Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00750 and BWI76_RS00755 are separated by 9 nucleotides BWI76_RS00755 and BWI76_RS00760 are separated by 91 nucleotides BWI76_RS00760 and BWI76_RS00765 are separated by 91 nucleotides
BWI76_RS00750: BWI76_RS00750 - ATP-dependent protease ATP-binding subunit HslU, at 152,271 to 153,605
_RS00750
BWI76_RS00755: BWI76_RS00755 - ATP-dependent protease subunit HslV, at 153,615 to 154,145
_RS00755
BWI76_RS00760: BWI76_RS00760 - cell division protein FtsN, at 154,237 to 155,211
_RS00760
BWI76_RS00765: BWI76_RS00765 - DNA-binding transcriptional regulator CytR, at 155,303 to 156,331
_RS00765
Position (kb) 154
155
156 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 153.484 kb on - strand at 153.489 kb on - strand at 153.823 kb on + strand, within BWI76_RS00755 at 153.967 kb on + strand, within BWI76_RS00755 at 154.069 kb on + strand, within BWI76_RS00755 at 154.077 kb on - strand, within BWI76_RS00755 at 154.082 kb on - strand, within BWI76_RS00755 at 154.114 kb on - strand at 154.369 kb on - strand, within BWI76_RS00760 at 154.417 kb on - strand, within BWI76_RS00760 at 154.532 kb on + strand, within BWI76_RS00760 at 154.540 kb on - strand, within BWI76_RS00760 at 154.540 kb on - strand, within BWI76_RS00760 at 154.540 kb on - strand, within BWI76_RS00760 at 154.565 kb on - strand, within BWI76_RS00760 at 155.382 kb on - strand at 155.397 kb on + strand at 155.521 kb on + strand, within BWI76_RS00765 at 155.521 kb on + strand, within BWI76_RS00765 at 155.973 kb on - strand, within BWI76_RS00765 at 156.005 kb on + strand, within BWI76_RS00765 at 156.005 kb on + strand, within BWI76_RS00765 at 156.005 kb on + strand, within BWI76_RS00765 at 156.041 kb on + strand, within BWI76_RS00765 at 156.059 kb on + strand, within BWI76_RS00765
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 153,484 - +1.7 153,489 - -1.0 153,823 + BWI76_RS00755 0.39 -3.2 153,967 + BWI76_RS00755 0.66 +0.4 154,069 + BWI76_RS00755 0.85 -1.6 154,077 - BWI76_RS00755 0.87 +0.2 154,082 - BWI76_RS00755 0.88 -0.9 154,114 - -1.0 154,369 - BWI76_RS00760 0.14 -2.0 154,417 - BWI76_RS00760 0.18 +0.9 154,532 + BWI76_RS00760 0.30 +0.1 154,540 - BWI76_RS00760 0.31 +0.2 154,540 - BWI76_RS00760 0.31 +1.1 154,540 - BWI76_RS00760 0.31 +1.0 154,565 - BWI76_RS00760 0.34 -0.7 155,382 - -0.8 155,397 + -1.6 155,521 + BWI76_RS00765 0.21 -0.5 155,521 + BWI76_RS00765 0.21 -0.5 155,973 - BWI76_RS00765 0.65 -0.6 156,005 + BWI76_RS00765 0.68 -3.2 156,005 + BWI76_RS00765 0.68 -0.7 156,005 + BWI76_RS00765 0.68 +0.7 156,041 + BWI76_RS00765 0.72 -1.8 156,059 + BWI76_RS00765 0.73 +0.4
Or see this region's nucleotide sequence