Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05310 and BWI76_RS05315 are separated by 15 nucleotides BWI76_RS05315 and BWI76_RS05320 overlap by 14 nucleotides BWI76_RS05320 and BWI76_RS05325 are separated by 196 nucleotides BWI76_RS05325 and BWI76_RS05330 overlap by 4 nucleotides
BWI76_RS05310: BWI76_RS05310 - tRNA(Ile)-lysidine synthetase, at 1,120,373 to 1,121,680
_RS05310
BWI76_RS05315: BWI76_RS05315 - Rho-binding antiterminator, at 1,121,696 to 1,121,956
_RS05315
BWI76_RS05320: BWI76_RS05320 - hypothetical protein, at 1,121,943 to 1,122,143
_RS05320
BWI76_RS05325: BWI76_RS05325 - hypothetical protein, at 1,122,340 to 1,122,885
_RS05325
BWI76_RS05330: BWI76_RS05330 - aminoacyl-tRNA hydrolase, at 1,122,882 to 1,123,295
_RS05330
Position (kb) 1121
1122 Strain fitness (log2 ratio) -1
0
1 at 1121.826 kb on - strand, within BWI76_RS05315 at 1121.876 kb on + strand, within BWI76_RS05315 at 1121.876 kb on + strand, within BWI76_RS05315 at 1122.193 kb on - strand at 1122.206 kb on - strand at 1122.219 kb on + strand at 1122.463 kb on - strand, within BWI76_RS05325 at 1122.595 kb on - strand, within BWI76_RS05325 at 1122.743 kb on - strand, within BWI76_RS05325 at 1122.754 kb on - strand, within BWI76_RS05325 at 1122.762 kb on + strand, within BWI76_RS05325 at 1122.813 kb on - strand, within BWI76_RS05325 at 1122.813 kb on - strand, within BWI76_RS05325
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 1,121,826 - BWI76_RS05315 0.50 +0.0 1,121,876 + BWI76_RS05315 0.69 -1.2 1,121,876 + BWI76_RS05315 0.69 -1.6 1,122,193 - +0.6 1,122,206 - +0.7 1,122,219 + +0.5 1,122,463 - BWI76_RS05325 0.23 +0.1 1,122,595 - BWI76_RS05325 0.47 +1.1 1,122,743 - BWI76_RS05325 0.74 -0.9 1,122,754 - BWI76_RS05325 0.76 +0.9 1,122,762 + BWI76_RS05325 0.77 -0.1 1,122,813 - BWI76_RS05325 0.87 -1.4 1,122,813 - BWI76_RS05325 0.87 -1.1
Or see this region's nucleotide sequence