Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03610 and BWI76_RS03615 overlap by 4 nucleotides BWI76_RS03615 and BWI76_RS03620 are separated by 17 nucleotides BWI76_RS03620 and BWI76_RS03625 are separated by 147 nucleotides
BWI76_RS03610: BWI76_RS03610 - hypothetical protein, at 747,244 to 747,762
_RS03610
BWI76_RS03615: BWI76_RS03615 - putative transcriptional regulatory protein, at 747,759 to 748,559
_RS03615
BWI76_RS03620: BWI76_RS03620 - TetR family transcriptional regulator, at 748,577 to 749,161
_RS03620
BWI76_RS03625: BWI76_RS03625 - NAD(P)H quinone oxidoreductase, at 749,309 to 750,091
_RS03625
Position (kb) 748
749
750 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 747.652 kb on + strand, within BWI76_RS03610 at 747.722 kb on + strand at 747.809 kb on + strand at 747.856 kb on - strand, within BWI76_RS03615 at 748.031 kb on + strand, within BWI76_RS03615 at 748.040 kb on + strand, within BWI76_RS03615 at 748.255 kb on + strand, within BWI76_RS03615 at 748.265 kb on + strand, within BWI76_RS03615 at 748.265 kb on + strand, within BWI76_RS03615 at 748.265 kb on + strand, within BWI76_RS03615 at 748.369 kb on + strand, within BWI76_RS03615 at 748.574 kb on + strand at 748.728 kb on + strand, within BWI76_RS03620 at 748.814 kb on - strand, within BWI76_RS03620 at 748.823 kb on + strand, within BWI76_RS03620 at 748.823 kb on + strand, within BWI76_RS03620 at 748.859 kb on + strand, within BWI76_RS03620 at 748.867 kb on - strand, within BWI76_RS03620 at 749.145 kb on - strand at 749.219 kb on - strand at 749.320 kb on - strand at 749.376 kb on - strand at 749.381 kb on + strand at 749.483 kb on - strand, within BWI76_RS03625 at 749.530 kb on + strand, within BWI76_RS03625 at 749.538 kb on - strand, within BWI76_RS03625 at 749.568 kb on + strand, within BWI76_RS03625 at 749.610 kb on + strand at 749.739 kb on + strand, within BWI76_RS03625 at 749.739 kb on + strand, within BWI76_RS03625 at 749.754 kb on + strand, within BWI76_RS03625 at 749.757 kb on - strand, within BWI76_RS03625 at 749.970 kb on + strand, within BWI76_RS03625 at 749.978 kb on - strand, within BWI76_RS03625 at 749.984 kb on + strand, within BWI76_RS03625 at 750.123 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 747,652 + BWI76_RS03610 0.79 -0.5 747,722 + +2.3 747,809 + +1.5 747,856 - BWI76_RS03615 0.12 -0.2 748,031 + BWI76_RS03615 0.34 +0.6 748,040 + BWI76_RS03615 0.35 +0.3 748,255 + BWI76_RS03615 0.62 +3.4 748,265 + BWI76_RS03615 0.63 -0.8 748,265 + BWI76_RS03615 0.63 -0.4 748,265 + BWI76_RS03615 0.63 +1.5 748,369 + BWI76_RS03615 0.76 -0.1 748,574 + +1.9 748,728 + BWI76_RS03620 0.26 +3.4 748,814 - BWI76_RS03620 0.41 +0.5 748,823 + BWI76_RS03620 0.42 +1.0 748,823 + BWI76_RS03620 0.42 +0.3 748,859 + BWI76_RS03620 0.48 +0.9 748,867 - BWI76_RS03620 0.50 +0.8 749,145 - -1.2 749,219 - +1.8 749,320 - +1.1 749,376 - +3.1 749,381 + +1.1 749,483 - BWI76_RS03625 0.22 +3.1 749,530 + BWI76_RS03625 0.28 +0.3 749,538 - BWI76_RS03625 0.29 +0.2 749,568 + BWI76_RS03625 0.33 +0.9 749,610 + +1.1 749,739 + BWI76_RS03625 0.55 -1.9 749,739 + BWI76_RS03625 0.55 +0.7 749,754 + BWI76_RS03625 0.57 -0.9 749,757 - BWI76_RS03625 0.57 -2.6 749,970 + BWI76_RS03625 0.84 +0.3 749,978 - BWI76_RS03625 0.85 +0.6 749,984 + BWI76_RS03625 0.86 +1.1 750,123 + +0.7
Or see this region's nucleotide sequence