Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03870 and BWI76_RS03875 overlap by 4 nucleotides BWI76_RS03875 and BWI76_RS03880 are separated by 284 nucleotides BWI76_RS03880 and BWI76_RS03885 are separated by 118 nucleotides
BWI76_RS03870: BWI76_RS03870 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 800,637 to 801,401
_RS03870
BWI76_RS03875: BWI76_RS03875 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 801,398 to 802,030
_RS03875
BWI76_RS03880: BWI76_RS03880 - homoprotocatechuate degradation operon regulator, HpaR, at 802,315 to 802,752
_RS03880
BWI76_RS03885: BWI76_RS03885 - hypothetical protein, at 802,871 to 804,085
_RS03885
Position (kb) 802
803 Strain fitness (log2 ratio) -2
-1
0
1
2 at 801.351 kb on + strand at 801.494 kb on + strand, within BWI76_RS03875 at 801.651 kb on + strand, within BWI76_RS03875 at 801.950 kb on - strand, within BWI76_RS03875 at 801.957 kb on + strand, within BWI76_RS03875 at 802.313 kb on - strand at 802.514 kb on + strand, within BWI76_RS03880 at 802.532 kb on + strand, within BWI76_RS03880 at 802.540 kb on - strand, within BWI76_RS03880 at 802.741 kb on + strand at 802.891 kb on - strand at 802.899 kb on + strand at 802.900 kb on + strand at 803.270 kb on + strand, within BWI76_RS03885 at 803.376 kb on - strand, within BWI76_RS03885 at 803.376 kb on - strand, within BWI76_RS03885 at 803.398 kb on + strand, within BWI76_RS03885 at 803.502 kb on + strand, within BWI76_RS03885 at 803.510 kb on - strand, within BWI76_RS03885 at 803.520 kb on - strand, within BWI76_RS03885 at 803.535 kb on - strand, within BWI76_RS03885 at 803.541 kb on - strand, within BWI76_RS03885
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 801,351 + +0.7 801,494 + BWI76_RS03875 0.15 +0.5 801,651 + BWI76_RS03875 0.40 +0.1 801,950 - BWI76_RS03875 0.87 -0.4 801,957 + BWI76_RS03875 0.88 -0.2 802,313 - +1.6 802,514 + BWI76_RS03880 0.45 +1.7 802,532 + BWI76_RS03880 0.50 +0.8 802,540 - BWI76_RS03880 0.51 +2.7 802,741 + +1.5 802,891 - +1.3 802,899 + -0.1 802,900 + -1.4 803,270 + BWI76_RS03885 0.33 +0.1 803,376 - BWI76_RS03885 0.42 +0.4 803,376 - BWI76_RS03885 0.42 -0.4 803,398 + BWI76_RS03885 0.43 -0.1 803,502 + BWI76_RS03885 0.52 +0.5 803,510 - BWI76_RS03885 0.53 +0.4 803,520 - BWI76_RS03885 0.53 -0.8 803,535 - BWI76_RS03885 0.55 -2.7 803,541 - BWI76_RS03885 0.55 +0.5
Or see this region's nucleotide sequence