Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04175 and BWI76_RS04180 overlap by 60 nucleotides BWI76_RS04180 and BWI76_RS04185 are separated by 125 nucleotides BWI76_RS04185 and BWI76_RS04190 are separated by 117 nucleotides BWI76_RS04190 and BWI76_RS04195 overlap by 20 nucleotides
BWI76_RS04175: BWI76_RS04175 - energy-dependent translational throttle protein EttA, at 859,388 to 861,055
_RS04175
BWI76_RS04180: BWI76_RS04180 - hypothetical protein, at 860,996 to 861,203
_RS04180
BWI76_RS04185: BWI76_RS04185 - murein transglycosylase, at 861,329 to 863,266
_RS04185
BWI76_RS04190: BWI76_RS04190 - Trp operon repressor, at 863,384 to 863,716
_RS04190
BWI76_RS04195: BWI76_RS04195 - non-canonical purine NTP phosphatase, at 863,697 to 864,218
_RS04195
Position (kb) 861
862
863
864 Strain fitness (log2 ratio) -2
-1
0
1
2 at 861.021 kb on - strand at 861.062 kb on - strand, within BWI76_RS04180 at 861.082 kb on + strand, within BWI76_RS04180 at 861.118 kb on + strand, within BWI76_RS04180 at 861.126 kb on - strand, within BWI76_RS04180 at 861.131 kb on - strand, within BWI76_RS04180 at 861.133 kb on + strand, within BWI76_RS04180 at 861.176 kb on + strand, within BWI76_RS04180 at 861.293 kb on + strand at 861.530 kb on - strand, within BWI76_RS04185 at 861.687 kb on + strand, within BWI76_RS04185 at 861.768 kb on - strand, within BWI76_RS04185 at 861.778 kb on - strand, within BWI76_RS04185 at 861.835 kb on + strand, within BWI76_RS04185 at 861.897 kb on - strand, within BWI76_RS04185 at 862.016 kb on + strand, within BWI76_RS04185 at 862.085 kb on + strand, within BWI76_RS04185 at 862.128 kb on - strand, within BWI76_RS04185 at 862.161 kb on + strand, within BWI76_RS04185 at 862.182 kb on - strand, within BWI76_RS04185 at 862.458 kb on + strand, within BWI76_RS04185 at 862.495 kb on + strand, within BWI76_RS04185 at 862.695 kb on - strand, within BWI76_RS04185 at 863.062 kb on - strand, within BWI76_RS04185 at 863.231 kb on + strand at 863.241 kb on - strand at 863.283 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 861,021 - +0.7 861,062 - BWI76_RS04180 0.32 -0.9 861,082 + BWI76_RS04180 0.41 +1.6 861,118 + BWI76_RS04180 0.59 +0.1 861,126 - BWI76_RS04180 0.62 +1.0 861,131 - BWI76_RS04180 0.65 +1.8 861,133 + BWI76_RS04180 0.66 -1.1 861,176 + BWI76_RS04180 0.87 +0.8 861,293 + +1.2 861,530 - BWI76_RS04185 0.10 -1.9 861,687 + BWI76_RS04185 0.18 +0.1 861,768 - BWI76_RS04185 0.23 -1.0 861,778 - BWI76_RS04185 0.23 -0.4 861,835 + BWI76_RS04185 0.26 -0.7 861,897 - BWI76_RS04185 0.29 +0.4 862,016 + BWI76_RS04185 0.35 -2.0 862,085 + BWI76_RS04185 0.39 +0.3 862,128 - BWI76_RS04185 0.41 -0.6 862,161 + BWI76_RS04185 0.43 -0.1 862,182 - BWI76_RS04185 0.44 -0.1 862,458 + BWI76_RS04185 0.58 -1.7 862,495 + BWI76_RS04185 0.60 -0.3 862,695 - BWI76_RS04185 0.70 +0.2 863,062 - BWI76_RS04185 0.89 -1.1 863,231 + +0.4 863,241 - -0.4 863,283 - +0.5
Or see this region's nucleotide sequence