Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25645 and BWI76_RS25650 are separated by 3 nucleotides BWI76_RS25650 and BWI76_RS25655 are separated by 4 nucleotides BWI76_RS25655 and BWI76_RS25660 are separated by 27 nucleotides BWI76_RS25660 and BWI76_RS25665 overlap by 29 nucleotides
BWI76_RS25645: BWI76_RS25645 - shikimate dehydrogenase, at 5,326,644 to 5,327,462
_RS25645
BWI76_RS25650: BWI76_RS25650 - L-threonylcarbamoyladenylate synthase type 1 TsaC, at 5,327,466 to 5,328,038
_RS25650
BWI76_RS25655: BWI76_RS25655 - DNA topoisomerase type IA zn finger domain-containing protein, at 5,328,043 to 5,328,585
_RS25655
BWI76_RS25660: BWI76_RS25660 - hypothetical protein, at 5,328,613 to 5,329,086
_RS25660
BWI76_RS25665: BWI76_RS25665 - DNA-processing protein DprA, at 5,329,058 to 5,330,182
_RS25665
Position (kb) 5328
5329 Strain fitness (log2 ratio) -1
0
1 at 5327.065 kb on - strand, within BWI76_RS25645 at 5327.139 kb on + strand, within BWI76_RS25645 at 5327.147 kb on + strand, within BWI76_RS25645 at 5327.147 kb on + strand, within BWI76_RS25645 at 5327.147 kb on - strand, within BWI76_RS25645 at 5327.193 kb on - strand, within BWI76_RS25645 at 5327.217 kb on + strand, within BWI76_RS25645 at 5327.217 kb on + strand, within BWI76_RS25645 at 5327.225 kb on - strand, within BWI76_RS25645 at 5327.301 kb on - strand, within BWI76_RS25645 at 5327.362 kb on - strand, within BWI76_RS25645 at 5328.405 kb on - strand, within BWI76_RS25655 at 5328.414 kb on - strand, within BWI76_RS25655 at 5328.470 kb on + strand at 5328.492 kb on - strand, within BWI76_RS25655 at 5328.520 kb on - strand, within BWI76_RS25655 at 5328.763 kb on + strand, within BWI76_RS25660 at 5328.866 kb on + strand, within BWI76_RS25660 at 5328.951 kb on + strand, within BWI76_RS25660 at 5329.015 kb on + strand, within BWI76_RS25660 at 5329.023 kb on - strand, within BWI76_RS25660 at 5329.055 kb on + strand at 5329.133 kb on - strand at 5329.140 kb on + strand at 5329.153 kb on - strand at 5329.215 kb on + strand, within BWI76_RS25665 at 5329.218 kb on - strand, within BWI76_RS25665 at 5329.224 kb on - strand, within BWI76_RS25665 at 5329.345 kb on - strand, within BWI76_RS25665 at 5329.401 kb on - strand, within BWI76_RS25665
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 5,327,065 - BWI76_RS25645 0.51 -1.0 5,327,139 + BWI76_RS25645 0.60 -0.4 5,327,147 + BWI76_RS25645 0.61 +0.7 5,327,147 + BWI76_RS25645 0.61 +0.5 5,327,147 - BWI76_RS25645 0.61 +1.7 5,327,193 - BWI76_RS25645 0.67 +0.4 5,327,217 + BWI76_RS25645 0.70 +1.1 5,327,217 + BWI76_RS25645 0.70 +0.1 5,327,225 - BWI76_RS25645 0.71 +0.1 5,327,301 - BWI76_RS25645 0.80 -1.2 5,327,362 - BWI76_RS25645 0.88 +0.8 5,328,405 - BWI76_RS25655 0.67 -0.4 5,328,414 - BWI76_RS25655 0.68 -1.2 5,328,470 + +0.1 5,328,492 - BWI76_RS25655 0.83 -1.6 5,328,520 - BWI76_RS25655 0.88 -0.2 5,328,763 + BWI76_RS25660 0.32 -0.0 5,328,866 + BWI76_RS25660 0.53 +0.2 5,328,951 + BWI76_RS25660 0.71 +1.6 5,329,015 + BWI76_RS25660 0.85 -1.3 5,329,023 - BWI76_RS25660 0.86 -0.9 5,329,055 + +0.3 5,329,133 - +0.8 5,329,140 + +0.1 5,329,153 - -0.1 5,329,215 + BWI76_RS25665 0.14 +1.0 5,329,218 - BWI76_RS25665 0.14 -1.5 5,329,224 - BWI76_RS25665 0.15 +0.2 5,329,345 - BWI76_RS25665 0.26 +0.3 5,329,401 - BWI76_RS25665 0.30 +0.2
Or see this region's nucleotide sequence