Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02145 and BWI76_RS02150 overlap by 17 nucleotides BWI76_RS02150 and BWI76_RS02155 are separated by 10 nucleotides BWI76_RS02155 and BWI76_RS02160 overlap by 22 nucleotides
BWI76_RS02145: BWI76_RS02145 - allose kinase, at 446,104 to 447,030
_RS02145
BWI76_RS02150: BWI76_RS02150 - allulose-6-phosphate 3-epimerase, at 447,014 to 447,709
_RS02150
BWI76_RS02155: BWI76_RS02155 - D-allose transporter subunit, at 447,720 to 448,700
_RS02155
BWI76_RS02160: BWI76_RS02160 - D-allose transporter ATP-binding protein, at 448,679 to 450,211
_RS02160
Position (kb) 447
448
449 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 446.725 kb on - strand, within BWI76_RS02145 at 446.725 kb on - strand, within BWI76_RS02145 at 446.725 kb on - strand, within BWI76_RS02145 at 446.730 kb on + strand, within BWI76_RS02145 at 446.913 kb on + strand, within BWI76_RS02145 at 446.916 kb on - strand, within BWI76_RS02145 at 446.943 kb on + strand at 446.985 kb on - strand at 446.985 kb on - strand at 446.998 kb on - strand at 447.029 kb on - strand at 447.123 kb on - strand, within BWI76_RS02150 at 447.481 kb on - strand, within BWI76_RS02150 at 447.487 kb on + strand, within BWI76_RS02150 at 447.492 kb on + strand, within BWI76_RS02150 at 447.500 kb on - strand, within BWI76_RS02150 at 447.500 kb on - strand, within BWI76_RS02150 at 447.581 kb on + strand, within BWI76_RS02150 at 447.676 kb on + strand at 447.676 kb on + strand at 447.676 kb on + strand at 447.684 kb on - strand at 447.686 kb on - strand at 447.686 kb on - strand at 447.694 kb on - strand at 447.694 kb on - strand at 447.792 kb on + strand at 448.074 kb on - strand, within BWI76_RS02155 at 448.221 kb on + strand, within BWI76_RS02155 at 448.229 kb on - strand, within BWI76_RS02155 at 448.412 kb on + strand, within BWI76_RS02155 at 448.412 kb on + strand, within BWI76_RS02155 at 448.564 kb on - strand, within BWI76_RS02155 at 448.638 kb on - strand at 448.638 kb on - strand at 448.766 kb on + strand at 448.774 kb on - strand at 448.774 kb on - strand at 448.799 kb on - strand at 448.853 kb on + strand, within BWI76_RS02160 at 448.888 kb on + strand, within BWI76_RS02160 at 448.924 kb on + strand, within BWI76_RS02160 at 448.997 kb on - strand, within BWI76_RS02160 at 449.113 kb on - strand, within BWI76_RS02160 at 449.124 kb on + strand, within BWI76_RS02160 at 449.129 kb on + strand, within BWI76_RS02160 at 449.137 kb on - strand, within BWI76_RS02160 at 449.137 kb on - strand, within BWI76_RS02160 at 449.218 kb on - strand, within BWI76_RS02160 at 449.308 kb on + strand, within BWI76_RS02160 at 449.495 kb on - strand, within BWI76_RS02160 at 449.520 kb on + strand, within BWI76_RS02160 at 449.593 kb on - strand, within BWI76_RS02160
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 446,725 - BWI76_RS02145 0.67 +1.3 446,725 - BWI76_RS02145 0.67 -0.8 446,725 - BWI76_RS02145 0.67 -0.8 446,730 + BWI76_RS02145 0.68 +1.1 446,913 + BWI76_RS02145 0.87 +0.9 446,916 - BWI76_RS02145 0.88 +0.0 446,943 + +0.0 446,985 - +1.1 446,985 - -0.8 446,998 - +1.3 447,029 - +1.1 447,123 - BWI76_RS02150 0.16 +0.3 447,481 - BWI76_RS02150 0.67 +0.4 447,487 + BWI76_RS02150 0.68 -2.4 447,492 + BWI76_RS02150 0.69 -0.1 447,500 - BWI76_RS02150 0.70 +1.9 447,500 - BWI76_RS02150 0.70 -0.6 447,581 + BWI76_RS02150 0.81 +0.3 447,676 + -1.8 447,676 + -2.5 447,676 + +0.8 447,684 - +1.2 447,686 - +0.4 447,686 - +0.1 447,694 - +0.1 447,694 - -0.1 447,792 + +0.3 448,074 - BWI76_RS02155 0.36 -0.1 448,221 + BWI76_RS02155 0.51 -0.3 448,229 - BWI76_RS02155 0.52 -1.4 448,412 + BWI76_RS02155 0.71 -1.3 448,412 + BWI76_RS02155 0.71 +0.8 448,564 - BWI76_RS02155 0.86 -1.6 448,638 - +0.9 448,638 - -0.4 448,766 + +0.4 448,774 - -0.4 448,774 - +1.0 448,799 - -1.8 448,853 + BWI76_RS02160 0.11 -0.0 448,888 + BWI76_RS02160 0.14 -0.3 448,924 + BWI76_RS02160 0.16 +0.7 448,997 - BWI76_RS02160 0.21 +1.2 449,113 - BWI76_RS02160 0.28 -0.6 449,124 + BWI76_RS02160 0.29 -0.4 449,129 + BWI76_RS02160 0.29 +0.4 449,137 - BWI76_RS02160 0.30 +0.7 449,137 - BWI76_RS02160 0.30 +0.6 449,218 - BWI76_RS02160 0.35 +0.1 449,308 + BWI76_RS02160 0.41 +0.2 449,495 - BWI76_RS02160 0.53 -2.8 449,520 + BWI76_RS02160 0.55 -0.3 449,593 - BWI76_RS02160 0.60 -1.4
Or see this region's nucleotide sequence