Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13850 and BWI76_RS13855 are separated by 79 nucleotides BWI76_RS13855 and BWI76_RS13860 are separated by 115 nucleotides BWI76_RS13860 and BWI76_RS13865 are separated by 197 nucleotides
BWI76_RS13850: BWI76_RS13850 - N-acetyltransferase, at 2,889,237 to 2,889,716
_RS13850
BWI76_RS13855: BWI76_RS13855 - peroxiredoxin, at 2,889,796 to 2,890,227
_RS13855
BWI76_RS13860: BWI76_RS13860 - hypothetical protein, at 2,890,343 to 2,891,212
_RS13860
BWI76_RS13865: BWI76_RS13865 - cation diffusion facilitator family transporter, at 2,891,410 to 2,892,318
_RS13865
Position (kb)
2890
2891
2892 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2889.458 kb on + strand, within BWI76_RS13850 at 2889.509 kb on - strand, within BWI76_RS13850 at 2889.546 kb on + strand, within BWI76_RS13850 at 2889.654 kb on - strand, within BWI76_RS13850 at 2889.718 kb on - strand at 2889.763 kb on - strand at 2889.919 kb on + strand, within BWI76_RS13855 at 2889.989 kb on + strand, within BWI76_RS13855 at 2890.008 kb on + strand, within BWI76_RS13855 at 2890.215 kb on + strand at 2890.256 kb on + strand at 2890.423 kb on + strand at 2890.608 kb on - strand, within BWI76_RS13860 at 2890.779 kb on + strand, within BWI76_RS13860 at 2890.787 kb on - strand, within BWI76_RS13860 at 2891.019 kb on - strand, within BWI76_RS13860 at 2891.077 kb on - strand, within BWI76_RS13860 at 2891.097 kb on + strand, within BWI76_RS13860 at 2891.097 kb on + strand, within BWI76_RS13860 at 2891.097 kb on + strand, within BWI76_RS13860 at 2891.105 kb on - strand, within BWI76_RS13860 at 2891.105 kb on - strand, within BWI76_RS13860 at 2891.107 kb on + strand, within BWI76_RS13860 at 2891.131 kb on + strand at 2891.137 kb on - strand at 2891.139 kb on - strand at 2891.142 kb on + strand at 2891.154 kb on + strand at 2891.154 kb on + strand at 2891.160 kb on - strand at 2891.160 kb on - strand at 2891.162 kb on - strand at 2891.164 kb on + strand at 2891.164 kb on + strand at 2891.167 kb on + strand at 2891.167 kb on + strand at 2891.174 kb on + strand at 2891.174 kb on + strand at 2891.174 kb on + strand at 2891.174 kb on + strand at 2891.174 kb on + strand at 2891.175 kb on - strand at 2891.179 kb on + strand at 2891.180 kb on - strand at 2891.182 kb on - strand at 2891.184 kb on + strand at 2891.186 kb on + strand at 2891.187 kb on - strand at 2891.195 kb on - strand at 2891.195 kb on - strand at 2891.240 kb on - strand at 2891.381 kb on - strand at 2891.430 kb on + strand at 2891.438 kb on + strand at 2891.446 kb on - strand at 2891.456 kb on + strand at 2891.461 kb on + strand at 2891.466 kb on + strand at 2891.500 kb on + strand at 2891.506 kb on - strand at 2891.511 kb on + strand, within BWI76_RS13865 at 2891.627 kb on - strand, within BWI76_RS13865 at 2891.628 kb on - strand, within BWI76_RS13865 at 2891.637 kb on + strand, within BWI76_RS13865 at 2891.645 kb on - strand, within BWI76_RS13865 at 2891.653 kb on - strand, within BWI76_RS13865 at 2891.701 kb on - strand, within BWI76_RS13865 at 2891.723 kb on - strand, within BWI76_RS13865 at 2891.783 kb on + strand, within BWI76_RS13865 at 2891.858 kb on + strand, within BWI76_RS13865 at 2892.000 kb on - strand, within BWI76_RS13865 at 2892.004 kb on - strand, within BWI76_RS13865 at 2892.015 kb on - strand, within BWI76_RS13865 at 2892.015 kb on - strand, within BWI76_RS13865 at 2892.058 kb on - strand, within BWI76_RS13865 at 2892.115 kb on - strand, within BWI76_RS13865 at 2892.115 kb on - strand, within BWI76_RS13865 at 2892.171 kb on + strand, within BWI76_RS13865
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 2,889,458 + BWI76_RS13850 0.46 +0.3 2,889,509 - BWI76_RS13850 0.57 -0.6 2,889,546 + BWI76_RS13850 0.64 -0.2 2,889,654 - BWI76_RS13850 0.87 +1.1 2,889,718 - -1.1 2,889,763 - +1.5 2,889,919 + BWI76_RS13855 0.28 -1.8 2,889,989 + BWI76_RS13855 0.45 +0.5 2,890,008 + BWI76_RS13855 0.49 +0.0 2,890,215 + +0.6 2,890,256 + +0.5 2,890,423 + -2.1 2,890,608 - BWI76_RS13860 0.30 -0.4 2,890,779 + BWI76_RS13860 0.50 -2.2 2,890,787 - BWI76_RS13860 0.51 -2.1 2,891,019 - BWI76_RS13860 0.78 -0.6 2,891,077 - BWI76_RS13860 0.84 -0.5 2,891,097 + BWI76_RS13860 0.87 -1.0 2,891,097 + BWI76_RS13860 0.87 -0.0 2,891,097 + BWI76_RS13860 0.87 +0.3 2,891,105 - BWI76_RS13860 0.88 -2.0 2,891,105 - BWI76_RS13860 0.88 -0.4 2,891,107 + BWI76_RS13860 0.88 -0.1 2,891,131 + +1.1 2,891,137 - +0.1 2,891,139 - +0.8 2,891,142 + -2.2 2,891,154 + +1.1 2,891,154 + -2.4 2,891,160 - -0.9 2,891,160 - +1.0 2,891,162 - -0.5 2,891,164 + -0.1 2,891,164 + -1.0 2,891,167 + +0.9 2,891,167 + +0.1 2,891,174 + -1.0 2,891,174 + -1.1 2,891,174 + +0.5 2,891,174 + -3.1 2,891,174 + +0.1 2,891,175 - -2.7 2,891,179 + +0.1 2,891,180 - -1.5 2,891,182 - +0.7 2,891,184 + -3.6 2,891,186 + -2.1 2,891,187 - -1.5 2,891,195 - -0.9 2,891,195 - -0.2 2,891,240 - -0.5 2,891,381 - -3.6 2,891,430 + +0.9 2,891,438 + -1.7 2,891,446 - -1.4 2,891,456 + -1.7 2,891,461 + +0.2 2,891,466 + -1.7 2,891,500 + +0.6 2,891,506 - +1.1 2,891,511 + BWI76_RS13865 0.11 +1.1 2,891,627 - BWI76_RS13865 0.24 +1.9 2,891,628 - BWI76_RS13865 0.24 +0.9 2,891,637 + BWI76_RS13865 0.25 -0.6 2,891,645 - BWI76_RS13865 0.26 -0.5 2,891,653 - BWI76_RS13865 0.27 +0.1 2,891,701 - BWI76_RS13865 0.32 +1.4 2,891,723 - BWI76_RS13865 0.34 -0.1 2,891,783 + BWI76_RS13865 0.41 +0.3 2,891,858 + BWI76_RS13865 0.49 +0.1 2,892,000 - BWI76_RS13865 0.65 +0.5 2,892,004 - BWI76_RS13865 0.65 +1.4 2,892,015 - BWI76_RS13865 0.67 +0.8 2,892,015 - BWI76_RS13865 0.67 -0.6 2,892,058 - BWI76_RS13865 0.71 -1.9 2,892,115 - BWI76_RS13865 0.78 -2.2 2,892,115 - BWI76_RS13865 0.78 -1.5 2,892,171 + BWI76_RS13865 0.84 -1.2
Or see this region's nucleotide sequence