Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15435 and BWI76_RS15440 are separated by 25 nucleotides BWI76_RS15440 and BWI76_RS15445 are separated by 380 nucleotides BWI76_RS15445 and BWI76_RS15450 are separated by 62 nucleotides BWI76_RS15450 and BWI76_RS15455 are separated by 163 nucleotides BWI76_RS15455 and BWI76_RS15460 are separated by 114 nucleotides
BWI76_RS15435: BWI76_RS15435 - hypothetical protein, at 3,236,108 to 3,236,371
_RS15435
BWI76_RS15440: BWI76_RS15440 - hypothetical protein, at 3,236,397 to 3,236,651
_RS15440
BWI76_RS15445: BWI76_RS15445 - hypothetical protein, at 3,237,032 to 3,237,304
_RS15445
BWI76_RS15450: BWI76_RS15450 - hypothetical protein, at 3,237,367 to 3,237,678
_RS15450
BWI76_RS15455: BWI76_RS15455 - nucleoprotein/polynucleotide-associated enzyme, at 3,237,842 to 3,238,381
_RS15455
BWI76_RS15460: BWI76_RS15460 - cold-shock protein, at 3,238,496 to 3,238,972
_RS15460
Position (kb)
3237
3238 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3236.410 kb on + strand at 3236.567 kb on - strand, within BWI76_RS15440 at 3236.581 kb on + strand, within BWI76_RS15440 at 3236.776 kb on - strand at 3236.863 kb on + strand at 3236.869 kb on + strand at 3236.892 kb on + strand at 3236.899 kb on + strand at 3236.904 kb on + strand at 3236.907 kb on - strand at 3236.924 kb on - strand at 3236.953 kb on + strand at 3236.961 kb on - strand at 3236.978 kb on - strand at 3237.015 kb on + strand at 3237.015 kb on + strand at 3237.015 kb on + strand at 3237.015 kb on + strand at 3237.165 kb on - strand, within BWI76_RS15445 at 3237.170 kb on + strand, within BWI76_RS15445 at 3237.198 kb on - strand, within BWI76_RS15445 at 3237.212 kb on - strand, within BWI76_RS15445 at 3237.228 kb on + strand, within BWI76_RS15445 at 3237.250 kb on + strand, within BWI76_RS15445 at 3237.356 kb on - strand at 3237.360 kb on + strand at 3237.368 kb on - strand at 3237.368 kb on - strand at 3237.389 kb on + strand at 3237.448 kb on + strand, within BWI76_RS15450 at 3237.456 kb on - strand, within BWI76_RS15450 at 3237.456 kb on - strand, within BWI76_RS15450 at 3237.610 kb on - strand, within BWI76_RS15450 at 3237.781 kb on + strand at 3237.781 kb on + strand at 3237.801 kb on + strand at 3237.809 kb on - strand at 3237.855 kb on + strand at 3237.863 kb on - strand at 3237.911 kb on + strand, within BWI76_RS15455 at 3237.919 kb on - strand, within BWI76_RS15455 at 3237.919 kb on - strand, within BWI76_RS15455 at 3237.919 kb on - strand, within BWI76_RS15455 at 3237.919 kb on - strand, within BWI76_RS15455 at 3237.946 kb on - strand, within BWI76_RS15455 at 3237.947 kb on + strand, within BWI76_RS15455 at 3238.001 kb on + strand, within BWI76_RS15455 at 3238.045 kb on + strand, within BWI76_RS15455 at 3238.053 kb on - strand, within BWI76_RS15455 at 3238.104 kb on + strand, within BWI76_RS15455 at 3238.154 kb on + strand, within BWI76_RS15455 at 3238.172 kb on + strand, within BWI76_RS15455 at 3238.172 kb on + strand, within BWI76_RS15455 at 3238.185 kb on - strand, within BWI76_RS15455 at 3238.295 kb on + strand at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.303 kb on - strand, within BWI76_RS15455 at 3238.303 kb on - strand, within BWI76_RS15455 at 3238.314 kb on + strand, within BWI76_RS15455 at 3238.327 kb on + strand, within BWI76_RS15455 at 3238.383 kb on - strand at 3238.385 kb on + strand at 3238.393 kb on - strand at 3238.395 kb on + strand at 3238.398 kb on - strand at 3238.403 kb on - strand at 3238.421 kb on - strand at 3238.421 kb on - strand at 3238.428 kb on + strand at 3238.436 kb on + strand at 3238.444 kb on - strand at 3238.444 kb on - strand at 3238.481 kb on - strand at 3238.501 kb on - strand at 3238.507 kb on + strand at 3238.534 kb on - strand at 3238.596 kb on + strand, within BWI76_RS15460 at 3238.596 kb on + strand, within BWI76_RS15460 at 3238.596 kb on + strand, within BWI76_RS15460 at 3238.596 kb on + strand, within BWI76_RS15460 at 3238.596 kb on + strand, within BWI76_RS15460 at 3238.596 kb on + strand, within BWI76_RS15460 at 3238.604 kb on - strand, within BWI76_RS15460 at 3238.604 kb on - strand, within BWI76_RS15460 at 3238.606 kb on + strand, within BWI76_RS15460
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 3,236,410 + +0.1 3,236,567 - BWI76_RS15440 0.67 +0.2 3,236,581 + BWI76_RS15440 0.72 +0.2 3,236,776 - +0.2 3,236,863 + -2.7 3,236,869 + -0.2 3,236,892 + -0.6 3,236,899 + +0.1 3,236,904 + +1.7 3,236,907 - +0.4 3,236,924 - -0.0 3,236,953 + +0.0 3,236,961 - +0.1 3,236,978 - +0.9 3,237,015 + +0.2 3,237,015 + +0.1 3,237,015 + -0.5 3,237,015 + +0.0 3,237,165 - BWI76_RS15445 0.49 -2.8 3,237,170 + BWI76_RS15445 0.51 -0.6 3,237,198 - BWI76_RS15445 0.61 -0.4 3,237,212 - BWI76_RS15445 0.66 -0.8 3,237,228 + BWI76_RS15445 0.72 +2.9 3,237,250 + BWI76_RS15445 0.80 -3.2 3,237,356 - +0.1 3,237,360 + +0.4 3,237,368 - +0.4 3,237,368 - -0.1 3,237,389 + +0.2 3,237,448 + BWI76_RS15450 0.26 -2.1 3,237,456 - BWI76_RS15450 0.29 +0.0 3,237,456 - BWI76_RS15450 0.29 -2.1 3,237,610 - BWI76_RS15450 0.78 -1.6 3,237,781 + -1.1 3,237,781 + -1.1 3,237,801 + -0.0 3,237,809 - +0.3 3,237,855 + -0.6 3,237,863 - -0.2 3,237,911 + BWI76_RS15455 0.13 +0.2 3,237,919 - BWI76_RS15455 0.14 -1.0 3,237,919 - BWI76_RS15455 0.14 -0.8 3,237,919 - BWI76_RS15455 0.14 +0.8 3,237,919 - BWI76_RS15455 0.14 +0.0 3,237,946 - BWI76_RS15455 0.19 +0.5 3,237,947 + BWI76_RS15455 0.19 +1.2 3,238,001 + BWI76_RS15455 0.29 -1.0 3,238,045 + BWI76_RS15455 0.38 -1.5 3,238,053 - BWI76_RS15455 0.39 -1.6 3,238,104 + BWI76_RS15455 0.49 -1.5 3,238,154 + BWI76_RS15455 0.58 -0.7 3,238,172 + BWI76_RS15455 0.61 -1.1 3,238,172 + BWI76_RS15455 0.61 -0.1 3,238,185 - BWI76_RS15455 0.64 +1.2 3,238,295 + -1.4 3,238,295 + BWI76_RS15455 0.84 -2.8 3,238,295 + BWI76_RS15455 0.84 +0.6 3,238,295 + BWI76_RS15455 0.84 -0.1 3,238,295 + BWI76_RS15455 0.84 +1.1 3,238,295 + BWI76_RS15455 0.84 +0.4 3,238,295 + BWI76_RS15455 0.84 -1.4 3,238,295 + -1.1 3,238,295 + BWI76_RS15455 0.84 -1.3 3,238,295 + BWI76_RS15455 0.84 +0.3 3,238,303 - BWI76_RS15455 0.85 +0.5 3,238,303 - BWI76_RS15455 0.85 -2.4 3,238,314 + BWI76_RS15455 0.87 -1.6 3,238,327 + BWI76_RS15455 0.90 -1.1 3,238,383 - -1.1 3,238,385 + +0.3 3,238,393 - +1.8 3,238,395 + -3.2 3,238,398 - -0.8 3,238,403 - +0.2 3,238,421 - +1.5 3,238,421 - -0.1 3,238,428 + -0.8 3,238,436 + +0.3 3,238,444 - -2.5 3,238,444 - -1.1 3,238,481 - -2.5 3,238,501 - -1.0 3,238,507 + -3.9 3,238,534 - +0.7 3,238,596 + BWI76_RS15460 0.21 -2.7 3,238,596 + BWI76_RS15460 0.21 -0.2 3,238,596 + BWI76_RS15460 0.21 -1.6 3,238,596 + BWI76_RS15460 0.21 -1.4 3,238,596 + BWI76_RS15460 0.21 -1.4 3,238,596 + BWI76_RS15460 0.21 +0.7 3,238,604 - BWI76_RS15460 0.23 +1.3 3,238,604 - BWI76_RS15460 0.23 -0.7 3,238,606 + BWI76_RS15460 0.23 +0.0
Or see this region's nucleotide sequence