Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23015 and BWI76_RS23020 are separated by 8 nucleotides BWI76_RS23020 and BWI76_RS23025 overlap by 14 nucleotides BWI76_RS23025 and BWI76_RS23030 are separated by 1 nucleotides BWI76_RS23030 and BWI76_RS23035 overlap by 4 nucleotides BWI76_RS23035 and BWI76_RS23040 overlap by 4 nucleotides
BWI76_RS23015: BWI76_RS23015 - cobalt transporter ATP-binding subunit, at 4,802,035 to 4,802,850
_RS23015
BWI76_RS23020: BWI76_RS23020 - cobalamin biosynthesis protein CbiQ, at 4,802,859 to 4,803,536
_RS23020
BWI76_RS23025: BWI76_RS23025 - cobalt ABC transporter substrate-binding protein CbiN, at 4,803,523 to 4,803,804
_RS23025
BWI76_RS23030: BWI76_RS23030 - cobalamin biosynthesis protein CbiM, at 4,803,806 to 4,804,543
_RS23030
BWI76_RS23035: BWI76_RS23035 - precorrin-2 C(20)-methyltransferase, at 4,804,540 to 4,805,253
_RS23035
BWI76_RS23040: BWI76_RS23040 - sirohydrochlorin cobaltochelatase, at 4,805,250 to 4,806,044
_RS23040
Position (kb) 4803
4804
4805 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4802.858 kb on - strand at 4802.875 kb on + strand at 4802.875 kb on + strand at 4803.106 kb on + strand, within BWI76_RS23020 at 4803.114 kb on - strand, within BWI76_RS23020 at 4803.392 kb on - strand, within BWI76_RS23020 at 4803.606 kb on - strand, within BWI76_RS23025 at 4803.768 kb on - strand, within BWI76_RS23025 at 4804.086 kb on - strand, within BWI76_RS23030 at 4804.305 kb on + strand, within BWI76_RS23030 at 4804.429 kb on + strand at 4804.681 kb on - strand, within BWI76_RS23035 at 4804.823 kb on + strand, within BWI76_RS23035 at 4804.904 kb on - strand, within BWI76_RS23035 at 4804.994 kb on + strand, within BWI76_RS23035 at 4805.015 kb on + strand, within BWI76_RS23035 at 4805.376 kb on - strand, within BWI76_RS23040 at 4805.517 kb on + strand, within BWI76_RS23040
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 4,802,858 - +0.3 4,802,875 + -0.1 4,802,875 + -0.7 4,803,106 + BWI76_RS23020 0.36 +2.1 4,803,114 - BWI76_RS23020 0.38 -2.1 4,803,392 - BWI76_RS23020 0.79 +0.6 4,803,606 - BWI76_RS23025 0.29 +0.9 4,803,768 - BWI76_RS23025 0.87 +0.8 4,804,086 - BWI76_RS23030 0.38 +1.2 4,804,305 + BWI76_RS23030 0.68 +0.5 4,804,429 + +0.9 4,804,681 - BWI76_RS23035 0.20 -0.1 4,804,823 + BWI76_RS23035 0.40 -0.1 4,804,904 - BWI76_RS23035 0.51 -0.5 4,804,994 + BWI76_RS23035 0.64 -0.6 4,805,015 + BWI76_RS23035 0.67 -1.0 4,805,376 - BWI76_RS23040 0.16 +1.2 4,805,517 + BWI76_RS23040 0.34 -0.0
Or see this region's nucleotide sequence