Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_785

Experiment: 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_784 and Pf6N2E2_785 are separated by 37 nucleotidesPf6N2E2_785 and Pf6N2E2_786 are separated by 109 nucleotidesPf6N2E2_786 and Pf6N2E2_787 are separated by 253 nucleotides Pf6N2E2_784 - Outer membrane usher protein FIMD, at 897,590 to 900,085 _784 Pf6N2E2_785 - P pilus assembly protein, chaperone PapD, at 900,123 to 900,884 _785 Pf6N2E2_786 - Type-1 fimbrial protein, A chain precursor, at 900,994 to 901,542 _786 Pf6N2E2_787 - YD repeat protein, at 901,796 to 906,883 _787 Position (kb) 900 901Strain fitness (log2 ratio) -2 -1 0 1 2at 899.136 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.154 kb on + strand, within Pf6N2E2_784at 899.155 kb on - strand, within Pf6N2E2_784at 899.155 kb on - strand, within Pf6N2E2_784at 899.155 kb on - strand, within Pf6N2E2_784at 899.155 kb on - strand, within Pf6N2E2_784at 899.164 kb on - strandat 899.379 kb on + strand, within Pf6N2E2_784at 899.379 kb on + strand, within Pf6N2E2_784at 899.379 kb on + strand, within Pf6N2E2_784at 899.379 kb on + strand, within Pf6N2E2_784at 899.379 kb on + strand, within Pf6N2E2_784at 899.379 kb on + strand, within Pf6N2E2_784at 899.379 kb on + strand, within Pf6N2E2_784at 899.380 kb on - strand, within Pf6N2E2_784at 899.383 kb on + strand, within Pf6N2E2_784at 899.383 kb on + strand, within Pf6N2E2_784at 899.383 kb on + strand, within Pf6N2E2_784at 899.383 kb on + strand, within Pf6N2E2_784at 899.383 kb on + strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.384 kb on - strand, within Pf6N2E2_784at 899.385 kb on + strand, within Pf6N2E2_784at 899.385 kb on + strand, within Pf6N2E2_784at 899.481 kb on + strand, within Pf6N2E2_784at 899.544 kb on + strand, within Pf6N2E2_784at 899.544 kb on + strand, within Pf6N2E2_784at 899.544 kb on + strand, within Pf6N2E2_784at 899.544 kb on - strand, within Pf6N2E2_784at 899.671 kb on + strand, within Pf6N2E2_784at 899.688 kb on - strand, within Pf6N2E2_784at 899.750 kb on + strand, within Pf6N2E2_784at 899.750 kb on + strand, within Pf6N2E2_784at 899.750 kb on + strand, within Pf6N2E2_784at 899.751 kb on - strand, within Pf6N2E2_784at 899.751 kb on - strand, within Pf6N2E2_784at 899.751 kb on - strand, within Pf6N2E2_784at 900.098 kb on + strandat 900.278 kb on + strand, within Pf6N2E2_785at 900.279 kb on - strand, within Pf6N2E2_785at 900.352 kb on + strand, within Pf6N2E2_785at 900.352 kb on + strand, within Pf6N2E2_785at 900.352 kb on + strand, within Pf6N2E2_785at 900.479 kb on + strand, within Pf6N2E2_785at 900.535 kb on + strand, within Pf6N2E2_785at 900.535 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.699 kb on + strand, within Pf6N2E2_785at 900.700 kb on - strand, within Pf6N2E2_785at 900.700 kb on - strand, within Pf6N2E2_785at 900.701 kb on + strand, within Pf6N2E2_785at 900.892 kb on + strandat 900.958 kb on + strandat 901.116 kb on - strand, within Pf6N2E2_786at 901.255 kb on - strand, within Pf6N2E2_786at 901.377 kb on - strand, within Pf6N2E2_786at 901.377 kb on - strand, within Pf6N2E2_786at 901.377 kb on - strand, within Pf6N2E2_786at 901.377 kb on - strand, within Pf6N2E2_786at 901.377 kb on - strand, within Pf6N2E2_786at 901.638 kb on + strandat 901.644 kb on + strandat 901.644 kb on + strandat 901.877 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
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899,136 + Pf6N2E2_784 0.62 +1.4
899,154 + Pf6N2E2_784 0.63 -0.4
899,154 + Pf6N2E2_784 0.63 -0.1
899,154 + Pf6N2E2_784 0.63 +0.4
899,154 + Pf6N2E2_784 0.63 +0.8
899,154 + Pf6N2E2_784 0.63 +1.1
899,154 + Pf6N2E2_784 0.63 +0.4
899,154 + Pf6N2E2_784 0.63 +0.9
899,155 - Pf6N2E2_784 0.63 -0.3
899,155 - Pf6N2E2_784 0.63 +0.0
899,155 - Pf6N2E2_784 0.63 -0.6
899,155 - Pf6N2E2_784 0.63 +0.1
899,164 - +0.4
899,379 + Pf6N2E2_784 0.72 +2.6
899,379 + Pf6N2E2_784 0.72 +1.8
899,379 + Pf6N2E2_784 0.72 +0.1
899,379 + Pf6N2E2_784 0.72 -0.0
899,379 + Pf6N2E2_784 0.72 +0.6
899,379 + Pf6N2E2_784 0.72 -0.6
899,379 + Pf6N2E2_784 0.72 -0.8
899,380 - Pf6N2E2_784 0.72 -1.0
899,383 + Pf6N2E2_784 0.72 -1.6
899,383 + Pf6N2E2_784 0.72 -0.6
899,383 + Pf6N2E2_784 0.72 +0.5
899,383 + Pf6N2E2_784 0.72 -1.6
899,383 + Pf6N2E2_784 0.72 -1.0
899,384 - Pf6N2E2_784 0.72 +1.0
899,384 - Pf6N2E2_784 0.72 -0.2
899,384 - Pf6N2E2_784 0.72 +1.2
899,384 - Pf6N2E2_784 0.72 -0.6
899,384 - Pf6N2E2_784 0.72 +0.4
899,384 - Pf6N2E2_784 0.72 -0.1
899,384 - Pf6N2E2_784 0.72 -0.2
899,384 - Pf6N2E2_784 0.72 -0.6
899,385 + Pf6N2E2_784 0.72 -0.2
899,385 + Pf6N2E2_784 0.72 +0.2
899,481 + Pf6N2E2_784 0.76 -0.3
899,544 + Pf6N2E2_784 0.78 +1.0
899,544 + Pf6N2E2_784 0.78 +1.0
899,544 + Pf6N2E2_784 0.78 +0.6
899,544 - Pf6N2E2_784 0.78 +0.4
899,671 + Pf6N2E2_784 0.83 +0.4
899,688 - Pf6N2E2_784 0.84 -1.1
899,750 + Pf6N2E2_784 0.87 +0.4
899,750 + Pf6N2E2_784 0.87 +0.0
899,750 + Pf6N2E2_784 0.87 -1.8
899,751 - Pf6N2E2_784 0.87 -0.6
899,751 - Pf6N2E2_784 0.87 +0.4
899,751 - Pf6N2E2_784 0.87 +0.4
900,098 + +0.6
900,278 + Pf6N2E2_785 0.20 +0.7
900,279 - Pf6N2E2_785 0.20 -0.6
900,352 + Pf6N2E2_785 0.30 +0.3
900,352 + Pf6N2E2_785 0.30 +0.7
900,352 + Pf6N2E2_785 0.30 +1.2
900,479 + Pf6N2E2_785 0.47 +0.6
900,535 + Pf6N2E2_785 0.54 +2.0
900,535 + Pf6N2E2_785 0.54 +1.9
900,699 + Pf6N2E2_785 0.76 +1.0
900,699 + Pf6N2E2_785 0.76 +0.1
900,699 + Pf6N2E2_785 0.76 -0.6
900,699 + Pf6N2E2_785 0.76 -0.6
900,699 + Pf6N2E2_785 0.76 -0.3
900,700 - Pf6N2E2_785 0.76 +1.4
900,700 - Pf6N2E2_785 0.76 +0.9
900,701 + Pf6N2E2_785 0.76 +2.2
900,892 + +0.8
900,958 + +1.2
901,116 - Pf6N2E2_786 0.22 +1.8
901,255 - Pf6N2E2_786 0.48 +0.2
901,377 - Pf6N2E2_786 0.70 +0.7
901,377 - Pf6N2E2_786 0.70 -0.1
901,377 - Pf6N2E2_786 0.70 -0.6
901,377 - Pf6N2E2_786 0.70 +0.1
901,377 - Pf6N2E2_786 0.70 -0.7
901,638 + -2.1
901,644 + +1.8
901,644 + -1.0
901,877 + -0.6

Or see this region's nucleotide sequence