Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18265 and BWI76_RS18270 are separated by 46 nucleotides BWI76_RS18270 and BWI76_RS18275 are separated by 371 nucleotides BWI76_RS18275 and BWI76_RS18280 are separated by 47 nucleotides
BWI76_RS18265: BWI76_RS18265 - MFS transporter, at 3,795,745 to 3,797,307
_RS18265
BWI76_RS18270: BWI76_RS18270 - hypothetical protein, at 3,797,354 to 3,797,605
_RS18270
BWI76_RS18275: BWI76_RS18275 - MFS transporter, at 3,797,977 to 3,799,401
_RS18275
BWI76_RS18280: BWI76_RS18280 - hypothetical protein, at 3,799,449 to 3,800,087
_RS18280
Position (kb)
3797
3798
3799
3800 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3797.154 kb on + strand at 3797.431 kb on + strand, within BWI76_RS18270 at 3797.761 kb on + strand at 3797.922 kb on + strand at 3797.922 kb on + strand at 3797.925 kb on - strand at 3797.925 kb on - strand at 3797.930 kb on - strand at 3797.949 kb on - strand at 3798.093 kb on - strand at 3798.299 kb on - strand, within BWI76_RS18275 at 3798.398 kb on + strand, within BWI76_RS18275 at 3798.403 kb on + strand at 3798.411 kb on - strand, within BWI76_RS18275 at 3798.562 kb on + strand, within BWI76_RS18275 at 3798.570 kb on - strand, within BWI76_RS18275 at 3798.701 kb on + strand, within BWI76_RS18275 at 3798.787 kb on - strand, within BWI76_RS18275 at 3799.023 kb on - strand, within BWI76_RS18275 at 3799.181 kb on - strand, within BWI76_RS18275 at 3799.184 kb on + strand, within BWI76_RS18275 at 3799.184 kb on + strand, within BWI76_RS18275 at 3799.229 kb on + strand, within BWI76_RS18275 at 3799.259 kb on + strand at 3799.351 kb on + strand at 3799.369 kb on + strand at 3799.411 kb on - strand at 3799.466 kb on - strand at 3799.548 kb on - strand, within BWI76_RS18280 at 3799.635 kb on + strand, within BWI76_RS18280 at 3799.637 kb on + strand, within BWI76_RS18280 at 3799.730 kb on + strand, within BWI76_RS18280 at 3799.745 kb on - strand, within BWI76_RS18280 at 3799.771 kb on + strand, within BWI76_RS18280 at 3799.941 kb on + strand, within BWI76_RS18280 at 3799.949 kb on - strand, within BWI76_RS18280 at 3799.961 kb on - strand, within BWI76_RS18280 at 3800.096 kb on + strand at 3800.098 kb on + strand at 3800.098 kb on + strand at 3800.098 kb on + strand at 3800.098 kb on + strand at 3800.106 kb on - strand at 3800.106 kb on - strand at 3800.106 kb on - strand at 3800.106 kb on - strand at 3800.106 kb on - strand at 3800.106 kb on - strand at 3800.106 kb on - strand at 3800.144 kb on - strand at 3800.234 kb on - strand at 3800.254 kb on - strand at 3800.257 kb on - strand at 3800.257 kb on - strand at 3800.266 kb on + strand at 3800.266 kb on + strand at 3800.266 kb on + strand at 3800.267 kb on - strand at 3800.271 kb on + strand at 3800.272 kb on - strand at 3800.274 kb on - strand at 3800.275 kb on + strand at 3800.275 kb on + strand at 3800.287 kb on - strand at 3800.306 kb on - strand at 3800.306 kb on - strand at 3800.312 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 3,797,154 + +0.8 3,797,431 + BWI76_RS18270 0.31 +0.8 3,797,761 + +0.8 3,797,922 + +0.5 3,797,922 + -0.2 3,797,925 - -0.1 3,797,925 - +1.1 3,797,930 - -1.7 3,797,949 - -0.2 3,798,093 - -0.6 3,798,299 - BWI76_RS18275 0.23 +1.2 3,798,398 + BWI76_RS18275 0.30 -2.3 3,798,403 + -0.1 3,798,411 - BWI76_RS18275 0.30 -1.7 3,798,562 + BWI76_RS18275 0.41 -0.8 3,798,570 - BWI76_RS18275 0.42 -0.1 3,798,701 + BWI76_RS18275 0.51 -0.7 3,798,787 - BWI76_RS18275 0.57 -0.7 3,799,023 - BWI76_RS18275 0.73 +0.4 3,799,181 - BWI76_RS18275 0.84 -1.7 3,799,184 + BWI76_RS18275 0.85 +0.5 3,799,184 + BWI76_RS18275 0.85 +0.1 3,799,229 + BWI76_RS18275 0.88 -3.3 3,799,259 + -0.4 3,799,351 + -1.8 3,799,369 + -2.3 3,799,411 - -3.2 3,799,466 - +0.3 3,799,548 - BWI76_RS18280 0.15 -0.9 3,799,635 + BWI76_RS18280 0.29 +0.4 3,799,637 + BWI76_RS18280 0.29 +0.5 3,799,730 + BWI76_RS18280 0.44 +0.5 3,799,745 - BWI76_RS18280 0.46 +0.9 3,799,771 + BWI76_RS18280 0.50 +0.3 3,799,941 + BWI76_RS18280 0.77 +1.8 3,799,949 - BWI76_RS18280 0.78 +1.0 3,799,961 - BWI76_RS18280 0.80 +0.1 3,800,096 + +1.5 3,800,098 + -1.4 3,800,098 + -1.1 3,800,098 + -0.1 3,800,098 + +1.7 3,800,106 - +0.6 3,800,106 - -0.0 3,800,106 - -0.2 3,800,106 - -0.3 3,800,106 - +0.6 3,800,106 - -0.1 3,800,106 - +1.7 3,800,144 - -0.8 3,800,234 - +1.1 3,800,254 - +1.1 3,800,257 - +1.0 3,800,257 - +0.4 3,800,266 + +0.1 3,800,266 + -0.1 3,800,266 + -0.2 3,800,267 - +0.8 3,800,271 + +0.6 3,800,272 - -0.5 3,800,274 - +1.1 3,800,275 + -0.0 3,800,275 + +0.2 3,800,287 - +0.4 3,800,306 - -1.4 3,800,306 - +0.7 3,800,312 + -1.1
Or see this region's nucleotide sequence