Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03575 and BWI76_RS03580 are separated by 25 nucleotides BWI76_RS03580 and BWI76_RS03585 are separated by 15 nucleotides BWI76_RS03585 and BWI76_RS03590 are separated by 26 nucleotides
BWI76_RS03575: BWI76_RS03575 - choline trimethylamine-lyase, at 739,454 to 742,840
_RS03575
BWI76_RS03580: BWI76_RS03580 - iron-containing alcohol dehydrogenase, at 742,866 to 744,008
_RS03580
BWI76_RS03585: BWI76_RS03585 - ethanolamine utilization protein EutN, at 744,024 to 744,281
_RS03585
BWI76_RS03590: BWI76_RS03590 - acetaldehyde dehydrogenase (acetylating), at 744,308 to 745,909
_RS03590
Position (kb) 742
743
744
745 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 741.997 kb on - strand, within BWI76_RS03575 at 742.279 kb on - strand, within BWI76_RS03575 at 742.322 kb on - strand, within BWI76_RS03575 at 742.612 kb on + strand at 742.819 kb on + strand at 742.819 kb on + strand at 742.827 kb on - strand at 742.827 kb on - strand at 742.841 kb on + strand at 742.841 kb on + strand at 742.841 kb on + strand at 742.888 kb on + strand at 743.040 kb on + strand, within BWI76_RS03580 at 743.073 kb on - strand, within BWI76_RS03580 at 743.102 kb on - strand, within BWI76_RS03580 at 743.292 kb on + strand, within BWI76_RS03580 at 743.655 kb on + strand, within BWI76_RS03580 at 743.685 kb on + strand, within BWI76_RS03580 at 743.858 kb on + strand, within BWI76_RS03580 at 744.070 kb on + strand, within BWI76_RS03585 at 744.070 kb on + strand, within BWI76_RS03585 at 744.070 kb on + strand, within BWI76_RS03585 at 744.120 kb on + strand, within BWI76_RS03585 at 744.617 kb on - strand, within BWI76_RS03590 at 744.653 kb on + strand, within BWI76_RS03590 at 744.653 kb on + strand, within BWI76_RS03590 at 744.658 kb on + strand, within BWI76_RS03590 at 744.779 kb on + strand, within BWI76_RS03590 at 744.787 kb on + strand, within BWI76_RS03590 at 744.937 kb on + strand, within BWI76_RS03590 at 744.984 kb on - strand, within BWI76_RS03590
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 741,997 - BWI76_RS03575 0.75 -3.7 742,279 - BWI76_RS03575 0.83 +0.5 742,322 - BWI76_RS03575 0.85 -0.9 742,612 + -1.5 742,819 + +1.6 742,819 + -0.4 742,827 - +0.0 742,827 - +0.7 742,841 + +0.5 742,841 + +0.0 742,841 + +0.7 742,888 + -0.1 743,040 + BWI76_RS03580 0.15 +0.7 743,073 - BWI76_RS03580 0.18 +0.1 743,102 - BWI76_RS03580 0.21 -2.0 743,292 + BWI76_RS03580 0.37 +0.2 743,655 + BWI76_RS03580 0.69 +1.2 743,685 + BWI76_RS03580 0.72 +1.4 743,858 + BWI76_RS03580 0.87 +0.6 744,070 + BWI76_RS03585 0.18 +1.0 744,070 + BWI76_RS03585 0.18 +0.9 744,070 + BWI76_RS03585 0.18 +0.6 744,120 + BWI76_RS03585 0.37 +0.5 744,617 - BWI76_RS03590 0.19 -0.1 744,653 + BWI76_RS03590 0.22 +0.2 744,653 + BWI76_RS03590 0.22 -0.4 744,658 + BWI76_RS03590 0.22 +0.2 744,779 + BWI76_RS03590 0.29 -0.5 744,787 + BWI76_RS03590 0.30 -0.3 744,937 + BWI76_RS03590 0.39 -0.5 744,984 - BWI76_RS03590 0.42 +1.5
Or see this region's nucleotide sequence