at 166,891 to 167,994
_RS00815
BWI76_RS00820: BWI76_RS00820 - fructose-6-phosphate aldolase, at 168,051 to 168,713
_RS00820
BWI76_RS00825: BWI76_RS00825 - AraC family transcriptional regulator, at 168,919 to 169,782
_RS00825
BWI76_RS00830: BWI76_RS00830 - metal-binding protein ZinT, at 169,934 to 170,581
_RS00830
BWI76_RS00835: BWI76_RS00835 - phosphoenolpyruvate carboxylase, at 170,679 to 173,330
_RS00835
Position (kb) 168
169
170 Strain fitness (log2 ratio) -2
-1
0
1
2 at 168.079 kb on + strand at 168.079 kb on + strand at 168.096 kb on + strand at 168.189 kb on + strand, within BWI76_RS00820 at 168.297 kb on + strand, within BWI76_RS00820 at 168.305 kb on - strand, within BWI76_RS00820 at 168.384 kb on - strand, within BWI76_RS00820 at 168.395 kb on + strand, within BWI76_RS00820 at 168.481 kb on + strand, within BWI76_RS00820 at 168.590 kb on - strand, within BWI76_RS00820 at 168.742 kb on + strand at 168.848 kb on + strand at 168.978 kb on - strand at 169.099 kb on - strand, within BWI76_RS00825 at 169.128 kb on + strand, within BWI76_RS00825 at 169.137 kb on + strand, within BWI76_RS00825 at 169.285 kb on + strand, within BWI76_RS00825 at 169.293 kb on - strand, within BWI76_RS00825 at 169.346 kb on - strand, within BWI76_RS00825 at 169.495 kb on - strand, within BWI76_RS00825 at 169.509 kb on + strand, within BWI76_RS00825 at 169.529 kb on - strand, within BWI76_RS00825 at 169.610 kb on + strand, within BWI76_RS00825 at 169.625 kb on + strand, within BWI76_RS00825 at 169.825 kb on + strand at 169.843 kb on - strand at 169.919 kb on + strand at 169.927 kb on - strand at 169.982 kb on - strand at 170.030 kb on - strand, within BWI76_RS00830 at 170.082 kb on - strand, within BWI76_RS00830 at 170.235 kb on - strand, within BWI76_RS00830 at 170.241 kb on + strand, within BWI76_RS00830 at 170.251 kb on + strand, within BWI76_RS00830 at 170.552 kb on - strand at 170.664 kb on - strand at 170.694 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 168,079 + +0.0 168,079 + -0.1 168,096 + -0.7 168,189 + BWI76_RS00820 0.21 +0.0 168,297 + BWI76_RS00820 0.37 +0.2 168,305 - BWI76_RS00820 0.38 +0.4 168,384 - BWI76_RS00820 0.50 +0.3 168,395 + BWI76_RS00820 0.52 +0.7 168,481 + BWI76_RS00820 0.65 +0.0 168,590 - BWI76_RS00820 0.81 -0.4 168,742 + +0.8 168,848 + +0.7 168,978 - -1.3 169,099 - BWI76_RS00825 0.21 +0.8 169,128 + BWI76_RS00825 0.24 +0.0 169,137 + BWI76_RS00825 0.25 +1.3 169,285 + BWI76_RS00825 0.42 +1.2 169,293 - BWI76_RS00825 0.43 +1.3 169,346 - BWI76_RS00825 0.49 +0.2 169,495 - BWI76_RS00825 0.67 -1.8 169,509 + BWI76_RS00825 0.68 +0.8 169,529 - BWI76_RS00825 0.71 +2.3 169,610 + BWI76_RS00825 0.80 +0.3 169,625 + BWI76_RS00825 0.82 +1.1 169,825 + +0.7 169,843 - +0.2 169,919 + +0.4 169,927 - +1.9 169,982 - -0.5 170,030 - BWI76_RS00830 0.15 -1.3 170,082 - BWI76_RS00830 0.23 +0.4 170,235 - BWI76_RS00830 0.46 -1.0 170,241 + BWI76_RS00830 0.47 +0.2 170,251 + BWI76_RS00830 0.49 +0.2 170,552 - -0.1 170,664 - -0.1 170,694 - +1.7
Or see this region's nucleotide sequence