Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26105 and BWI76_RS26110 overlap by 11 nucleotides BWI76_RS26110 and BWI76_RS26115 overlap by 11 nucleotides BWI76_RS26115 and BWI76_RS26120 overlap by 4 nucleotides BWI76_RS26120 and BWI76_RS26125 are separated by 117 nucleotides
BWI76_RS26105: BWI76_RS26105 - hypothetical protein, at 5,393,267 to 5,393,674
_RS26105
BWI76_RS26110: BWI76_RS26110 - hypothetical protein, at 5,393,664 to 5,394,104
_RS26110
BWI76_RS26115: BWI76_RS26115 - hypothetical protein, at 5,394,094 to 5,394,627
_RS26115
BWI76_RS26120: BWI76_RS26120 - hypothetical protein, at 5,394,624 to 5,395,415
_RS26120
BWI76_RS26125: BWI76_RS26125 - carboxypeptidase/penicillin-binding protein 1A, at 5,395,533 to 5,398,091
_RS26125
Position (kb) 5394
5395
5396 Strain fitness (log2 ratio) -2
-1
0
1 at 5393.967 kb on - strand, within BWI76_RS26110 at 5394.013 kb on - strand, within BWI76_RS26110 at 5394.016 kb on + strand, within BWI76_RS26110 at 5394.025 kb on + strand, within BWI76_RS26110 at 5394.175 kb on - strand, within BWI76_RS26115 at 5394.258 kb on + strand, within BWI76_RS26115 at 5394.288 kb on + strand, within BWI76_RS26115 at 5394.440 kb on + strand, within BWI76_RS26115 at 5394.448 kb on - strand, within BWI76_RS26115 at 5394.448 kb on - strand, within BWI76_RS26115 at 5394.448 kb on - strand, within BWI76_RS26115 at 5394.622 kb on + strand at 5394.664 kb on - strand at 5394.887 kb on + strand, within BWI76_RS26120 at 5395.067 kb on + strand, within BWI76_RS26120 at 5395.315 kb on - strand, within BWI76_RS26120 at 5395.322 kb on + strand, within BWI76_RS26120 at 5395.471 kb on + strand at 5395.580 kb on + strand at 5395.580 kb on + strand at 5395.588 kb on - strand at 5395.636 kb on - strand at 5395.646 kb on + strand at 5395.654 kb on + strand at 5395.734 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 5,393,967 - BWI76_RS26110 0.69 -0.2 5,394,013 - BWI76_RS26110 0.79 +1.0 5,394,016 + BWI76_RS26110 0.80 +0.3 5,394,025 + BWI76_RS26110 0.82 -0.1 5,394,175 - BWI76_RS26115 0.15 +0.8 5,394,258 + BWI76_RS26115 0.31 +1.4 5,394,288 + BWI76_RS26115 0.36 +0.4 5,394,440 + BWI76_RS26115 0.65 +1.6 5,394,448 - BWI76_RS26115 0.66 +1.1 5,394,448 - BWI76_RS26115 0.66 -0.7 5,394,448 - BWI76_RS26115 0.66 -0.7 5,394,622 + -0.2 5,394,664 - -0.9 5,394,887 + BWI76_RS26120 0.33 -2.2 5,395,067 + BWI76_RS26120 0.56 -1.0 5,395,315 - BWI76_RS26120 0.87 -0.2 5,395,322 + BWI76_RS26120 0.88 -0.5 5,395,471 + +1.4 5,395,580 + -0.2 5,395,580 + -0.4 5,395,588 - -1.9 5,395,636 - +0.9 5,395,646 + +0.3 5,395,654 + +0.2 5,395,734 - -0.6
Or see this region's nucleotide sequence