Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14490 and BWI76_RS14495 are separated by 134 nucleotides BWI76_RS14495 and BWI76_RS14500 are separated by 32 nucleotides BWI76_RS14500 and BWI76_RS14505 are separated by 69 nucleotides
BWI76_RS14490: BWI76_RS14490 - ABC transporter substrate-binding protein, at 3,025,420 to 3,026,691
_RS14490
BWI76_RS14495: BWI76_RS14495 - hypothetical protein, at 3,026,826 to 3,027,014
_RS14495
BWI76_RS14500: BWI76_RS14500 - MFS transporter, at 3,027,047 to 3,028,438
_RS14500
BWI76_RS14505: BWI76_RS14505 - salicylate synthase Irp9, at 3,028,508 to 3,029,818
_RS14505
Position (kb) 3027
3028
3029 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3026.051 kb on - strand, within BWI76_RS14490 at 3026.114 kb on - strand, within BWI76_RS14490 at 3026.237 kb on + strand, within BWI76_RS14490 at 3026.588 kb on - strand at 3026.672 kb on - strand at 3026.711 kb on + strand at 3026.722 kb on + strand at 3026.731 kb on - strand at 3027.016 kb on + strand at 3027.110 kb on + strand at 3027.126 kb on + strand at 3027.157 kb on - strand at 3027.159 kb on + strand at 3027.168 kb on + strand at 3027.173 kb on + strand at 3027.176 kb on - strand at 3027.176 kb on - strand at 3027.176 kb on - strand at 3027.186 kb on + strand at 3027.193 kb on - strand, within BWI76_RS14500 at 3027.196 kb on + strand, within BWI76_RS14500 at 3027.196 kb on + strand, within BWI76_RS14500 at 3027.196 kb on + strand, within BWI76_RS14500 at 3027.204 kb on - strand, within BWI76_RS14500 at 3027.204 kb on - strand, within BWI76_RS14500 at 3027.204 kb on - strand, within BWI76_RS14500 at 3027.204 kb on - strand, within BWI76_RS14500 at 3027.204 kb on - strand, within BWI76_RS14500 at 3027.204 kb on - strand, within BWI76_RS14500 at 3027.218 kb on + strand, within BWI76_RS14500 at 3027.258 kb on + strand, within BWI76_RS14500 at 3027.258 kb on + strand, within BWI76_RS14500 at 3027.266 kb on - strand, within BWI76_RS14500 at 3027.301 kb on - strand, within BWI76_RS14500 at 3027.319 kb on - strand, within BWI76_RS14500 at 3027.353 kb on - strand, within BWI76_RS14500 at 3027.429 kb on - strand, within BWI76_RS14500 at 3027.441 kb on - strand, within BWI76_RS14500 at 3027.675 kb on - strand, within BWI76_RS14500 at 3027.695 kb on - strand, within BWI76_RS14500 at 3027.707 kb on + strand, within BWI76_RS14500 at 3027.798 kb on - strand, within BWI76_RS14500 at 3027.852 kb on + strand, within BWI76_RS14500 at 3027.874 kb on + strand, within BWI76_RS14500 at 3028.049 kb on - strand, within BWI76_RS14500 at 3028.203 kb on - strand, within BWI76_RS14500 at 3028.251 kb on + strand, within BWI76_RS14500 at 3028.342 kb on + strand at 3028.426 kb on - strand at 3028.527 kb on - strand at 3028.527 kb on - strand at 3028.565 kb on - strand at 3028.983 kb on - strand, within BWI76_RS14505 at 3029.349 kb on - strand, within BWI76_RS14505
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 3,026,051 - BWI76_RS14490 0.50 -1.2 3,026,114 - BWI76_RS14490 0.55 +0.2 3,026,237 + BWI76_RS14490 0.64 -1.2 3,026,588 - -1.7 3,026,672 - +0.4 3,026,711 + +0.6 3,026,722 + +0.2 3,026,731 - +0.3 3,027,016 + +1.2 3,027,110 + -0.8 3,027,126 + -2.5 3,027,157 - -0.7 3,027,159 + -1.0 3,027,168 + -1.2 3,027,173 + -0.2 3,027,176 - -2.0 3,027,176 - -2.7 3,027,176 - -0.2 3,027,186 + -1.0 3,027,193 - BWI76_RS14500 0.10 +0.8 3,027,196 + BWI76_RS14500 0.11 +0.6 3,027,196 + BWI76_RS14500 0.11 +0.5 3,027,196 + BWI76_RS14500 0.11 +0.0 3,027,204 - BWI76_RS14500 0.11 +0.1 3,027,204 - BWI76_RS14500 0.11 +0.2 3,027,204 - BWI76_RS14500 0.11 +1.8 3,027,204 - BWI76_RS14500 0.11 -2.2 3,027,204 - BWI76_RS14500 0.11 -2.2 3,027,204 - BWI76_RS14500 0.11 -0.4 3,027,218 + BWI76_RS14500 0.12 +0.5 3,027,258 + BWI76_RS14500 0.15 +1.6 3,027,258 + BWI76_RS14500 0.15 +0.3 3,027,266 - BWI76_RS14500 0.16 -2.9 3,027,301 - BWI76_RS14500 0.18 -1.7 3,027,319 - BWI76_RS14500 0.20 -3.4 3,027,353 - BWI76_RS14500 0.22 -2.5 3,027,429 - BWI76_RS14500 0.27 +0.1 3,027,441 - BWI76_RS14500 0.28 -0.5 3,027,675 - BWI76_RS14500 0.45 -0.3 3,027,695 - BWI76_RS14500 0.47 +0.4 3,027,707 + BWI76_RS14500 0.47 +1.2 3,027,798 - BWI76_RS14500 0.54 +0.4 3,027,852 + BWI76_RS14500 0.58 +2.7 3,027,874 + BWI76_RS14500 0.59 +1.7 3,028,049 - BWI76_RS14500 0.72 +1.3 3,028,203 - BWI76_RS14500 0.83 -0.1 3,028,251 + BWI76_RS14500 0.86 +1.3 3,028,342 + -0.6 3,028,426 - +0.7 3,028,527 - -0.4 3,028,527 - +0.3 3,028,565 - +1.2 3,028,983 - BWI76_RS14505 0.36 +1.3 3,029,349 - BWI76_RS14505 0.64 -0.1
Or see this region's nucleotide sequence