Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09465 and BWI76_RS09470 are separated by 10 nucleotides BWI76_RS09470 and BWI76_RS09475 overlap by 4 nucleotides BWI76_RS09475 and BWI76_RS09480 are separated by 57 nucleotides
BWI76_RS09465: BWI76_RS09465 - polyamine ABC transporter ATP-binding protein, at 1,981,746 to 1,982,924
_RS09465
BWI76_RS09470: BWI76_RS09470 - putrescine ABC transporter permease, at 1,982,935 to 1,983,888
_RS09470
BWI76_RS09475: BWI76_RS09475 - putrescine ABC transporter permease PotI, at 1,983,885 to 1,984,730
_RS09475
BWI76_RS09480: BWI76_RS09480 - hypothetical protein, at 1,984,788 to 1,985,276
_RS09480
Position (kb) 1982
1983
1984 Strain fitness (log2 ratio) -2
-1
0
1 at 1982.027 kb on - strand, within BWI76_RS09465 at 1982.245 kb on - strand, within BWI76_RS09465 at 1982.359 kb on - strand, within BWI76_RS09465 at 1982.535 kb on - strand, within BWI76_RS09465 at 1982.595 kb on + strand, within BWI76_RS09465 at 1982.603 kb on - strand, within BWI76_RS09465 at 1982.603 kb on - strand, within BWI76_RS09465 at 1982.613 kb on - strand, within BWI76_RS09465 at 1982.724 kb on + strand at 1982.869 kb on - strand at 1983.548 kb on - strand, within BWI76_RS09470 at 1983.640 kb on - strand, within BWI76_RS09470 at 1983.739 kb on + strand, within BWI76_RS09470 at 1983.802 kb on - strand at 1984.331 kb on + strand, within BWI76_RS09475 at 1984.339 kb on - strand, within BWI76_RS09475 at 1984.378 kb on + strand, within BWI76_RS09475 at 1984.495 kb on + strand, within BWI76_RS09475 at 1984.503 kb on - strand, within BWI76_RS09475 at 1984.710 kb on - strand at 1984.777 kb on - strand at 1984.847 kb on - strand, within BWI76_RS09480 at 1984.859 kb on + strand, within BWI76_RS09480
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 1,982,027 - BWI76_RS09465 0.24 -0.4 1,982,245 - BWI76_RS09465 0.42 +1.3 1,982,359 - BWI76_RS09465 0.52 +0.4 1,982,535 - BWI76_RS09465 0.67 +0.0 1,982,595 + BWI76_RS09465 0.72 +0.2 1,982,603 - BWI76_RS09465 0.73 +1.2 1,982,603 - BWI76_RS09465 0.73 -0.1 1,982,613 - BWI76_RS09465 0.74 +1.3 1,982,724 + +1.2 1,982,869 - -0.4 1,983,548 - BWI76_RS09470 0.64 -2.7 1,983,640 - BWI76_RS09470 0.74 -2.7 1,983,739 + BWI76_RS09470 0.84 -1.0 1,983,802 - -1.8 1,984,331 + BWI76_RS09475 0.53 -1.1 1,984,339 - BWI76_RS09475 0.54 +0.7 1,984,378 + BWI76_RS09475 0.58 -0.1 1,984,495 + BWI76_RS09475 0.72 +0.4 1,984,503 - BWI76_RS09475 0.73 +0.7 1,984,710 - -0.7 1,984,777 - -0.0 1,984,847 - BWI76_RS09480 0.12 -0.2 1,984,859 + BWI76_RS09480 0.15 -0.1
Or see this region's nucleotide sequence