Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17805 and BWI76_RS17810 are separated by 43 nucleotides BWI76_RS17810 and BWI76_RS17815 are separated by 67 nucleotides BWI76_RS17815 and BWI76_RS17820 are separated by 132 nucleotides BWI76_RS17820 and BWI76_RS17825 are separated by 164 nucleotides
BWI76_RS17805: BWI76_RS17805 - membrane protein, at 3,701,023 to 3,701,607
_RS17805
BWI76_RS17810: BWI76_RS17810 - tRNA N6-adenosine(37)-N6-threonylcarbamoyltransferase complex dimerization subunit TsaB, at 3,701,651 to 3,702,346
_RS17810
BWI76_RS17815: BWI76_RS17815 - ATP-dependent helicase, at 3,702,414 to 3,704,324
_RS17815
BWI76_RS17820: BWI76_RS17820 - hypothetical protein, at 3,704,457 to 3,704,801
_RS17820
BWI76_RS17825: BWI76_RS17825 - alginate lyase, at 3,704,966 to 3,706,105
_RS17825
Position (kb) 3702
3703
3704
3705 Strain fitness (log2 ratio) -2
-1
0
1 at 3701.461 kb on - strand, within BWI76_RS17805 at 3702.456 kb on + strand at 3702.587 kb on - strand at 3702.590 kb on + strand at 3702.598 kb on - strand at 3702.866 kb on - strand, within BWI76_RS17815 at 3702.902 kb on - strand, within BWI76_RS17815 at 3702.917 kb on + strand, within BWI76_RS17815 at 3702.925 kb on - strand, within BWI76_RS17815 at 3702.925 kb on - strand, within BWI76_RS17815 at 3702.925 kb on - strand, within BWI76_RS17815 at 3702.925 kb on - strand, within BWI76_RS17815 at 3702.948 kb on - strand, within BWI76_RS17815 at 3702.990 kb on - strand, within BWI76_RS17815 at 3703.026 kb on - strand, within BWI76_RS17815 at 3703.037 kb on - strand, within BWI76_RS17815 at 3703.093 kb on - strand, within BWI76_RS17815 at 3703.847 kb on - strand, within BWI76_RS17815 at 3703.980 kb on - strand, within BWI76_RS17815 at 3703.993 kb on - strand, within BWI76_RS17815 at 3703.993 kb on - strand, within BWI76_RS17815 at 3704.266 kb on + strand at 3704.530 kb on - strand, within BWI76_RS17820 at 3704.724 kb on + strand, within BWI76_RS17820 at 3704.796 kb on + strand at 3704.796 kb on + strand at 3704.804 kb on - strand at 3704.804 kb on - strand at 3704.828 kb on - strand at 3705.108 kb on + strand, within BWI76_RS17825 at 3705.136 kb on - strand, within BWI76_RS17825
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 3,701,461 - BWI76_RS17805 0.75 +1.3 3,702,456 + +0.9 3,702,587 - +0.8 3,702,590 + +0.8 3,702,598 - +0.5 3,702,866 - BWI76_RS17815 0.24 +0.0 3,702,902 - BWI76_RS17815 0.26 +0.5 3,702,917 + BWI76_RS17815 0.26 -1.0 3,702,925 - BWI76_RS17815 0.27 +0.6 3,702,925 - BWI76_RS17815 0.27 +0.7 3,702,925 - BWI76_RS17815 0.27 -1.7 3,702,925 - BWI76_RS17815 0.27 +0.3 3,702,948 - BWI76_RS17815 0.28 -1.0 3,702,990 - BWI76_RS17815 0.30 +0.6 3,703,026 - BWI76_RS17815 0.32 +0.4 3,703,037 - BWI76_RS17815 0.33 +0.7 3,703,093 - BWI76_RS17815 0.36 -2.0 3,703,847 - BWI76_RS17815 0.75 +0.2 3,703,980 - BWI76_RS17815 0.82 +0.2 3,703,993 - BWI76_RS17815 0.83 +1.2 3,703,993 - BWI76_RS17815 0.83 +1.1 3,704,266 + -1.1 3,704,530 - BWI76_RS17820 0.21 -1.5 3,704,724 + BWI76_RS17820 0.77 -0.1 3,704,796 + -0.3 3,704,796 + +0.6 3,704,804 - +0.2 3,704,804 - -0.0 3,704,828 - -0.1 3,705,108 + BWI76_RS17825 0.12 +0.6 3,705,136 - BWI76_RS17825 0.15 +0.1
Or see this region's nucleotide sequence