Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24895 and BWI76_RS24900 are separated by 110 nucleotides BWI76_RS24900 and BWI76_RS24905 are separated by 64 nucleotides BWI76_RS24905 and BWI76_RS24910 are separated by 4 nucleotides BWI76_RS24910 and BWI76_RS24915 are separated by 63 nucleotides
BWI76_RS24895: BWI76_RS24895 - galactitol-1-phosphate 5-dehydrogenase, at 5,187,513 to 5,188,556
_RS24895
BWI76_RS24900: BWI76_RS24900 - DeoR family transcriptional regulator, at 5,188,667 to 5,189,440
_RS24900
BWI76_RS24905: BWI76_RS24905 - filamentation induced by cAMP protein fic, at 5,189,505 to 5,190,506
_RS24905
BWI76_RS24910: BWI76_RS24910 - rRNA (cytidine-2'-O-)-methyltransferase, at 5,190,511 to 5,191,374
_RS24910
BWI76_RS24915: BWI76_RS24915 - penicillin-binding protein activator, at 5,191,438 to 5,193,519
_RS24915
Position (kb) 5189
5190
5191 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5188.674 kb on + strand at 5188.833 kb on + strand, within BWI76_RS24900 at 5188.991 kb on + strand, within BWI76_RS24900 at 5189.060 kb on + strand, within BWI76_RS24900 at 5189.135 kb on + strand, within BWI76_RS24900 at 5189.410 kb on - strand at 5189.450 kb on - strand at 5189.470 kb on + strand at 5189.620 kb on + strand, within BWI76_RS24905 at 5189.627 kb on - strand, within BWI76_RS24905 at 5189.830 kb on - strand, within BWI76_RS24905 at 5189.844 kb on - strand, within BWI76_RS24905 at 5189.921 kb on + strand, within BWI76_RS24905 at 5189.976 kb on + strand, within BWI76_RS24905 at 5189.994 kb on + strand, within BWI76_RS24905 at 5190.067 kb on + strand, within BWI76_RS24905 at 5190.123 kb on + strand, within BWI76_RS24905 at 5190.300 kb on - strand, within BWI76_RS24905 at 5190.344 kb on - strand, within BWI76_RS24905 at 5190.344 kb on - strand, within BWI76_RS24905 at 5190.406 kb on + strand at 5190.410 kb on - strand at 5190.468 kb on + strand at 5190.481 kb on - strand at 5190.508 kb on + strand at 5190.517 kb on - strand at 5190.523 kb on - strand at 5190.536 kb on + strand at 5190.536 kb on + strand at 5190.536 kb on + strand at 5190.544 kb on - strand at 5190.544 kb on - strand at 5190.699 kb on - strand, within BWI76_RS24910 at 5190.911 kb on - strand, within BWI76_RS24910 at 5191.009 kb on + strand, within BWI76_RS24910 at 5191.010 kb on + strand, within BWI76_RS24910 at 5191.125 kb on - strand, within BWI76_RS24910 at 5191.125 kb on - strand, within BWI76_RS24910 at 5191.127 kb on + strand, within BWI76_RS24910 at 5191.135 kb on - strand, within BWI76_RS24910 at 5191.275 kb on + strand, within BWI76_RS24910 at 5191.283 kb on - strand, within BWI76_RS24910 at 5191.319 kb on - strand at 5191.336 kb on - strand at 5191.336 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 5,188,674 + -1.6 5,188,833 + BWI76_RS24900 0.21 -0.6 5,188,991 + BWI76_RS24900 0.42 +1.4 5,189,060 + BWI76_RS24900 0.51 -1.4 5,189,135 + BWI76_RS24900 0.60 +1.4 5,189,410 - +1.4 5,189,450 - +0.3 5,189,470 + -3.0 5,189,620 + BWI76_RS24905 0.11 -0.3 5,189,627 - BWI76_RS24905 0.12 +0.6 5,189,830 - BWI76_RS24905 0.32 -1.7 5,189,844 - BWI76_RS24905 0.34 -3.6 5,189,921 + BWI76_RS24905 0.42 +0.9 5,189,976 + BWI76_RS24905 0.47 -1.4 5,189,994 + BWI76_RS24905 0.49 +0.3 5,190,067 + BWI76_RS24905 0.56 -0.9 5,190,123 + BWI76_RS24905 0.62 -0.2 5,190,300 - BWI76_RS24905 0.79 +0.7 5,190,344 - BWI76_RS24905 0.84 -2.0 5,190,344 - BWI76_RS24905 0.84 +0.7 5,190,406 + +0.8 5,190,410 - +0.2 5,190,468 + +0.5 5,190,481 - -0.8 5,190,508 + -0.4 5,190,517 - +0.1 5,190,523 - -0.4 5,190,536 + -1.1 5,190,536 + -2.2 5,190,536 + -0.6 5,190,544 - -0.6 5,190,544 - -1.9 5,190,699 - BWI76_RS24910 0.22 -0.2 5,190,911 - BWI76_RS24910 0.46 -3.0 5,191,009 + BWI76_RS24910 0.58 -0.4 5,191,010 + BWI76_RS24910 0.58 -0.6 5,191,125 - BWI76_RS24910 0.71 -0.9 5,191,125 - BWI76_RS24910 0.71 -1.9 5,191,127 + BWI76_RS24910 0.71 -2.3 5,191,135 - BWI76_RS24910 0.72 +1.4 5,191,275 + BWI76_RS24910 0.88 +0.4 5,191,283 - BWI76_RS24910 0.89 -0.3 5,191,319 - +1.0 5,191,336 - +0.0 5,191,336 - -0.6
Or see this region's nucleotide sequence