Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03645 and BWI76_RS03650 are separated by 451 nucleotides BWI76_RS03650 and BWI76_RS03655 overlap by 2 nucleotides BWI76_RS03655 and BWI76_RS03660 overlap by 29 nucleotides
BWI76_RS03645: BWI76_RS03645 - hypothetical protein, at 753,023 to 753,934
_RS03645
BWI76_RS03650: BWI76_RS03650 - endoribonuclease SymE, at 754,386 to 754,721
_RS03650
BWI76_RS03655: BWI76_RS03655 - hypothetical protein, at 754,720 to 754,920
_RS03655
BWI76_RS03660: BWI76_RS03660 - hypothetical protein, at 754,892 to 756,757
_RS03660
Position (kb)
754
755 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 753.580 kb on + strand, within BWI76_RS03645 at 753.673 kb on - strand, within BWI76_RS03645 at 753.684 kb on - strand, within BWI76_RS03645 at 753.730 kb on + strand, within BWI76_RS03645 at 753.744 kb on + strand, within BWI76_RS03645 at 753.773 kb on - strand, within BWI76_RS03645 at 753.774 kb on - strand, within BWI76_RS03645 at 753.848 kb on - strand at 753.848 kb on - strand at 753.858 kb on - strand at 753.858 kb on - strand at 753.866 kb on - strand at 753.874 kb on - strand at 753.874 kb on - strand at 753.874 kb on - strand at 753.885 kb on + strand at 753.887 kb on - strand at 753.887 kb on - strand at 753.887 kb on - strand at 753.888 kb on - strand at 753.891 kb on + strand at 753.893 kb on - strand at 753.902 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.903 kb on + strand at 753.910 kb on - strand at 753.910 kb on - strand at 753.910 kb on - strand at 753.910 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.911 kb on - strand at 753.937 kb on + strand at 753.954 kb on - strand at 753.956 kb on - strand at 753.987 kb on + strand at 754.018 kb on - strand at 754.093 kb on - strand at 754.170 kb on + strand at 754.177 kb on + strand at 754.177 kb on + strand at 754.177 kb on + strand at 754.185 kb on + strand at 754.198 kb on + strand at 754.198 kb on + strand at 754.203 kb on + strand at 754.211 kb on + strand at 754.219 kb on - strand at 754.219 kb on - strand at 754.243 kb on + strand at 754.243 kb on + strand at 754.258 kb on - strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.261 kb on + strand at 754.262 kb on + strand at 754.262 kb on + strand at 754.262 kb on - strand at 754.269 kb on - strand at 754.269 kb on - strand at 754.269 kb on - strand at 754.269 kb on - strand at 754.269 kb on - strand at 754.270 kb on - strand at 754.276 kb on - strand at 754.288 kb on + strand at 754.303 kb on + strand at 754.313 kb on + strand at 754.320 kb on - strand at 754.320 kb on - strand at 754.320 kb on - strand at 754.333 kb on + strand at 754.351 kb on + strand at 754.351 kb on + strand at 754.351 kb on + strand at 754.351 kb on + strand at 754.354 kb on - strand at 754.354 kb on - strand at 754.356 kb on + strand at 754.359 kb on - strand at 754.359 kb on - strand at 754.359 kb on - strand at 754.361 kb on + strand at 754.361 kb on + strand at 754.361 kb on + strand at 754.361 kb on + strand at 754.364 kb on - strand at 754.364 kb on - strand at 754.392 kb on - strand at 754.394 kb on + strand at 754.394 kb on + strand at 754.400 kb on - strand at 754.402 kb on - strand at 754.402 kb on - strand at 754.402 kb on - strand at 754.402 kb on - strand at 754.407 kb on - strand at 754.422 kb on + strand, within BWI76_RS03650 at 754.422 kb on + strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.430 kb on - strand, within BWI76_RS03650 at 754.439 kb on + strand, within BWI76_RS03650 at 754.439 kb on + strand, within BWI76_RS03650 at 754.488 kb on - strand, within BWI76_RS03650 at 754.488 kb on - strand, within BWI76_RS03650 at 754.498 kb on - strand, within BWI76_RS03650 at 754.520 kb on + strand, within BWI76_RS03650 at 754.520 kb on + strand, within BWI76_RS03650 at 754.520 kb on + strand, within BWI76_RS03650 at 754.523 kb on - strand, within BWI76_RS03650 at 754.523 kb on - strand, within BWI76_RS03650 at 754.523 kb on - strand, within BWI76_RS03650 at 754.527 kb on + strand, within BWI76_RS03650 at 754.528 kb on - strand, within BWI76_RS03650 at 754.528 kb on - strand, within BWI76_RS03650 at 754.528 kb on - strand, within BWI76_RS03650 at 754.535 kb on - strand, within BWI76_RS03650 at 754.610 kb on + strand, within BWI76_RS03650 at 754.632 kb on - strand, within BWI76_RS03650 at 754.632 kb on - strand, within BWI76_RS03650 at 754.649 kb on - strand, within BWI76_RS03650 at 754.655 kb on - strand, within BWI76_RS03650 at 754.679 kb on + strand, within BWI76_RS03650 at 754.679 kb on + strand, within BWI76_RS03650 at 754.679 kb on + strand, within BWI76_RS03650 at 754.687 kb on - strand, within BWI76_RS03650 at 754.687 kb on - strand, within BWI76_RS03650 at 754.687 kb on - strand, within BWI76_RS03650 at 754.687 kb on - strand, within BWI76_RS03650 at 754.715 kb on - strand at 754.717 kb on + strand at 754.725 kb on - strand at 754.733 kb on - strand at 754.755 kb on - strand, within BWI76_RS03655 at 754.755 kb on - strand, within BWI76_RS03655 at 754.768 kb on + strand, within BWI76_RS03655 at 754.769 kb on + strand, within BWI76_RS03655 at 754.770 kb on - strand, within BWI76_RS03655 at 754.776 kb on - strand, within BWI76_RS03655 at 754.810 kb on - strand, within BWI76_RS03655 at 754.861 kb on + strand, within BWI76_RS03655 at 754.874 kb on - strand, within BWI76_RS03655 at 754.876 kb on - strand, within BWI76_RS03655 at 754.877 kb on + strand, within BWI76_RS03655 at 754.948 kb on + strand at 755.084 kb on - strand, within BWI76_RS03660 at 755.185 kb on + strand, within BWI76_RS03660 at 755.435 kb on - strand, within BWI76_RS03660
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 753,580 + BWI76_RS03645 0.61 -1.4 753,673 - BWI76_RS03645 0.71 +0.1 753,684 - BWI76_RS03645 0.72 -3.3 753,730 + BWI76_RS03645 0.78 +0.4 753,744 + BWI76_RS03645 0.79 +0.5 753,773 - BWI76_RS03645 0.82 -0.3 753,774 - BWI76_RS03645 0.82 -0.5 753,848 - -0.5 753,848 - +0.1 753,858 - -0.2 753,858 - -0.4 753,866 - +0.4 753,874 - +2.0 753,874 - -0.3 753,874 - +0.2 753,885 + +1.0 753,887 - +1.0 753,887 - +0.3 753,887 - +0.2 753,888 - -0.4 753,891 + +0.7 753,893 - +1.0 753,902 + +1.0 753,903 + +0.9 753,903 + +0.4 753,903 + -0.9 753,903 + -0.6 753,903 + +1.0 753,903 + -0.0 753,910 - -0.2 753,910 - +0.4 753,910 - -2.3 753,910 - +0.3 753,911 - -0.8 753,911 - -0.1 753,911 - +1.6 753,911 - +1.7 753,911 - -1.8 753,911 - +0.6 753,911 - -0.2 753,911 - +0.9 753,911 - -0.2 753,937 + -1.3 753,954 - +0.4 753,956 - -0.2 753,987 + -0.8 754,018 - -1.0 754,093 - -1.0 754,170 + +0.6 754,177 + -0.4 754,177 + +0.6 754,177 + -0.1 754,185 + +0.5 754,198 + -0.1 754,198 + +0.2 754,203 + +0.0 754,211 + +0.1 754,219 - -1.6 754,219 - -2.3 754,243 + +0.7 754,243 + -0.0 754,258 - +1.2 754,261 + +0.3 754,261 + -0.6 754,261 + -0.9 754,261 + -0.1 754,261 + -0.6 754,261 + -0.4 754,261 + -0.6 754,261 + -0.4 754,261 + +0.2 754,261 + -0.4 754,261 + +1.0 754,261 + +1.3 754,261 + +0.1 754,261 + -0.1 754,261 + +0.3 754,261 + -0.5 754,261 + +1.0 754,262 + -0.0 754,262 + +0.3 754,262 - +1.0 754,269 - +0.2 754,269 - +0.9 754,269 - +1.0 754,269 - -0.3 754,269 - +1.4 754,270 - +0.2 754,276 - -0.0 754,288 + +1.0 754,303 + +1.3 754,313 + +0.0 754,320 - -0.8 754,320 - -1.0 754,320 - -0.0 754,333 + -0.3 754,351 + -1.0 754,351 + +0.0 754,351 + +1.6 754,351 + +0.3 754,354 - -0.8 754,354 - -0.5 754,356 + +0.5 754,359 - +1.3 754,359 - +0.0 754,359 - -1.0 754,361 + +0.3 754,361 + +0.4 754,361 + -0.4 754,361 + -0.3 754,364 - -0.5 754,364 - -0.5 754,392 - +1.6 754,394 + +0.2 754,394 + +0.3 754,400 - +1.1 754,402 - +0.7 754,402 - -0.1 754,402 - +0.3 754,402 - +0.6 754,407 - +1.0 754,422 + BWI76_RS03650 0.11 -0.5 754,422 + BWI76_RS03650 0.11 -0.9 754,430 - BWI76_RS03650 0.13 +0.4 754,430 - BWI76_RS03650 0.13 -0.0 754,430 - BWI76_RS03650 0.13 +1.0 754,430 - BWI76_RS03650 0.13 -0.1 754,430 - BWI76_RS03650 0.13 -0.9 754,439 + BWI76_RS03650 0.16 -0.0 754,439 + BWI76_RS03650 0.16 -1.1 754,488 - BWI76_RS03650 0.30 -1.7 754,488 - BWI76_RS03650 0.30 +1.0 754,498 - BWI76_RS03650 0.33 -0.9 754,520 + BWI76_RS03650 0.40 -0.2 754,520 + BWI76_RS03650 0.40 -0.5 754,520 + BWI76_RS03650 0.40 +0.2 754,523 - BWI76_RS03650 0.41 -0.8 754,523 - BWI76_RS03650 0.41 -0.6 754,523 - BWI76_RS03650 0.41 -0.6 754,527 + BWI76_RS03650 0.42 +0.2 754,528 - BWI76_RS03650 0.42 +0.1 754,528 - BWI76_RS03650 0.42 -0.3 754,528 - BWI76_RS03650 0.42 +1.6 754,535 - BWI76_RS03650 0.44 -0.6 754,610 + BWI76_RS03650 0.67 -2.6 754,632 - BWI76_RS03650 0.73 -1.0 754,632 - BWI76_RS03650 0.73 -1.1 754,649 - BWI76_RS03650 0.78 +0.0 754,655 - BWI76_RS03650 0.80 +1.2 754,679 + BWI76_RS03650 0.87 -0.4 754,679 + BWI76_RS03650 0.87 +0.0 754,679 + BWI76_RS03650 0.87 +0.4 754,687 - BWI76_RS03650 0.90 +1.2 754,687 - BWI76_RS03650 0.90 -1.2 754,687 - BWI76_RS03650 0.90 -0.4 754,687 - BWI76_RS03650 0.90 -0.9 754,715 - +1.4 754,717 + +0.4 754,725 - +0.2 754,733 - +0.0 754,755 - BWI76_RS03655 0.17 -1.1 754,755 - BWI76_RS03655 0.17 +1.0 754,768 + BWI76_RS03655 0.24 +2.0 754,769 + BWI76_RS03655 0.24 +0.5 754,770 - BWI76_RS03655 0.25 +0.6 754,776 - BWI76_RS03655 0.28 -0.3 754,810 - BWI76_RS03655 0.45 +1.0 754,861 + BWI76_RS03655 0.70 +0.2 754,874 - BWI76_RS03655 0.77 -1.1 754,876 - BWI76_RS03655 0.78 -0.7 754,877 + BWI76_RS03655 0.78 -1.0 754,948 + -0.1 755,084 - BWI76_RS03660 0.10 -0.6 755,185 + BWI76_RS03660 0.16 +0.4 755,435 - BWI76_RS03660 0.29 -1.3
Or see this region's nucleotide sequence