Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16035 and BWI76_RS16040 overlap by 1 nucleotides BWI76_RS16040 and BWI76_RS16045 are separated by 129 nucleotides BWI76_RS16045 and BWI76_RS16050 are separated by 2 nucleotides BWI76_RS16050 and BWI76_RS16055 overlap by 4 nucleotides
BWI76_RS16035: BWI76_RS16035 - biotin-independent malonate decarboxylase subunit beta, at 3,324,924 to 3,325,757
_RS16035
BWI76_RS16040: BWI76_RS16040 - biotin-independent malonate decarboxylase subunit gamma, at 3,325,757 to 3,326,557
_RS16040
BWI76_RS16045: BWI76_RS16045 - transporter, at 3,326,687 to 3,327,646
_RS16045
BWI76_RS16050: BWI76_RS16050 - malonate decarboxylase holo-ACP synthase, at 3,327,649 to 3,328,269
_RS16050
BWI76_RS16055: BWI76_RS16055 - malonate decarboxylase subunit epsilon, at 3,328,266 to 3,329,168
_RS16055
Position (kb) 3326
3327
3328 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3326.371 kb on + strand, within BWI76_RS16040 at 3326.379 kb on - strand, within BWI76_RS16040 at 3326.517 kb on + strand at 3326.525 kb on - strand at 3326.580 kb on - strand at 3326.582 kb on + strand at 3326.841 kb on - strand, within BWI76_RS16045 at 3327.330 kb on + strand, within BWI76_RS16045 at 3327.471 kb on + strand, within BWI76_RS16045 at 3327.735 kb on + strand, within BWI76_RS16050 at 3327.812 kb on - strand, within BWI76_RS16050 at 3327.883 kb on - strand, within BWI76_RS16050 at 3328.433 kb on - strand, within BWI76_RS16055 at 3328.607 kb on + strand, within BWI76_RS16055
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 3,326,371 + BWI76_RS16040 0.77 +0.2 3,326,379 - BWI76_RS16040 0.78 -2.9 3,326,517 + -0.1 3,326,525 - -0.7 3,326,580 - -0.7 3,326,582 + +0.2 3,326,841 - BWI76_RS16045 0.16 -1.3 3,327,330 + BWI76_RS16045 0.67 -2.1 3,327,471 + BWI76_RS16045 0.82 +0.9 3,327,735 + BWI76_RS16050 0.14 +0.1 3,327,812 - BWI76_RS16050 0.26 -1.2 3,327,883 - BWI76_RS16050 0.38 -0.3 3,328,433 - BWI76_RS16055 0.18 -0.3 3,328,607 + BWI76_RS16055 0.38 +0.3
Or see this region's nucleotide sequence