Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14300 and BWI76_RS14305 are separated by 87 nucleotides BWI76_RS14305 and BWI76_RS14310 are separated by 4 nucleotides BWI76_RS14310 and BWI76_RS14315 overlap by 1 nucleotides BWI76_RS14315 and BWI76_RS14320 are separated by 1 nucleotides
BWI76_RS14295: BWI76_RS14295 - IS630 family transposase, at 2,988,739 to 2,989,680
_RS14295
BWI76_RS14300: BWI76_RS14300 - hypothetical protein, at 2,989,609 to 2,989,795
_RS14300
BWI76_RS14305: BWI76_RS14305 - metallophosphoesterase, at 2,989,883 to 2,990,668
_RS14305
BWI76_RS14310: BWI76_RS14310 - hypothetical protein, at 2,990,673 to 2,991,554
_RS14310
BWI76_RS14315: BWI76_RS14315 - MazG nucleotide pyrophosphohydrolase, at 2,991,554 to 2,991,892
_RS14315
BWI76_RS14320: BWI76_RS14320 - hypothetical protein, at 2,991,894 to 2,992,829
_RS14320
Position (kb)
2990
2991
2992 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2989.701 kb on + strand, within BWI76_RS14300 at 2989.715 kb on - strand, within BWI76_RS14300 at 2989.728 kb on + strand, within BWI76_RS14300 at 2989.731 kb on - strand, within BWI76_RS14300 at 2989.731 kb on - strand at 2989.801 kb on + strand at 2989.806 kb on + strand at 2990.042 kb on + strand, within BWI76_RS14305 at 2990.047 kb on + strand, within BWI76_RS14305 at 2990.321 kb on + strand, within BWI76_RS14305 at 2990.390 kb on + strand, within BWI76_RS14305 at 2990.405 kb on + strand, within BWI76_RS14305 at 2990.471 kb on + strand, within BWI76_RS14305 at 2990.471 kb on + strand, within BWI76_RS14305 at 2990.513 kb on + strand, within BWI76_RS14305 at 2990.513 kb on + strand, within BWI76_RS14305 at 2990.838 kb on + strand, within BWI76_RS14310 at 2990.838 kb on + strand, within BWI76_RS14310 at 2990.840 kb on + strand, within BWI76_RS14310 at 2990.840 kb on + strand at 2990.848 kb on + strand, within BWI76_RS14310 at 2990.850 kb on + strand, within BWI76_RS14310 at 2990.985 kb on + strand, within BWI76_RS14310 at 2991.214 kb on + strand, within BWI76_RS14310 at 2991.275 kb on + strand, within BWI76_RS14310 at 2991.275 kb on + strand, within BWI76_RS14310 at 2991.275 kb on + strand, within BWI76_RS14310 at 2991.294 kb on + strand, within BWI76_RS14310 at 2991.307 kb on + strand at 2991.320 kb on + strand, within BWI76_RS14310 at 2991.326 kb on + strand, within BWI76_RS14310 at 2991.327 kb on + strand, within BWI76_RS14310 at 2991.344 kb on + strand, within BWI76_RS14310 at 2991.350 kb on + strand, within BWI76_RS14310 at 2991.374 kb on + strand, within BWI76_RS14310 at 2991.374 kb on + strand, within BWI76_RS14310 at 2991.452 kb on + strand, within BWI76_RS14310 at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.491 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2991.498 kb on + strand at 2992.011 kb on + strand, within BWI76_RS14320 at 2992.114 kb on + strand, within BWI76_RS14320 at 2992.201 kb on + strand, within BWI76_RS14320 at 2992.293 kb on + strand at 2992.293 kb on + strand, within BWI76_RS14320 at 2992.395 kb on + strand, within BWI76_RS14320
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 2,989,701 + BWI76_RS14300 0.49 +0.5 2,989,715 - BWI76_RS14300 0.57 -1.7 2,989,728 + BWI76_RS14300 0.64 -2.4 2,989,731 - BWI76_RS14300 0.65 +0.5 2,989,731 - -1.2 2,989,801 + +0.1 2,989,806 + -1.2 2,990,042 + BWI76_RS14305 0.20 -2.8 2,990,047 + BWI76_RS14305 0.21 +1.3 2,990,321 + BWI76_RS14305 0.56 +2.3 2,990,390 + BWI76_RS14305 0.65 -1.7 2,990,405 + BWI76_RS14305 0.66 -0.9 2,990,471 + BWI76_RS14305 0.75 -0.1 2,990,471 + BWI76_RS14305 0.75 -2.3 2,990,513 + BWI76_RS14305 0.80 -2.6 2,990,513 + BWI76_RS14305 0.80 -2.7 2,990,838 + BWI76_RS14310 0.19 +0.1 2,990,838 + BWI76_RS14310 0.19 -2.4 2,990,840 + BWI76_RS14310 0.19 +1.7 2,990,840 + +1.1 2,990,848 + BWI76_RS14310 0.20 -0.9 2,990,850 + BWI76_RS14310 0.20 -3.6 2,990,985 + BWI76_RS14310 0.35 -3.3 2,991,214 + BWI76_RS14310 0.61 -2.0 2,991,275 + BWI76_RS14310 0.68 -0.9 2,991,275 + BWI76_RS14310 0.68 -1.3 2,991,275 + BWI76_RS14310 0.68 +1.1 2,991,294 + BWI76_RS14310 0.70 +1.1 2,991,307 + +2.7 2,991,320 + BWI76_RS14310 0.73 +0.1 2,991,326 + BWI76_RS14310 0.74 -1.2 2,991,327 + BWI76_RS14310 0.74 -0.1 2,991,344 + BWI76_RS14310 0.76 -0.9 2,991,350 + BWI76_RS14310 0.77 -1.4 2,991,374 + BWI76_RS14310 0.79 -0.9 2,991,374 + BWI76_RS14310 0.79 -1.2 2,991,452 + BWI76_RS14310 0.88 -1.6 2,991,491 + -3.4 2,991,491 + +1.2 2,991,491 + -2.4 2,991,491 + -0.4 2,991,491 + -0.2 2,991,491 + -1.4 2,991,491 + -0.4 2,991,498 + -0.9 2,991,498 + -1.4 2,991,498 + -2.0 2,991,498 + -1.3 2,991,498 + -2.2 2,991,498 + +2.6 2,992,011 + BWI76_RS14320 0.12 -1.2 2,992,114 + BWI76_RS14320 0.24 -1.9 2,992,201 + BWI76_RS14320 0.33 +1.1 2,992,293 + -1.0 2,992,293 + BWI76_RS14320 0.43 +0.1 2,992,395 + BWI76_RS14320 0.54 -1.9
Or see this region's nucleotide sequence