Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07385 and BWI76_RS07390 are separated by 180 nucleotides BWI76_RS07390 and BWI76_RS07395 are separated by 320 nucleotides BWI76_RS07395 and BWI76_RS07400 are separated by 6 nucleotides
BWI76_RS07385: BWI76_RS07385 - short-chain dehydrogenase/reductase SDR, at 1,557,857 to 1,558,606
_RS07385
BWI76_RS07390: BWI76_RS07390 - putative LysR-family transcriptional regulator, at 1,558,787 to 1,559,716
_RS07390
BWI76_RS07395: BWI76_RS07395 - LuxR family transcriptional regulator, at 1,560,037 to 1,560,672
_RS07395
BWI76_RS07400: BWI76_RS07400 - fimbrial protein, at 1,560,679 to 1,561,359
_RS07400
Position (kb)
1558
1559
1560 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1557.811 kb on - strand at 1557.816 kb on + strand at 1557.824 kb on - strand at 1557.939 kb on + strand at 1558.175 kb on + strand, within BWI76_RS07385 at 1558.394 kb on - strand, within BWI76_RS07385 at 1558.839 kb on + strand at 1558.883 kb on - strand, within BWI76_RS07390 at 1558.883 kb on - strand, within BWI76_RS07390 at 1558.946 kb on - strand at 1559.178 kb on + strand, within BWI76_RS07390 at 1559.178 kb on + strand, within BWI76_RS07390 at 1559.233 kb on - strand, within BWI76_RS07390 at 1559.243 kb on - strand, within BWI76_RS07390 at 1559.298 kb on + strand, within BWI76_RS07390 at 1559.298 kb on + strand, within BWI76_RS07390 at 1559.313 kb on - strand, within BWI76_RS07390 at 1559.321 kb on - strand, within BWI76_RS07390 at 1559.415 kb on - strand, within BWI76_RS07390 at 1559.451 kb on + strand, within BWI76_RS07390 at 1559.456 kb on + strand, within BWI76_RS07390 at 1559.456 kb on + strand, within BWI76_RS07390 at 1559.459 kb on - strand, within BWI76_RS07390 at 1559.464 kb on - strand, within BWI76_RS07390 at 1559.488 kb on + strand, within BWI76_RS07390 at 1559.488 kb on + strand, within BWI76_RS07390 at 1559.490 kb on - strand, within BWI76_RS07390 at 1559.495 kb on - strand, within BWI76_RS07390 at 1559.500 kb on - strand, within BWI76_RS07390 at 1559.512 kb on - strand, within BWI76_RS07390 at 1559.512 kb on - strand, within BWI76_RS07390 at 1559.524 kb on + strand, within BWI76_RS07390 at 1559.547 kb on - strand, within BWI76_RS07390 at 1559.592 kb on - strand, within BWI76_RS07390 at 1559.603 kb on + strand, within BWI76_RS07390 at 1559.628 kb on + strand at 1559.628 kb on + strand at 1559.628 kb on + strand at 1559.628 kb on + strand at 1559.628 kb on + strand at 1559.628 kb on + strand at 1559.628 kb on + strand at 1559.636 kb on - strand at 1559.636 kb on - strand at 1559.636 kb on - strand at 1559.660 kb on + strand at 1559.667 kb on - strand at 1559.675 kb on - strand at 1559.683 kb on + strand at 1559.703 kb on - strand at 1559.716 kb on + strand at 1559.718 kb on - strand at 1559.749 kb on + strand at 1559.803 kb on - strand at 1559.803 kb on - strand at 1560.047 kb on - strand at 1560.065 kb on - strand at 1560.084 kb on - strand at 1560.122 kb on + strand, within BWI76_RS07395 at 1560.227 kb on - strand, within BWI76_RS07395 at 1560.331 kb on + strand, within BWI76_RS07395 at 1560.331 kb on + strand, within BWI76_RS07395 at 1560.335 kb on + strand, within BWI76_RS07395 at 1560.339 kb on - strand, within BWI76_RS07395 at 1560.345 kb on + strand, within BWI76_RS07395 at 1560.345 kb on + strand, within BWI76_RS07395 at 1560.391 kb on + strand, within BWI76_RS07395 at 1560.391 kb on + strand, within BWI76_RS07395 at 1560.399 kb on + strand, within BWI76_RS07395 at 1560.399 kb on + strand, within BWI76_RS07395 at 1560.409 kb on + strand, within BWI76_RS07395 at 1560.417 kb on - strand, within BWI76_RS07395 at 1560.417 kb on - strand, within BWI76_RS07395 at 1560.417 kb on - strand, within BWI76_RS07395 at 1560.471 kb on + strand, within BWI76_RS07395 at 1560.471 kb on + strand, within BWI76_RS07395 at 1560.479 kb on - strand, within BWI76_RS07395 at 1560.480 kb on + strand, within BWI76_RS07395 at 1560.480 kb on + strand, within BWI76_RS07395 at 1560.480 kb on + strand, within BWI76_RS07395 at 1560.480 kb on + strand, within BWI76_RS07395 at 1560.480 kb on + strand, within BWI76_RS07395 at 1560.480 kb on + strand, within BWI76_RS07395 at 1560.480 kb on + strand, within BWI76_RS07395 at 1560.480 kb on + strand, within BWI76_RS07395 at 1560.480 kb on + strand, within BWI76_RS07395 at 1560.488 kb on - strand, within BWI76_RS07395 at 1560.488 kb on - strand, within BWI76_RS07395 at 1560.488 kb on - strand, within BWI76_RS07395 at 1560.488 kb on - strand, within BWI76_RS07395 at 1560.488 kb on - strand, within BWI76_RS07395 at 1560.516 kb on + strand, within BWI76_RS07395 at 1560.531 kb on - strand, within BWI76_RS07395 at 1560.540 kb on + strand, within BWI76_RS07395 at 1560.543 kb on - strand, within BWI76_RS07395 at 1560.585 kb on - strand, within BWI76_RS07395 at 1560.647 kb on + strand at 1560.659 kb on - strand at 1560.662 kb on + strand at 1560.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 1,557,811 - +0.3 1,557,816 + +1.3 1,557,824 - +1.9 1,557,939 + +0.9 1,558,175 + BWI76_RS07385 0.42 -0.7 1,558,394 - BWI76_RS07385 0.72 +0.6 1,558,839 + +0.2 1,558,883 - BWI76_RS07390 0.10 -3.1 1,558,883 - BWI76_RS07390 0.10 -2.1 1,558,946 - +0.9 1,559,178 + BWI76_RS07390 0.42 +1.4 1,559,178 + BWI76_RS07390 0.42 -0.0 1,559,233 - BWI76_RS07390 0.48 +0.2 1,559,243 - BWI76_RS07390 0.49 -0.6 1,559,298 + BWI76_RS07390 0.55 -0.5 1,559,298 + BWI76_RS07390 0.55 -1.9 1,559,313 - BWI76_RS07390 0.57 -2.8 1,559,321 - BWI76_RS07390 0.57 -1.0 1,559,415 - BWI76_RS07390 0.68 -1.2 1,559,451 + BWI76_RS07390 0.71 +0.9 1,559,456 + BWI76_RS07390 0.72 -1.1 1,559,456 + BWI76_RS07390 0.72 -0.7 1,559,459 - BWI76_RS07390 0.72 -0.4 1,559,464 - BWI76_RS07390 0.73 +0.9 1,559,488 + BWI76_RS07390 0.75 -0.6 1,559,488 + BWI76_RS07390 0.75 -2.1 1,559,490 - BWI76_RS07390 0.76 -0.7 1,559,495 - BWI76_RS07390 0.76 -1.2 1,559,500 - BWI76_RS07390 0.77 -1.9 1,559,512 - BWI76_RS07390 0.78 +1.0 1,559,512 - BWI76_RS07390 0.78 -0.7 1,559,524 + BWI76_RS07390 0.79 -0.9 1,559,547 - BWI76_RS07390 0.82 -1.1 1,559,592 - BWI76_RS07390 0.87 -3.3 1,559,603 + BWI76_RS07390 0.88 -0.6 1,559,628 + -0.2 1,559,628 + -1.4 1,559,628 + +0.3 1,559,628 + -0.7 1,559,628 + -0.1 1,559,628 + -1.4 1,559,628 + +0.3 1,559,636 - -0.1 1,559,636 - -0.5 1,559,636 - -1.1 1,559,660 + -1.6 1,559,667 - +0.9 1,559,675 - +1.0 1,559,683 + -0.7 1,559,703 - -3.6 1,559,716 + +0.3 1,559,718 - +1.2 1,559,749 + +0.9 1,559,803 - -0.3 1,559,803 - -1.7 1,560,047 - -2.7 1,560,065 - -1.9 1,560,084 - -0.1 1,560,122 + BWI76_RS07395 0.13 +0.9 1,560,227 - BWI76_RS07395 0.30 -1.7 1,560,331 + BWI76_RS07395 0.46 -0.0 1,560,331 + BWI76_RS07395 0.46 +1.9 1,560,335 + BWI76_RS07395 0.47 -0.1 1,560,339 - BWI76_RS07395 0.47 -1.4 1,560,345 + BWI76_RS07395 0.48 -2.3 1,560,345 + BWI76_RS07395 0.48 -0.2 1,560,391 + BWI76_RS07395 0.56 +0.5 1,560,391 + BWI76_RS07395 0.56 -0.7 1,560,399 + BWI76_RS07395 0.57 -0.7 1,560,399 + BWI76_RS07395 0.57 -2.6 1,560,409 + BWI76_RS07395 0.58 -0.8 1,560,417 - BWI76_RS07395 0.60 +0.9 1,560,417 - BWI76_RS07395 0.60 +1.1 1,560,417 - BWI76_RS07395 0.60 +0.9 1,560,471 + BWI76_RS07395 0.68 +1.7 1,560,471 + BWI76_RS07395 0.68 -0.1 1,560,479 - BWI76_RS07395 0.69 -1.1 1,560,480 + BWI76_RS07395 0.70 +0.7 1,560,480 + BWI76_RS07395 0.70 +0.5 1,560,480 + BWI76_RS07395 0.70 -1.1 1,560,480 + BWI76_RS07395 0.70 +0.9 1,560,480 + BWI76_RS07395 0.70 -2.1 1,560,480 + BWI76_RS07395 0.70 -0.4 1,560,480 + BWI76_RS07395 0.70 +0.9 1,560,480 + BWI76_RS07395 0.70 +0.7 1,560,480 + BWI76_RS07395 0.70 +0.3 1,560,488 - BWI76_RS07395 0.71 -2.4 1,560,488 - BWI76_RS07395 0.71 -0.7 1,560,488 - BWI76_RS07395 0.71 -1.7 1,560,488 - BWI76_RS07395 0.71 -4.3 1,560,488 - BWI76_RS07395 0.71 -0.8 1,560,516 + BWI76_RS07395 0.75 -0.1 1,560,531 - BWI76_RS07395 0.78 +1.6 1,560,540 + BWI76_RS07395 0.79 +0.3 1,560,543 - BWI76_RS07395 0.80 -3.1 1,560,585 - BWI76_RS07395 0.86 -1.9 1,560,647 + +0.9 1,560,659 - -2.1 1,560,662 + -1.7 1,560,711 - -0.1
Or see this region's nucleotide sequence