Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04950 and BWI76_RS04955 are separated by 203 nucleotides BWI76_RS04955 and BWI76_RS04960 are separated by 50 nucleotides BWI76_RS04960 and BWI76_RS04965 are separated by 107 nucleotides
BWI76_RS04950: BWI76_RS04950 - membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family, at 1,038,990 to 1,041,380
_RS04950
BWI76_RS04955: BWI76_RS04955 - hypoxanthine phosphoribosyltransferase, at 1,041,584 to 1,042,120
_RS04955
BWI76_RS04960: BWI76_RS04960 - carbonate dehydratase, at 1,042,171 to 1,042,833
_RS04960
BWI76_RS04965: BWI76_RS04965 - ABC transporter ATP-binding protein, at 1,042,941 to 1,043,867
_RS04965
Position (kb) 1041
1042
1043 Strain fitness (log2 ratio) -2
-1
0
1 at 1040.694 kb on - strand, within BWI76_RS04950 at 1040.852 kb on - strand, within BWI76_RS04950 at 1040.873 kb on - strand, within BWI76_RS04950 at 1041.073 kb on - strand, within BWI76_RS04950 at 1041.210 kb on - strand at 1041.258 kb on - strand at 1041.498 kb on + strand at 1041.749 kb on + strand, within BWI76_RS04955 at 1041.801 kb on - strand, within BWI76_RS04955 at 1042.160 kb on - strand at 1042.160 kb on - strand at 1042.279 kb on + strand, within BWI76_RS04960 at 1042.303 kb on - strand at 1042.491 kb on + strand, within BWI76_RS04960 at 1042.585 kb on + strand, within BWI76_RS04960 at 1042.624 kb on - strand, within BWI76_RS04960 at 1042.785 kb on + strand at 1043.048 kb on + strand, within BWI76_RS04965
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 1,040,694 - BWI76_RS04950 0.71 -1.3 1,040,852 - BWI76_RS04950 0.78 -1.3 1,040,873 - BWI76_RS04950 0.79 -1.8 1,041,073 - BWI76_RS04950 0.87 +0.9 1,041,210 - -1.8 1,041,258 - +0.3 1,041,498 + +0.3 1,041,749 + BWI76_RS04955 0.31 -0.6 1,041,801 - BWI76_RS04955 0.40 +1.1 1,042,160 - +0.6 1,042,160 - +0.6 1,042,279 + BWI76_RS04960 0.16 -0.2 1,042,303 - +0.7 1,042,491 + BWI76_RS04960 0.48 +0.5 1,042,585 + BWI76_RS04960 0.62 +0.6 1,042,624 - BWI76_RS04960 0.68 +0.6 1,042,785 + +0.5 1,043,048 + BWI76_RS04965 0.12 +0.4
Or see this region's nucleotide sequence