Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05220 and BWI76_RS05225 are separated by 205 nucleotides BWI76_RS05225 and BWI76_RS05230 are separated by 226 nucleotides BWI76_RS05230 and BWI76_RS05235 are separated by 15 nucleotides BWI76_RS05235 and BWI76_RS05240 overlap by 14 nucleotides
BWI76_RS05220: BWI76_RS05220 - UMP kinase, at 1,099,544 to 1,100,269
_RS05220
BWI76_RS05225: BWI76_RS05225 - ribosome-recycling factor, at 1,100,475 to 1,101,032
_RS05225
BWI76_RS05230: BWI76_RS05230 - 1-deoxy-D-xylulose-5-phosphate reductoisomerase, at 1,101,259 to 1,102,458
_RS05230
BWI76_RS05235: BWI76_RS05235 - hypothetical protein, at 1,102,474 to 1,102,714
_RS05235
BWI76_RS05240: BWI76_RS05240 - (2E,6E)-farnesyl- diphosphate-specific ditrans,polycis-undecaprenyl-diphosphate synthase, at 1,102,701 to 1,103,459
_RS05240
Position (kb) 1101
1102
1103 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1101.050 kb on + strand at 1101.058 kb on - strand at 1101.058 kb on - strand at 1102.485 kb on + strand at 1102.500 kb on + strand, within BWI76_RS05235
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 1,101,050 + -1.6 1,101,058 - +0.8 1,101,058 - -0.6 1,102,485 + +1.0 1,102,500 + BWI76_RS05235 0.11 -3.2
Or see this region's nucleotide sequence