Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14750 and BWI76_RS14755 are separated by 14 nucleotides BWI76_RS14755 and BWI76_RS14760 are separated by 16 nucleotides BWI76_RS14760 and BWI76_RS14765 overlap by 4 nucleotides BWI76_RS14765 and BWI76_RS14770 are separated by 25 nucleotides BWI76_RS14770 and BWI76_RS14775 overlap by 8 nucleotides
BWI76_RS14750: BWI76_RS14750 - transcriptional regulator, at 3,095,184 to 3,095,939
_RS14750
BWI76_RS14755: BWI76_RS14755 - SIS domain-containing protein, at 3,095,954 to 3,096,694
_RS14755
BWI76_RS14760: BWI76_RS14760 - PTS ascorbate transporter subunit IIC, at 3,096,711 to 3,098,021
_RS14760
BWI76_RS14765: BWI76_RS14765 - PTS lactose/cellobiose transporter subunit IIB, at 3,098,018 to 3,098,305
_RS14765
BWI76_RS14770: BWI76_RS14770 - PTS IIA 2 domain protein, at 3,098,331 to 3,098,774
_RS14770
BWI76_RS14775: BWI76_RS14775 - creatinine amidohydrolase, at 3,098,767 to 3,099,507
_RS14775
Position (kb)
3096
3097
3098
3099 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3095.740 kb on + strand, within BWI76_RS14750 at 3095.748 kb on - strand, within BWI76_RS14750 at 3095.750 kb on + strand, within BWI76_RS14750 at 3095.773 kb on - strand, within BWI76_RS14750 at 3095.778 kb on - strand, within BWI76_RS14750 at 3095.905 kb on - strand at 3096.093 kb on - strand, within BWI76_RS14755 at 3096.210 kb on + strand, within BWI76_RS14755 at 3096.482 kb on - strand, within BWI76_RS14755 at 3096.482 kb on - strand, within BWI76_RS14755 at 3096.647 kb on - strand at 3096.859 kb on - strand, within BWI76_RS14760 at 3097.093 kb on - strand, within BWI76_RS14760 at 3097.141 kb on - strand, within BWI76_RS14760 at 3097.387 kb on - strand, within BWI76_RS14760 at 3097.568 kb on - strand, within BWI76_RS14760 at 3097.595 kb on + strand, within BWI76_RS14760 at 3097.723 kb on - strand, within BWI76_RS14760 at 3097.780 kb on - strand, within BWI76_RS14760 at 3097.816 kb on + strand, within BWI76_RS14760 at 3097.877 kb on - strand, within BWI76_RS14760 at 3097.943 kb on - strand at 3098.043 kb on + strand at 3098.104 kb on + strand, within BWI76_RS14765 at 3098.169 kb on - strand, within BWI76_RS14765 at 3098.218 kb on - strand, within BWI76_RS14765 at 3098.449 kb on - strand, within BWI76_RS14770 at 3098.731 kb on - strand at 3098.906 kb on + strand, within BWI76_RS14775
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 3,095,740 + BWI76_RS14750 0.74 +0.3 3,095,748 - BWI76_RS14750 0.75 +0.6 3,095,750 + BWI76_RS14750 0.75 +0.4 3,095,773 - BWI76_RS14750 0.78 -0.8 3,095,778 - BWI76_RS14750 0.79 -0.3 3,095,905 - +0.1 3,096,093 - BWI76_RS14755 0.19 -1.9 3,096,210 + BWI76_RS14755 0.35 -0.5 3,096,482 - BWI76_RS14755 0.71 -0.9 3,096,482 - BWI76_RS14755 0.71 -1.6 3,096,647 - +0.6 3,096,859 - BWI76_RS14760 0.11 +1.4 3,097,093 - BWI76_RS14760 0.29 +0.6 3,097,141 - BWI76_RS14760 0.33 +0.8 3,097,387 - BWI76_RS14760 0.52 +0.1 3,097,568 - BWI76_RS14760 0.65 -1.6 3,097,595 + BWI76_RS14760 0.67 +1.1 3,097,723 - BWI76_RS14760 0.77 +1.4 3,097,780 - BWI76_RS14760 0.82 +0.6 3,097,816 + BWI76_RS14760 0.84 -1.0 3,097,877 - BWI76_RS14760 0.89 -2.9 3,097,943 - -0.8 3,098,043 + +1.3 3,098,104 + BWI76_RS14765 0.30 -0.2 3,098,169 - BWI76_RS14765 0.52 +0.5 3,098,218 - BWI76_RS14765 0.69 +0.3 3,098,449 - BWI76_RS14770 0.27 -1.5 3,098,731 - +0.1 3,098,906 + BWI76_RS14775 0.19 +0.8
Or see this region's nucleotide sequence