Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19135 and BWI76_RS19140 are separated by 127 nucleotides BWI76_RS19140 and BWI76_RS19145 are separated by 280 nucleotides BWI76_RS19145 and BWI76_RS19150 overlap by 4 nucleotides
BWI76_RS19135: BWI76_RS19135 - molecular chaperone, at 3,977,957 to 3,979,309
_RS19135
BWI76_RS19140: BWI76_RS19140 - citrate (Si)-synthase, at 3,979,437 to 3,980,723
_RS19140
BWI76_RS19145: BWI76_RS19145 - formate dehydrogenase cytochrome b556 subunit, at 3,981,004 to 3,981,633
_RS19145
BWI76_RS19150: BWI76_RS19150 - formate dehydrogenase subunit beta, at 3,981,630 to 3,982,532
_RS19150
Position (kb)
3979
3980
3981 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3978.508 kb on + strand, within BWI76_RS19135 at 3978.717 kb on + strand, within BWI76_RS19135 at 3978.946 kb on - strand, within BWI76_RS19135 at 3978.993 kb on - strand, within BWI76_RS19135 at 3979.128 kb on - strand, within BWI76_RS19135 at 3979.230 kb on - strand at 3979.335 kb on + strand at 3979.363 kb on + strand at 3979.390 kb on + strand at 3979.391 kb on - strand at 3979.609 kb on + strand, within BWI76_RS19140 at 3979.718 kb on - strand, within BWI76_RS19140 at 3979.790 kb on + strand, within BWI76_RS19140 at 3979.890 kb on - strand, within BWI76_RS19140 at 3980.306 kb on + strand, within BWI76_RS19140 at 3980.319 kb on + strand, within BWI76_RS19140 at 3980.319 kb on + strand, within BWI76_RS19140 at 3980.327 kb on - strand, within BWI76_RS19140 at 3980.484 kb on - strand, within BWI76_RS19140 at 3980.662 kb on + strand at 3981.070 kb on - strand, within BWI76_RS19145
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 3,978,508 + BWI76_RS19135 0.41 +0.2 3,978,717 + BWI76_RS19135 0.56 +0.1 3,978,946 - BWI76_RS19135 0.73 +0.5 3,978,993 - BWI76_RS19135 0.77 -0.7 3,979,128 - BWI76_RS19135 0.87 +0.3 3,979,230 - -0.0 3,979,335 + -1.1 3,979,363 + -1.9 3,979,390 + -0.2 3,979,391 - -0.6 3,979,609 + BWI76_RS19140 0.13 -0.2 3,979,718 - BWI76_RS19140 0.22 +0.4 3,979,790 + BWI76_RS19140 0.27 -1.3 3,979,890 - BWI76_RS19140 0.35 -0.4 3,980,306 + BWI76_RS19140 0.68 -2.5 3,980,319 + BWI76_RS19140 0.69 -1.8 3,980,319 + BWI76_RS19140 0.69 -0.1 3,980,327 - BWI76_RS19140 0.69 -0.5 3,980,484 - BWI76_RS19140 0.81 -3.6 3,980,662 + +0.1 3,981,070 - BWI76_RS19145 0.10 +0.5
Or see this region's nucleotide sequence