Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14650 and BWI76_RS14655 are separated by 140 nucleotides
BWI76_RS14650: BWI76_RS14650 - helix-turn-helix transcriptional regulator, at 3,076,631 to 3,077,605
_RS14650
BWI76_RS14655: BWI76_RS14655 - FKBP-type peptidyl-prolyl isomerase domain-containing protein, at 3,077,746 to 3,079,032
_RS14655
Position (kb)
3076
3077
3078 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3075.833 kb on + strand at 3075.843 kb on + strand at 3075.909 kb on + strand at 3075.943 kb on + strand at 3076.097 kb on - strand at 3076.161 kb on + strand at 3076.185 kb on + strand at 3076.193 kb on - strand at 3076.217 kb on + strand at 3076.374 kb on - strand at 3076.390 kb on + strand at 3076.391 kb on + strand at 3076.408 kb on - strand at 3076.418 kb on - strand at 3076.458 kb on - strand at 3076.458 kb on - strand at 3076.458 kb on - strand at 3076.458 kb on - strand at 3076.463 kb on - strand at 3076.479 kb on - strand at 3076.498 kb on + strand at 3076.501 kb on + strand at 3076.506 kb on - strand at 3076.506 kb on - strand at 3076.506 kb on - strand at 3076.506 kb on - strand at 3076.506 kb on - strand at 3076.507 kb on - strand at 3076.509 kb on - strand at 3076.509 kb on - strand at 3076.514 kb on - strand at 3076.591 kb on - strand at 3076.609 kb on + strand at 3076.620 kb on - strand at 3076.630 kb on - strand at 3076.635 kb on - strand at 3076.662 kb on - strand at 3076.662 kb on - strand at 3076.686 kb on - strand at 3076.703 kb on - strand at 3076.980 kb on + strand at 3076.985 kb on + strand, within BWI76_RS14650 at 3077.162 kb on - strand, within BWI76_RS14650 at 3077.162 kb on - strand, within BWI76_RS14650 at 3077.162 kb on - strand, within BWI76_RS14650 at 3077.163 kb on - strand at 3077.164 kb on + strand, within BWI76_RS14650 at 3077.326 kb on + strand, within BWI76_RS14650 at 3077.738 kb on - strand at 3077.802 kb on + strand at 3077.860 kb on - strand at 3077.898 kb on - strand, within BWI76_RS14655 at 3078.013 kb on + strand, within BWI76_RS14655 at 3078.021 kb on - strand, within BWI76_RS14655 at 3078.041 kb on + strand at 3078.046 kb on + strand, within BWI76_RS14655 at 3078.046 kb on + strand, within BWI76_RS14655 at 3078.046 kb on + strand, within BWI76_RS14655 at 3078.046 kb on + strand, within BWI76_RS14655 at 3078.049 kb on - strand, within BWI76_RS14655 at 3078.054 kb on - strand, within BWI76_RS14655 at 3078.054 kb on - strand, within BWI76_RS14655 at 3078.160 kb on + strand, within BWI76_RS14655 at 3078.168 kb on - strand, within BWI76_RS14655 at 3078.170 kb on + strand, within BWI76_RS14655 at 3078.183 kb on - strand, within BWI76_RS14655 at 3078.184 kb on - strand, within BWI76_RS14655 at 3078.186 kb on + strand, within BWI76_RS14655 at 3078.193 kb on + strand, within BWI76_RS14655 at 3078.195 kb on + strand, within BWI76_RS14655 at 3078.195 kb on + strand, within BWI76_RS14655 at 3078.195 kb on + strand, within BWI76_RS14655 at 3078.197 kb on + strand, within BWI76_RS14655 at 3078.203 kb on - strand, within BWI76_RS14655 at 3078.213 kb on - strand, within BWI76_RS14655 at 3078.226 kb on - strand, within BWI76_RS14655 at 3078.262 kb on + strand, within BWI76_RS14655 at 3078.270 kb on - strand, within BWI76_RS14655 at 3078.305 kb on - strand, within BWI76_RS14655 at 3078.305 kb on - strand, within BWI76_RS14655 at 3078.305 kb on - strand, within BWI76_RS14655 at 3078.305 kb on - strand, within BWI76_RS14655 at 3078.305 kb on - strand at 3078.305 kb on - strand at 3078.356 kb on + strand, within BWI76_RS14655 at 3078.364 kb on - strand, within BWI76_RS14655 at 3078.364 kb on - strand, within BWI76_RS14655 at 3078.376 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 3,075,833 + -0.2 3,075,843 + -0.9 3,075,909 + -0.5 3,075,943 + -3.4 3,076,097 - -1.0 3,076,161 + +0.7 3,076,185 + -1.0 3,076,193 - +0.2 3,076,217 + -2.3 3,076,374 - -0.4 3,076,390 + -1.4 3,076,391 + +1.1 3,076,408 - +0.4 3,076,418 - +0.4 3,076,458 - -2.2 3,076,458 - +0.3 3,076,458 - -2.3 3,076,458 - -2.6 3,076,463 - +0.4 3,076,479 - -1.2 3,076,498 + +0.5 3,076,501 + +1.1 3,076,506 - -0.3 3,076,506 - -0.9 3,076,506 - +0.9 3,076,506 - -1.4 3,076,506 - -1.0 3,076,507 - +1.1 3,076,509 - -3.1 3,076,509 - +0.6 3,076,514 - +0.4 3,076,591 - -0.4 3,076,609 + -1.2 3,076,620 - -0.1 3,076,630 - +1.1 3,076,635 - -0.4 3,076,662 - +1.1 3,076,662 - -0.4 3,076,686 - +0.5 3,076,703 - +0.1 3,076,980 + +1.5 3,076,985 + BWI76_RS14650 0.36 -0.9 3,077,162 - BWI76_RS14650 0.54 -0.9 3,077,162 - BWI76_RS14650 0.54 -1.6 3,077,162 - BWI76_RS14650 0.54 +1.1 3,077,163 - -0.4 3,077,164 + BWI76_RS14650 0.55 +0.1 3,077,326 + BWI76_RS14650 0.71 +0.1 3,077,738 - -0.4 3,077,802 + +0.1 3,077,860 - -2.4 3,077,898 - BWI76_RS14655 0.12 -1.7 3,078,013 + BWI76_RS14655 0.21 +1.7 3,078,021 - BWI76_RS14655 0.21 +0.3 3,078,041 + +1.1 3,078,046 + BWI76_RS14655 0.23 +1.1 3,078,046 + BWI76_RS14655 0.23 -2.0 3,078,046 + BWI76_RS14655 0.23 -0.9 3,078,046 + BWI76_RS14655 0.23 -1.0 3,078,049 - BWI76_RS14655 0.24 +0.2 3,078,054 - BWI76_RS14655 0.24 -1.9 3,078,054 - BWI76_RS14655 0.24 +0.1 3,078,160 + BWI76_RS14655 0.32 -0.7 3,078,168 - BWI76_RS14655 0.33 +0.8 3,078,170 + BWI76_RS14655 0.33 +0.6 3,078,183 - BWI76_RS14655 0.34 +0.7 3,078,184 - BWI76_RS14655 0.34 +1.1 3,078,186 + BWI76_RS14655 0.34 +1.1 3,078,193 + BWI76_RS14655 0.35 +1.1 3,078,195 + BWI76_RS14655 0.35 -0.4 3,078,195 + BWI76_RS14655 0.35 +0.9 3,078,195 + BWI76_RS14655 0.35 -0.4 3,078,197 + BWI76_RS14655 0.35 +2.5 3,078,203 - BWI76_RS14655 0.36 +0.1 3,078,213 - BWI76_RS14655 0.36 -1.7 3,078,226 - BWI76_RS14655 0.37 -0.3 3,078,262 + BWI76_RS14655 0.40 +0.6 3,078,270 - BWI76_RS14655 0.41 -1.4 3,078,305 - BWI76_RS14655 0.43 -0.2 3,078,305 - BWI76_RS14655 0.43 -0.4 3,078,305 - BWI76_RS14655 0.43 -2.3 3,078,305 - BWI76_RS14655 0.43 +1.1 3,078,305 - +1.1 3,078,305 - +1.1 3,078,356 + BWI76_RS14655 0.47 +0.4 3,078,364 - BWI76_RS14655 0.48 -1.9 3,078,364 - BWI76_RS14655 0.48 +0.1 3,078,376 - +1.1
Or see this region's nucleotide sequence