Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20440

Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20435 and BWI76_RS20440 are separated by 37 nucleotidesBWI76_RS20440 and BWI76_RS20445 are separated by 167 nucleotidesBWI76_RS20445 and BWI76_RS20450 are separated by 20 nucleotides BWI76_RS20435: BWI76_RS20435 - chorismate synthase, at 4,277,326 to 4,278,411 _RS20435 BWI76_RS20440: BWI76_RS20440 - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 4,278,449 to 4,279,381 _RS20440 BWI76_RS20445: BWI76_RS20445 - endonuclease SmrB, at 4,279,549 to 4,280,100 _RS20445 BWI76_RS20450: BWI76_RS20450 - phosphohistidine phosphatase, at 4,280,121 to 4,280,606 _RS20450 Position (kb) 4278 4279 4280Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4277.528 kb on + strand, within BWI76_RS20435at 4278.248 kb on - strand, within BWI76_RS20435at 4278.348 kb on - strandat 4278.588 kb on - strand, within BWI76_RS20440at 4278.639 kb on + strand, within BWI76_RS20440at 4278.867 kb on + strand, within BWI76_RS20440at 4278.867 kb on + strand, within BWI76_RS20440at 4279.395 kb on - strandat 4279.396 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
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4,277,528 + BWI76_RS20435 0.19 +1.3
4,278,248 - BWI76_RS20435 0.85 -0.3
4,278,348 - -2.5
4,278,588 - BWI76_RS20440 0.15 -2.3
4,278,639 + BWI76_RS20440 0.20 -3.5
4,278,867 + BWI76_RS20440 0.45 -1.0
4,278,867 + BWI76_RS20440 0.45 +3.3
4,279,395 - -0.1
4,279,396 - +0.7

Or see this region's nucleotide sequence