Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03850 and BWI76_RS03855 are separated by 136 nucleotides BWI76_RS03855 and BWI76_RS03860 are separated by 12 nucleotides BWI76_RS03860 and BWI76_RS03865 are separated by 1 nucleotides
BWI76_RS03850: BWI76_RS03850 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 796,982 to 797,785
_RS03850
BWI76_RS03855: BWI76_RS03855 - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 797,922 to 798,302
_RS03855
BWI76_RS03860: BWI76_RS03860 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 798,315 to 799,172
_RS03860
BWI76_RS03865: BWI76_RS03865 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 799,174 to 800,640
_RS03865
Position (kb) 798
799
800 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 797.440 kb on - strand, within BWI76_RS03850 at 797.544 kb on + strand, within BWI76_RS03850 at 797.552 kb on - strand at 797.769 kb on - strand at 797.849 kb on - strand at 798.231 kb on - strand, within BWI76_RS03855 at 798.313 kb on + strand at 798.343 kb on + strand at 798.496 kb on + strand, within BWI76_RS03860 at 798.504 kb on - strand, within BWI76_RS03860 at 798.732 kb on - strand, within BWI76_RS03860 at 798.886 kb on + strand, within BWI76_RS03860 at 798.907 kb on - strand, within BWI76_RS03860 at 798.996 kb on - strand, within BWI76_RS03860 at 798.996 kb on - strand, within BWI76_RS03860 at 799.214 kb on + strand at 799.250 kb on - strand at 799.274 kb on + strand at 799.282 kb on - strand at 799.422 kb on - strand, within BWI76_RS03865 at 799.445 kb on - strand, within BWI76_RS03865 at 799.519 kb on + strand, within BWI76_RS03865 at 799.832 kb on + strand, within BWI76_RS03865 at 799.840 kb on - strand, within BWI76_RS03865 at 799.850 kb on - strand, within BWI76_RS03865 at 800.010 kb on + strand, within BWI76_RS03865 at 800.136 kb on - strand, within BWI76_RS03865
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 797,440 - BWI76_RS03850 0.57 -0.9 797,544 + BWI76_RS03850 0.70 +0.0 797,552 - +1.1 797,769 - +0.1 797,849 - -0.0 798,231 - BWI76_RS03855 0.81 -0.6 798,313 + +0.5 798,343 + +0.2 798,496 + BWI76_RS03860 0.21 -0.8 798,504 - BWI76_RS03860 0.22 -0.6 798,732 - BWI76_RS03860 0.49 +0.3 798,886 + BWI76_RS03860 0.67 -0.4 798,907 - BWI76_RS03860 0.69 +0.3 798,996 - BWI76_RS03860 0.79 +1.1 798,996 - BWI76_RS03860 0.79 -0.7 799,214 + -1.9 799,250 - +1.5 799,274 + +0.3 799,282 - +0.8 799,422 - BWI76_RS03865 0.17 -1.3 799,445 - BWI76_RS03865 0.18 +1.5 799,519 + BWI76_RS03865 0.24 +0.0 799,832 + BWI76_RS03865 0.45 +0.4 799,840 - BWI76_RS03865 0.45 +0.5 799,850 - BWI76_RS03865 0.46 -3.3 800,010 + BWI76_RS03865 0.57 +0.4 800,136 - BWI76_RS03865 0.66 +0.4
Or see this region's nucleotide sequence