Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00785 and BWI76_RS00790 are separated by 2 nucleotides BWI76_RS00790 and BWI76_RS00795 are separated by 39 nucleotides BWI76_RS00795 and BWI76_RS00800 are separated by 91 nucleotides
BWI76_RS00785: BWI76_RS00785 - cystathionine gamma-synthase, at 159,813 to 160,973
_RS00785
BWI76_RS00790: BWI76_RS00790 - bifunctional aspartokinase II/homoserine dehydrogenase II, at 160,976 to 163,408
_RS00790
BWI76_RS00795: BWI76_RS00795 - carboxymuconolactone decarboxylase, at 163,448 to 163,819
_RS00795
BWI76_RS00800: BWI76_RS00800 - LysR family transcriptional regulator, at 163,911 to 164,816
_RS00800
Position (kb) 160
161
162
163
164 Strain fitness (log2 ratio) -1
0
1
2 at 160.023 kb on + strand, within BWI76_RS00785 at 160.223 kb on + strand, within BWI76_RS00785 at 160.262 kb on + strand, within BWI76_RS00785 at 160.277 kb on - strand, within BWI76_RS00785 at 160.337 kb on - strand, within BWI76_RS00785 at 160.423 kb on + strand at 160.526 kb on - strand, within BWI76_RS00785 at 160.546 kb on + strand, within BWI76_RS00785 at 160.575 kb on + strand, within BWI76_RS00785 at 160.575 kb on + strand, within BWI76_RS00785 at 160.607 kb on + strand, within BWI76_RS00785 at 160.770 kb on - strand, within BWI76_RS00785 at 160.906 kb on + strand at 160.906 kb on + strand at 160.906 kb on + strand at 161.001 kb on - strand at 161.015 kb on - strand at 161.424 kb on + strand, within BWI76_RS00790 at 161.587 kb on + strand, within BWI76_RS00790 at 161.635 kb on + strand, within BWI76_RS00790 at 161.852 kb on - strand, within BWI76_RS00790 at 161.866 kb on + strand, within BWI76_RS00790 at 162.077 kb on - strand, within BWI76_RS00790 at 162.134 kb on - strand, within BWI76_RS00790 at 162.769 kb on + strand, within BWI76_RS00790 at 162.812 kb on - strand, within BWI76_RS00790 at 162.843 kb on + strand, within BWI76_RS00790 at 162.911 kb on - strand, within BWI76_RS00790 at 163.095 kb on + strand, within BWI76_RS00790 at 163.183 kb on + strand at 163.191 kb on - strand at 163.379 kb on + strand at 163.613 kb on - strand, within BWI76_RS00795 at 163.650 kb on - strand, within BWI76_RS00795 at 163.740 kb on - strand, within BWI76_RS00795 at 163.778 kb on + strand, within BWI76_RS00795 at 163.799 kb on + strand at 163.822 kb on - strand at 163.839 kb on - strand at 163.866 kb on + strand at 163.889 kb on - strand at 164.060 kb on + strand at 164.154 kb on + strand, within BWI76_RS00800 at 164.233 kb on + strand, within BWI76_RS00800 at 164.241 kb on - strand, within BWI76_RS00800 at 164.241 kb on - strand, within BWI76_RS00800
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 160,023 + BWI76_RS00785 0.18 +0.1 160,223 + BWI76_RS00785 0.35 -1.7 160,262 + BWI76_RS00785 0.39 +0.1 160,277 - BWI76_RS00785 0.40 +0.6 160,337 - BWI76_RS00785 0.45 +2.2 160,423 + -0.7 160,526 - BWI76_RS00785 0.61 +0.7 160,546 + BWI76_RS00785 0.63 -1.1 160,575 + BWI76_RS00785 0.66 +1.6 160,575 + BWI76_RS00785 0.66 +0.6 160,607 + BWI76_RS00785 0.68 +1.3 160,770 - BWI76_RS00785 0.82 +1.6 160,906 + +0.8 160,906 + +0.5 160,906 + +1.3 161,001 - +1.6 161,015 - +2.6 161,424 + BWI76_RS00790 0.18 +1.9 161,587 + BWI76_RS00790 0.25 +0.7 161,635 + BWI76_RS00790 0.27 +2.4 161,852 - BWI76_RS00790 0.36 +1.4 161,866 + BWI76_RS00790 0.37 +1.4 162,077 - BWI76_RS00790 0.45 +1.9 162,134 - BWI76_RS00790 0.48 +2.0 162,769 + BWI76_RS00790 0.74 +1.8 162,812 - BWI76_RS00790 0.75 -0.9 162,843 + BWI76_RS00790 0.77 +1.0 162,911 - BWI76_RS00790 0.80 +1.5 163,095 + BWI76_RS00790 0.87 +1.2 163,183 + -0.7 163,191 - +1.1 163,379 + +1.3 163,613 - BWI76_RS00795 0.44 -0.3 163,650 - BWI76_RS00795 0.54 -0.5 163,740 - BWI76_RS00795 0.78 +1.3 163,778 + BWI76_RS00795 0.89 -0.6 163,799 + +0.0 163,822 - +0.1 163,839 - +0.4 163,866 + -0.3 163,889 - +0.5 164,060 + -1.0 164,154 + BWI76_RS00800 0.27 +0.3 164,233 + BWI76_RS00800 0.36 -0.9 164,241 - BWI76_RS00800 0.36 -0.2 164,241 - BWI76_RS00800 0.36 +0.4
Or see this region's nucleotide sequence