Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09960

Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09950 and BWI76_RS09955 are separated by 65 nucleotidesBWI76_RS09955 and BWI76_RS09960 are separated by 69 nucleotidesBWI76_RS09960 and BWI76_RS09965 are separated by 185 nucleotidesBWI76_RS09965 and BWI76_RS09970 are separated by 95 nucleotides BWI76_RS09950: BWI76_RS09950 - ribosome modulation factor, at 2,094,910 to 2,095,077 _RS09950 BWI76_RS09955: BWI76_RS09955 - beta-hydroxydecanoyl-ACP dehydratase, at 2,095,143 to 2,095,661 _RS09955 BWI76_RS09960: BWI76_RS09960 - Lon protease, at 2,095,731 to 2,097,488 _RS09960 BWI76_RS09965: BWI76_RS09965 - macrodomain Ter protein, at 2,097,674 to 2,098,126 _RS09965 BWI76_RS09970: BWI76_RS09970 - porin OmpA, at 2,098,222 to 2,099,292 _RS09970 Position (kb) 2095 2096 2097 2098Strain fitness (log2 ratio) -2 -1 0 1at 2095.070 kb on - strandat 2096.165 kb on - strand, within BWI76_RS09960at 2096.259 kb on - strand, within BWI76_RS09960at 2097.051 kb on + strand, within BWI76_RS09960at 2098.178 kb on - strandat 2098.290 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
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2,095,070 - +0.4
2,096,165 - BWI76_RS09960 0.25 -0.8
2,096,259 - BWI76_RS09960 0.30 -1.6
2,097,051 + BWI76_RS09960 0.75 -1.0
2,098,178 - -2.1
2,098,290 - -2.4

Or see this region's nucleotide sequence