Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07355 and BWI76_RS07360 are separated by 141 nucleotides BWI76_RS07360 and BWI76_RS07365 are separated by 153 nucleotides
BWI76_RS07355: BWI76_RS07355 - TonB-dependent siderophore receptor, at 1,549,980 to 1,552,169
_RS07355
BWI76_RS07360: BWI76_RS07360 - phenylalanine transporter, at 1,552,311 to 1,553,687
_RS07360
BWI76_RS07365: BWI76_RS07365 - MFS transporter, at 1,553,841 to 1,555,082
_RS07365
Position (kb)
1552
1553
1554 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1551.319 kb on + strand, within BWI76_RS07355 at 1551.672 kb on + strand, within BWI76_RS07355 at 1551.820 kb on + strand, within BWI76_RS07355 at 1551.869 kb on + strand, within BWI76_RS07355 at 1551.879 kb on + strand, within BWI76_RS07355 at 1551.887 kb on - strand, within BWI76_RS07355 at 1552.103 kb on + strand at 1552.268 kb on + strand at 1552.270 kb on - strand at 1552.294 kb on + strand at 1552.311 kb on - strand at 1552.335 kb on - strand at 1552.483 kb on + strand, within BWI76_RS07360 at 1552.538 kb on + strand, within BWI76_RS07360 at 1552.795 kb on + strand, within BWI76_RS07360 at 1552.805 kb on + strand, within BWI76_RS07360 at 1552.805 kb on + strand, within BWI76_RS07360 at 1553.106 kb on + strand, within BWI76_RS07360 at 1553.338 kb on - strand, within BWI76_RS07360 at 1553.460 kb on - strand, within BWI76_RS07360 at 1553.563 kb on + strand at 1553.660 kb on + strand at 1553.962 kb on + strand at 1554.123 kb on + strand, within BWI76_RS07365 at 1554.185 kb on - strand, within BWI76_RS07365 at 1554.190 kb on + strand, within BWI76_RS07365 at 1554.190 kb on + strand, within BWI76_RS07365 at 1554.190 kb on + strand, within BWI76_RS07365 at 1554.190 kb on + strand, within BWI76_RS07365 at 1554.198 kb on - strand, within BWI76_RS07365 at 1554.248 kb on - strand, within BWI76_RS07365 at 1554.251 kb on + strand at 1554.261 kb on + strand, within BWI76_RS07365 at 1554.472 kb on - strand, within BWI76_RS07365
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 1,551,319 + BWI76_RS07355 0.61 +0.7 1,551,672 + BWI76_RS07355 0.77 -0.1 1,551,820 + BWI76_RS07355 0.84 -0.4 1,551,869 + BWI76_RS07355 0.86 +0.7 1,551,879 + BWI76_RS07355 0.87 -3.0 1,551,887 - BWI76_RS07355 0.87 -1.6 1,552,103 + -2.2 1,552,268 + -0.1 1,552,270 - +2.0 1,552,294 + +0.8 1,552,311 - +0.4 1,552,335 - +3.8 1,552,483 + BWI76_RS07360 0.12 -1.5 1,552,538 + BWI76_RS07360 0.16 -0.5 1,552,795 + BWI76_RS07360 0.35 +0.5 1,552,805 + BWI76_RS07360 0.36 -1.2 1,552,805 + BWI76_RS07360 0.36 -0.2 1,553,106 + BWI76_RS07360 0.58 -2.0 1,553,338 - BWI76_RS07360 0.75 -0.2 1,553,460 - BWI76_RS07360 0.83 -1.4 1,553,563 + -2.2 1,553,660 + -0.8 1,553,962 + +1.0 1,554,123 + BWI76_RS07365 0.23 -0.7 1,554,185 - BWI76_RS07365 0.28 +2.5 1,554,190 + BWI76_RS07365 0.28 -2.1 1,554,190 + BWI76_RS07365 0.28 -0.3 1,554,190 + BWI76_RS07365 0.28 -0.6 1,554,190 + BWI76_RS07365 0.28 +0.4 1,554,198 - BWI76_RS07365 0.29 -0.8 1,554,248 - BWI76_RS07365 0.33 -1.4 1,554,251 + -0.4 1,554,261 + BWI76_RS07365 0.34 +0.8 1,554,472 - BWI76_RS07365 0.51 -0.1
Or see this region's nucleotide sequence