Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02850 and BWI76_RS02855 are separated by 36 nucleotides BWI76_RS02855 and BWI76_RS02860 are separated by 419 nucleotides BWI76_RS02860 and BWI76_RS02865 are separated by 11 nucleotides
BWI76_RS02850: BWI76_RS02850 - metalloprotease PmbA, at 583,827 to 585,179
_RS02850
BWI76_RS02855: BWI76_RS02855 - cytochrome B562, at 585,216 to 585,602
_RS02855
BWI76_RS02860: BWI76_RS02860 - glycine dehydrogenase, at 586,022 to 586,360
_RS02860
BWI76_RS02865: BWI76_RS02865 - hypothetical protein, at 586,372 to 586,734
_RS02865
Position (kb) 585
586 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 584.441 kb on + strand, within BWI76_RS02850 at 584.449 kb on - strand, within BWI76_RS02850 at 584.509 kb on + strand, within BWI76_RS02850 at 584.516 kb on - strand, within BWI76_RS02850 at 584.800 kb on + strand, within BWI76_RS02850 at 584.808 kb on - strand, within BWI76_RS02850 at 585.139 kb on + strand at 585.305 kb on - strand, within BWI76_RS02855 at 585.328 kb on - strand, within BWI76_RS02855 at 585.480 kb on - strand, within BWI76_RS02855 at 585.568 kb on - strand at 585.981 kb on + strand at 585.981 kb on + strand at 586.011 kb on - strand at 586.055 kb on - strand at 586.112 kb on + strand, within BWI76_RS02860 at 586.296 kb on - strand, within BWI76_RS02860 at 586.357 kb on + strand at 586.410 kb on - strand, within BWI76_RS02865 at 586.553 kb on + strand, within BWI76_RS02865 at 586.553 kb on + strand, within BWI76_RS02865 at 586.560 kb on + strand, within BWI76_RS02865 at 586.566 kb on - strand, within BWI76_RS02865
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 584,441 + BWI76_RS02850 0.45 +1.2 584,449 - BWI76_RS02850 0.46 -0.0 584,509 + BWI76_RS02850 0.50 -2.0 584,516 - BWI76_RS02850 0.51 +0.6 584,800 + BWI76_RS02850 0.72 +0.6 584,808 - BWI76_RS02850 0.73 -0.3 585,139 + +1.2 585,305 - BWI76_RS02855 0.23 -0.8 585,328 - BWI76_RS02855 0.29 -0.9 585,480 - BWI76_RS02855 0.68 +1.2 585,568 - -0.0 585,981 + +0.5 585,981 + +0.9 586,011 - +0.3 586,055 - +2.0 586,112 + BWI76_RS02860 0.27 -1.1 586,296 - BWI76_RS02860 0.81 +0.7 586,357 + -3.7 586,410 - BWI76_RS02865 0.10 +2.5 586,553 + BWI76_RS02865 0.50 -0.3 586,553 + BWI76_RS02865 0.50 -0.6 586,560 + BWI76_RS02865 0.52 -0.3 586,566 - BWI76_RS02865 0.53 +1.9
Or see this region's nucleotide sequence