Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00870 and BWI76_RS00875 are separated by 196 nucleotides BWI76_RS00875 and BWI76_RS00880 are separated by 15 nucleotides BWI76_RS00880 and BWI76_RS00885 are separated by 46 nucleotides
BWI76_RS00870: BWI76_RS00870 - NAD(P)(+) transhydrogenase, at 179,330 to 180,730
_RS00870
BWI76_RS00875: BWI76_RS00875 - DNA-binding transcriptional regulator FabR, at 180,927 to 181,562
_RS00875
BWI76_RS00880: BWI76_RS00880 - membrane protein, at 181,578 to 181,937
_RS00880
BWI76_RS00885: BWI76_RS00885 - tRNA (uridine(54)-C5)-methyltransferase TrmA, at 181,984 to 183,084
_RS00885
Position (kb) 180
181
182 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 180.794 kb on - strand at 180.883 kb on - strand at 180.885 kb on + strand at 180.915 kb on + strand at 180.923 kb on - strand at 181.021 kb on + strand, within BWI76_RS00875 at 181.021 kb on + strand, within BWI76_RS00875 at 181.021 kb on + strand, within BWI76_RS00875 at 181.109 kb on + strand, within BWI76_RS00875 at 181.129 kb on + strand, within BWI76_RS00875 at 181.294 kb on + strand, within BWI76_RS00875 at 181.383 kb on + strand, within BWI76_RS00875 at 181.388 kb on + strand, within BWI76_RS00875 at 181.396 kb on - strand, within BWI76_RS00875 at 181.444 kb on + strand, within BWI76_RS00875 at 181.586 kb on - strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.595 kb on + strand at 181.595 kb on + strand at 181.598 kb on - strand at 181.603 kb on - strand at 181.659 kb on + strand, within BWI76_RS00880 at 181.661 kb on + strand, within BWI76_RS00880 at 181.661 kb on + strand, within BWI76_RS00880 at 181.886 kb on + strand, within BWI76_RS00880 at 182.436 kb on + strand, within BWI76_RS00885 at 182.439 kb on - strand, within BWI76_RS00885
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 180,794 - -2.9 180,883 - +2.8 180,885 + -1.0 180,915 + -0.5 180,923 - -2.8 181,021 + BWI76_RS00875 0.15 -3.5 181,021 + BWI76_RS00875 0.15 -3.3 181,021 + BWI76_RS00875 0.15 +1.5 181,109 + BWI76_RS00875 0.29 +1.5 181,129 + BWI76_RS00875 0.32 -3.3 181,294 + BWI76_RS00875 0.58 -2.1 181,383 + BWI76_RS00875 0.72 -1.3 181,388 + BWI76_RS00875 0.72 -2.3 181,396 - BWI76_RS00875 0.74 +1.5 181,444 + BWI76_RS00875 0.81 -2.5 181,586 - +0.5 181,590 + -0.3 181,590 + +1.5 181,590 + -0.5 181,590 + -1.6 181,590 + +2.1 181,595 + -0.4 181,595 + +0.4 181,598 - +2.8 181,603 - -0.8 181,659 + BWI76_RS00880 0.23 -0.0 181,661 + BWI76_RS00880 0.23 +0.5 181,661 + BWI76_RS00880 0.23 -1.1 181,886 + BWI76_RS00880 0.86 -1.4 182,436 + BWI76_RS00885 0.41 -0.9 182,439 - BWI76_RS00885 0.41 +0.4
Or see this region's nucleotide sequence