Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1716

Experiment: 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1714 and Pf6N2E2_1715 are separated by 1 nucleotidesPf6N2E2_1715 and Pf6N2E2_1716 are separated by 8 nucleotidesPf6N2E2_1716 and Pf6N2E2_1717 are separated by 651 nucleotides Pf6N2E2_1714 - FIG01218476: hypothetical protein, at 2,022,370 to 2,023,053 _1714 Pf6N2E2_1715 - hypothetical protein, at 2,023,055 to 2,023,600 _1715 Pf6N2E2_1716 - Phenylacetate-coenzyme A ligase (EC 6.2.1.30), at 2,023,609 to 2,024,961 _1716 Pf6N2E2_1717 - Anthranilate synthase, aminase component (EC 4.1.3.27), at 2,025,613 to 2,027,082 _1717 Position (kb) 2023 2024 2025Strain fitness (log2 ratio) -2 -1 0 1at 2022.638 kb on + strand, within Pf6N2E2_1714at 2022.662 kb on + strand, within Pf6N2E2_1714at 2022.912 kb on - strand, within Pf6N2E2_1714at 2022.912 kb on - strand, within Pf6N2E2_1714at 2022.917 kb on + strand, within Pf6N2E2_1714at 2022.942 kb on - strand, within Pf6N2E2_1714at 2023.102 kb on - strandat 2023.182 kb on + strand, within Pf6N2E2_1715at 2023.183 kb on - strand, within Pf6N2E2_1715at 2023.446 kb on + strand, within Pf6N2E2_1715at 2023.447 kb on - strand, within Pf6N2E2_1715at 2023.527 kb on + strand, within Pf6N2E2_1715at 2024.129 kb on + strand, within Pf6N2E2_1716at 2024.129 kb on + strand, within Pf6N2E2_1716at 2024.130 kb on - strand, within Pf6N2E2_1716at 2024.130 kb on - strand, within Pf6N2E2_1716at 2024.130 kb on - strand, within Pf6N2E2_1716at 2024.130 kb on - strand, within Pf6N2E2_1716at 2024.130 kb on - strand, within Pf6N2E2_1716at 2024.130 kb on - strand, within Pf6N2E2_1716at 2024.237 kb on + strand, within Pf6N2E2_1716at 2024.517 kb on + strand, within Pf6N2E2_1716at 2024.610 kb on - strand, within Pf6N2E2_1716at 2024.672 kb on + strand, within Pf6N2E2_1716at 2024.907 kb on - strandat 2025.025 kb on - strandat 2025.027 kb on - strandat 2025.093 kb on - strandat 2025.097 kb on - strandat 2025.109 kb on - strandat 2025.347 kb on + strandat 2025.606 kb on - strandat 2025.683 kb on + strandat 2025.685 kb on + strandat 2025.914 kb on + strand, within Pf6N2E2_1717

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
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2,022,638 + Pf6N2E2_1714 0.39 -0.6
2,022,662 + Pf6N2E2_1714 0.43 +0.1
2,022,912 - Pf6N2E2_1714 0.79 -0.9
2,022,912 - Pf6N2E2_1714 0.79 +0.9
2,022,917 + Pf6N2E2_1714 0.80 +0.8
2,022,942 - Pf6N2E2_1714 0.84 +1.7
2,023,102 - -1.2
2,023,182 + Pf6N2E2_1715 0.23 +0.3
2,023,183 - Pf6N2E2_1715 0.23 -0.6
2,023,446 + Pf6N2E2_1715 0.72 +0.6
2,023,447 - Pf6N2E2_1715 0.72 +0.4
2,023,527 + Pf6N2E2_1715 0.86 +0.4
2,024,129 + Pf6N2E2_1716 0.38 -0.9
2,024,129 + Pf6N2E2_1716 0.38 -0.4
2,024,130 - Pf6N2E2_1716 0.39 -0.6
2,024,130 - Pf6N2E2_1716 0.39 -0.6
2,024,130 - Pf6N2E2_1716 0.39 -0.8
2,024,130 - Pf6N2E2_1716 0.39 +0.0
2,024,130 - Pf6N2E2_1716 0.39 -0.4
2,024,130 - Pf6N2E2_1716 0.39 +0.7
2,024,237 + Pf6N2E2_1716 0.46 -1.6
2,024,517 + Pf6N2E2_1716 0.67 -0.1
2,024,610 - Pf6N2E2_1716 0.74 -1.3
2,024,672 + Pf6N2E2_1716 0.79 +0.0
2,024,907 - -0.6
2,025,025 - -1.1
2,025,027 - +0.1
2,025,093 - -0.1
2,025,097 - -1.6
2,025,109 - +0.2
2,025,347 + -1.3
2,025,606 - +1.0
2,025,683 + -0.6
2,025,685 + +1.4
2,025,914 + Pf6N2E2_1717 0.20 -2.1

Or see this region's nucleotide sequence