Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12270 and BWI76_RS12275 are separated by 38 nucleotides BWI76_RS12275 and BWI76_RS12280 are separated by 3 nucleotides BWI76_RS12280 and BWI76_RS12285 are separated by 1 nucleotides BWI76_RS12285 and BWI76_RS12290 are separated by 1 nucleotides
BWI76_RS12270: BWI76_RS12270 - hypothetical protein, at 2,564,473 to 2,565,609
_RS12270
BWI76_RS12275: BWI76_RS12275 - hypothetical protein, at 2,565,648 to 2,565,857
_RS12275
BWI76_RS12280: BWI76_RS12280 - hypothetical protein, at 2,565,861 to 2,566,271
_RS12280
BWI76_RS12285: BWI76_RS12285 - hypothetical protein, at 2,566,273 to 2,566,656
_RS12285
BWI76_RS12290: BWI76_RS12290 - hypothetical protein, at 2,566,658 to 2,567,209
_RS12290
Position (kb)
2565
2566 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2564.656 kb on - strand, within BWI76_RS12270 at 2564.889 kb on + strand at 2564.902 kb on + strand, within BWI76_RS12270 at 2565.276 kb on + strand, within BWI76_RS12270 at 2565.387 kb on - strand, within BWI76_RS12270 at 2565.555 kb on + strand at 2565.724 kb on + strand, within BWI76_RS12275 at 2565.732 kb on - strand, within BWI76_RS12275 at 2565.801 kb on - strand, within BWI76_RS12275 at 2565.805 kb on + strand, within BWI76_RS12275 at 2565.813 kb on - strand, within BWI76_RS12275 at 2565.816 kb on - strand, within BWI76_RS12275 at 2565.917 kb on - strand, within BWI76_RS12280 at 2565.970 kb on + strand, within BWI76_RS12280 at 2565.978 kb on - strand, within BWI76_RS12280 at 2565.992 kb on + strand, within BWI76_RS12280 at 2566.222 kb on - strand, within BWI76_RS12280 at 2566.356 kb on + strand, within BWI76_RS12285 at 2566.435 kb on + strand, within BWI76_RS12285 at 2566.641 kb on + strand at 2566.713 kb on + strand at 2566.768 kb on + strand, within BWI76_RS12290 at 2566.810 kb on - strand, within BWI76_RS12290
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 2,564,656 - BWI76_RS12270 0.16 -0.2 2,564,889 + +2.6 2,564,902 + BWI76_RS12270 0.38 -0.5 2,565,276 + BWI76_RS12270 0.71 -4.6 2,565,387 - BWI76_RS12270 0.80 -2.8 2,565,555 + +0.2 2,565,724 + BWI76_RS12275 0.36 -3.8 2,565,732 - BWI76_RS12275 0.40 -0.6 2,565,801 - BWI76_RS12275 0.73 -1.0 2,565,805 + BWI76_RS12275 0.75 -0.9 2,565,813 - BWI76_RS12275 0.79 -0.2 2,565,816 - BWI76_RS12275 0.80 -0.4 2,565,917 - BWI76_RS12280 0.14 -0.8 2,565,970 + BWI76_RS12280 0.27 +0.1 2,565,978 - BWI76_RS12280 0.28 -2.2 2,565,992 + BWI76_RS12280 0.32 -3.7 2,566,222 - BWI76_RS12280 0.88 -1.9 2,566,356 + BWI76_RS12285 0.22 -2.6 2,566,435 + BWI76_RS12285 0.42 -1.2 2,566,641 + +1.0 2,566,713 + -2.5 2,566,768 + BWI76_RS12290 0.20 -4.1 2,566,810 - BWI76_RS12290 0.28 +0.9
Or see this region's nucleotide sequence