Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22010 and BWI76_RS22015 are separated by 474 nucleotides BWI76_RS22015 and BWI76_RS22020 are separated by 4 nucleotides BWI76_RS22020 and BWI76_RS22025 are separated by 36 nucleotides BWI76_RS22025 and BWI76_RS22030 are separated by 149 nucleotides BWI76_RS22030 and BWI76_RS22035 are separated by 63 nucleotides
BWI76_RS22010: BWI76_RS22010 - DNA binding protein, nucleoid-associated, at 4,597,791 to 4,598,192
_RS22010
BWI76_RS22015: BWI76_RS22015 - hypothetical protein, at 4,598,667 to 4,598,894
_RS22015
BWI76_RS22020: BWI76_RS22020 - L-alanine exporter AlaE, at 4,598,899 to 4,599,354
_RS22020
BWI76_RS22025: BWI76_RS22025 - hypothetical protein, at 4,599,391 to 4,599,741
_RS22025
BWI76_RS22030: BWI76_RS22030 - membrane protein, at 4,599,891 to 4,600,217
_RS22030
BWI76_RS22035: BWI76_RS22035 - DeoR family transcriptional regulator, at 4,600,281 to 4,601,639
_RS22035
Position (kb) 4598
4599
4600 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4597.920 kb on + strand, within BWI76_RS22010 at 4597.951 kb on + strand, within BWI76_RS22010 at 4597.951 kb on + strand, within BWI76_RS22010 at 4597.951 kb on + strand, within BWI76_RS22010 at 4597.959 kb on - strand, within BWI76_RS22010 at 4597.959 kb on - strand, within BWI76_RS22010 at 4597.959 kb on - strand, within BWI76_RS22010 at 4597.964 kb on - strand, within BWI76_RS22010 at 4597.978 kb on + strand, within BWI76_RS22010 at 4597.978 kb on + strand, within BWI76_RS22010 at 4597.978 kb on + strand, within BWI76_RS22010 at 4597.979 kb on + strand, within BWI76_RS22010 at 4597.981 kb on - strand, within BWI76_RS22010 at 4597.981 kb on - strand, within BWI76_RS22010 at 4597.987 kb on - strand, within BWI76_RS22010 at 4598.025 kb on + strand, within BWI76_RS22010 at 4598.041 kb on - strand, within BWI76_RS22010 at 4598.045 kb on + strand, within BWI76_RS22010 at 4598.045 kb on + strand, within BWI76_RS22010 at 4598.045 kb on + strand, within BWI76_RS22010 at 4598.045 kb on + strand, within BWI76_RS22010 at 4598.054 kb on - strand, within BWI76_RS22010 at 4598.054 kb on - strand, within BWI76_RS22010 at 4598.054 kb on - strand, within BWI76_RS22010 at 4598.054 kb on - strand, within BWI76_RS22010 at 4598.064 kb on - strand, within BWI76_RS22010 at 4598.071 kb on - strand, within BWI76_RS22010 at 4598.089 kb on + strand, within BWI76_RS22010 at 4598.089 kb on + strand, within BWI76_RS22010 at 4598.091 kb on - strand, within BWI76_RS22010 at 4598.095 kb on + strand, within BWI76_RS22010 at 4598.095 kb on + strand, within BWI76_RS22010 at 4598.096 kb on + strand, within BWI76_RS22010 at 4598.097 kb on - strand, within BWI76_RS22010 at 4598.097 kb on - strand, within BWI76_RS22010 at 4598.097 kb on - strand, within BWI76_RS22010 at 4598.097 kb on - strand, within BWI76_RS22010 at 4598.103 kb on - strand, within BWI76_RS22010 at 4598.105 kb on + strand, within BWI76_RS22010 at 4598.105 kb on + strand, within BWI76_RS22010 at 4598.128 kb on - strand, within BWI76_RS22010 at 4598.128 kb on - strand, within BWI76_RS22010 at 4598.128 kb on - strand, within BWI76_RS22010 at 4598.128 kb on - strand, within BWI76_RS22010 at 4598.128 kb on - strand at 4598.250 kb on - strand at 4598.365 kb on - strand at 4598.365 kb on - strand at 4598.391 kb on + strand at 4598.391 kb on + strand at 4598.419 kb on + strand at 4598.428 kb on - strand at 4598.428 kb on - strand at 4598.428 kb on - strand at 4598.434 kb on + strand at 4598.439 kb on + strand at 4598.444 kb on + strand at 4598.444 kb on + strand at 4598.444 kb on + strand at 4598.452 kb on - strand at 4598.454 kb on + strand at 4598.459 kb on + strand at 4598.462 kb on - strand at 4598.472 kb on - strand at 4598.472 kb on - strand at 4598.593 kb on + strand at 4598.596 kb on - strand at 4598.596 kb on - strand at 4598.730 kb on + strand, within BWI76_RS22015 at 4598.742 kb on + strand, within BWI76_RS22015 at 4598.750 kb on - strand, within BWI76_RS22015 at 4598.820 kb on + strand, within BWI76_RS22015 at 4598.820 kb on + strand, within BWI76_RS22015 at 4598.828 kb on - strand, within BWI76_RS22015 at 4598.828 kb on - strand, within BWI76_RS22015 at 4598.828 kb on - strand, within BWI76_RS22015 at 4598.889 kb on - strand at 4598.903 kb on + strand at 4598.903 kb on + strand at 4598.903 kb on + strand at 4598.903 kb on + strand at 4598.908 kb on + strand at 4598.908 kb on + strand at 4598.911 kb on - strand at 4598.916 kb on - strand at 4598.935 kb on + strand at 4598.942 kb on + strand at 4598.950 kb on - strand, within BWI76_RS22020 at 4598.950 kb on - strand, within BWI76_RS22020 at 4598.976 kb on - strand, within BWI76_RS22020 at 4599.130 kb on - strand, within BWI76_RS22020 at 4599.218 kb on + strand, within BWI76_RS22020 at 4599.302 kb on + strand at 4599.337 kb on + strand at 4599.346 kb on - strand at 4599.351 kb on - strand at 4599.383 kb on - strand at 4599.402 kb on - strand at 4599.458 kb on - strand, within BWI76_RS22025 at 4599.470 kb on + strand, within BWI76_RS22025 at 4599.470 kb on + strand, within BWI76_RS22025 at 4599.470 kb on + strand, within BWI76_RS22025 at 4599.470 kb on + strand, within BWI76_RS22025 at 4599.478 kb on - strand, within BWI76_RS22025 at 4599.478 kb on - strand, within BWI76_RS22025 at 4599.552 kb on + strand, within BWI76_RS22025 at 4599.558 kb on + strand, within BWI76_RS22025 at 4599.558 kb on + strand, within BWI76_RS22025 at 4599.560 kb on - strand, within BWI76_RS22025 at 4599.563 kb on + strand, within BWI76_RS22025 at 4599.563 kb on + strand, within BWI76_RS22025 at 4599.566 kb on - strand, within BWI76_RS22025 at 4599.571 kb on - strand, within BWI76_RS22025 at 4599.571 kb on - strand, within BWI76_RS22025 at 4599.581 kb on - strand, within BWI76_RS22025 at 4599.607 kb on + strand, within BWI76_RS22025 at 4599.607 kb on + strand, within BWI76_RS22025 at 4599.607 kb on + strand, within BWI76_RS22025 at 4599.607 kb on + strand, within BWI76_RS22025 at 4599.614 kb on - strand, within BWI76_RS22025 at 4599.615 kb on - strand, within BWI76_RS22025 at 4599.615 kb on - strand, within BWI76_RS22025 at 4599.615 kb on - strand, within BWI76_RS22025 at 4599.618 kb on - strand, within BWI76_RS22025 at 4599.651 kb on + strand, within BWI76_RS22025 at 4599.652 kb on + strand, within BWI76_RS22025 at 4599.658 kb on + strand, within BWI76_RS22025 at 4599.659 kb on - strand, within BWI76_RS22025 at 4599.659 kb on - strand, within BWI76_RS22025 at 4599.660 kb on - strand, within BWI76_RS22025 at 4599.660 kb on - strand, within BWI76_RS22025 at 4599.661 kb on - strand, within BWI76_RS22025 at 4599.666 kb on - strand, within BWI76_RS22025 at 4599.681 kb on + strand, within BWI76_RS22025 at 4599.767 kb on + strand at 4599.768 kb on + strand at 4599.768 kb on + strand at 4599.769 kb on + strand at 4599.775 kb on - strand at 4599.776 kb on - strand at 4599.776 kb on - strand at 4599.776 kb on - strand at 4599.776 kb on - strand at 4599.779 kb on + strand at 4599.781 kb on - strand at 4599.781 kb on - strand at 4599.782 kb on - strand at 4599.782 kb on - strand at 4599.823 kb on + strand at 4599.831 kb on - strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on - strand at 4599.858 kb on - strand at 4599.866 kb on - strand at 4599.866 kb on - strand at 4599.866 kb on - strand at 4599.866 kb on - strand at 4599.866 kb on - strand at 4599.866 kb on - strand at 4599.866 kb on - strand at 4599.874 kb on + strand at 4599.906 kb on + strand at 4599.906 kb on + strand at 4599.914 kb on - strand at 4599.914 kb on - strand at 4599.914 kb on - strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.918 kb on + strand at 4599.918 kb on + strand at 4599.918 kb on + strand at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.926 kb on - strand, within BWI76_RS22030 at 4599.928 kb on + strand, within BWI76_RS22030 at 4599.970 kb on + strand, within BWI76_RS22030 at 4599.978 kb on - strand, within BWI76_RS22030 at 4600.024 kb on - strand, within BWI76_RS22030 at 4600.064 kb on + strand, within BWI76_RS22030 at 4600.078 kb on - strand, within BWI76_RS22030 at 4600.110 kb on + strand, within BWI76_RS22030 at 4600.120 kb on + strand, within BWI76_RS22030 at 4600.123 kb on - strand, within BWI76_RS22030 at 4600.266 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 4,597,920 + BWI76_RS22010 0.32 -1.9 4,597,951 + BWI76_RS22010 0.40 -1.1 4,597,951 + BWI76_RS22010 0.40 -0.6 4,597,951 + BWI76_RS22010 0.40 -0.1 4,597,959 - BWI76_RS22010 0.42 +0.8 4,597,959 - BWI76_RS22010 0.42 +0.9 4,597,959 - BWI76_RS22010 0.42 +0.6 4,597,964 - BWI76_RS22010 0.43 -0.9 4,597,978 + BWI76_RS22010 0.47 -1.0 4,597,978 + BWI76_RS22010 0.47 -2.0 4,597,978 + BWI76_RS22010 0.47 -0.1 4,597,979 + BWI76_RS22010 0.47 -1.4 4,597,981 - BWI76_RS22010 0.47 +0.0 4,597,981 - BWI76_RS22010 0.47 +0.5 4,597,987 - BWI76_RS22010 0.49 -0.5 4,598,025 + BWI76_RS22010 0.58 -0.1 4,598,041 - BWI76_RS22010 0.62 -0.2 4,598,045 + BWI76_RS22010 0.63 +0.9 4,598,045 + BWI76_RS22010 0.63 +0.9 4,598,045 + BWI76_RS22010 0.63 -1.7 4,598,045 + BWI76_RS22010 0.63 -3.4 4,598,054 - BWI76_RS22010 0.65 +0.9 4,598,054 - BWI76_RS22010 0.65 -0.6 4,598,054 - BWI76_RS22010 0.65 -1.4 4,598,054 - BWI76_RS22010 0.65 +0.4 4,598,064 - BWI76_RS22010 0.68 +1.9 4,598,071 - BWI76_RS22010 0.70 -1.4 4,598,089 + BWI76_RS22010 0.74 +0.7 4,598,089 + BWI76_RS22010 0.74 -1.1 4,598,091 - BWI76_RS22010 0.75 -0.6 4,598,095 + BWI76_RS22010 0.76 +1.9 4,598,095 + BWI76_RS22010 0.76 +0.9 4,598,096 + BWI76_RS22010 0.76 -1.1 4,598,097 - BWI76_RS22010 0.76 -0.4 4,598,097 - BWI76_RS22010 0.76 +0.8 4,598,097 - BWI76_RS22010 0.76 +0.9 4,598,097 - BWI76_RS22010 0.76 -1.0 4,598,103 - BWI76_RS22010 0.78 -0.1 4,598,105 + BWI76_RS22010 0.78 +0.3 4,598,105 + BWI76_RS22010 0.78 -0.8 4,598,128 - BWI76_RS22010 0.84 -3.5 4,598,128 - BWI76_RS22010 0.84 -2.6 4,598,128 - BWI76_RS22010 0.84 +0.5 4,598,128 - BWI76_RS22010 0.84 -1.9 4,598,128 - +0.9 4,598,250 - -1.9 4,598,365 - -0.1 4,598,365 - -1.6 4,598,391 + +0.6 4,598,391 + -0.1 4,598,419 + +0.5 4,598,428 - -0.6 4,598,428 - -1.8 4,598,428 - -0.2 4,598,434 + +0.9 4,598,439 + -2.2 4,598,444 + -0.5 4,598,444 + +0.1 4,598,444 + -1.6 4,598,452 - -1.9 4,598,454 + -1.6 4,598,459 + -2.0 4,598,462 - -0.6 4,598,472 - +0.6 4,598,472 - -1.8 4,598,593 + +0.7 4,598,596 - +0.7 4,598,596 - +0.4 4,598,730 + BWI76_RS22015 0.28 -0.9 4,598,742 + BWI76_RS22015 0.33 +0.1 4,598,750 - BWI76_RS22015 0.36 -1.8 4,598,820 + BWI76_RS22015 0.67 -0.6 4,598,820 + BWI76_RS22015 0.67 +2.4 4,598,828 - BWI76_RS22015 0.71 -1.0 4,598,828 - BWI76_RS22015 0.71 +0.2 4,598,828 - BWI76_RS22015 0.71 -1.4 4,598,889 - +0.2 4,598,903 + -1.1 4,598,903 + +1.1 4,598,903 + -0.6 4,598,903 + -0.6 4,598,908 + -0.2 4,598,908 + +1.5 4,598,911 - -0.6 4,598,916 - +0.4 4,598,935 + -1.5 4,598,942 + -0.1 4,598,950 - BWI76_RS22020 0.11 -1.6 4,598,950 - BWI76_RS22020 0.11 -2.1 4,598,976 - BWI76_RS22020 0.17 -1.3 4,599,130 - BWI76_RS22020 0.51 -0.8 4,599,218 + BWI76_RS22020 0.70 -1.6 4,599,302 + +3.3 4,599,337 + -0.2 4,599,346 - -0.2 4,599,351 - -0.8 4,599,383 - +0.3 4,599,402 - -0.2 4,599,458 - BWI76_RS22025 0.19 -1.9 4,599,470 + BWI76_RS22025 0.23 +0.8 4,599,470 + BWI76_RS22025 0.23 -0.1 4,599,470 + BWI76_RS22025 0.23 -1.5 4,599,470 + BWI76_RS22025 0.23 +1.6 4,599,478 - BWI76_RS22025 0.25 -4.2 4,599,478 - BWI76_RS22025 0.25 +0.4 4,599,552 + BWI76_RS22025 0.46 +0.1 4,599,558 + BWI76_RS22025 0.48 +0.9 4,599,558 + BWI76_RS22025 0.48 -0.3 4,599,560 - BWI76_RS22025 0.48 -0.3 4,599,563 + BWI76_RS22025 0.49 +0.7 4,599,563 + BWI76_RS22025 0.49 -2.2 4,599,566 - BWI76_RS22025 0.50 +0.6 4,599,571 - BWI76_RS22025 0.51 +0.1 4,599,571 - BWI76_RS22025 0.51 -1.4 4,599,581 - BWI76_RS22025 0.54 -0.9 4,599,607 + BWI76_RS22025 0.62 -2.6 4,599,607 + BWI76_RS22025 0.62 +0.2 4,599,607 + BWI76_RS22025 0.62 -2.2 4,599,607 + BWI76_RS22025 0.62 -1.1 4,599,614 - BWI76_RS22025 0.64 -0.4 4,599,615 - BWI76_RS22025 0.64 +0.4 4,599,615 - BWI76_RS22025 0.64 +0.6 4,599,615 - BWI76_RS22025 0.64 -0.8 4,599,618 - BWI76_RS22025 0.65 -0.1 4,599,651 + BWI76_RS22025 0.74 -0.1 4,599,652 + BWI76_RS22025 0.74 -3.6 4,599,658 + BWI76_RS22025 0.76 -0.2 4,599,659 - BWI76_RS22025 0.76 +0.6 4,599,659 - BWI76_RS22025 0.76 +0.2 4,599,660 - BWI76_RS22025 0.77 -1.6 4,599,660 - BWI76_RS22025 0.77 -1.0 4,599,661 - BWI76_RS22025 0.77 -0.3 4,599,666 - BWI76_RS22025 0.78 -0.1 4,599,681 + BWI76_RS22025 0.83 -0.4 4,599,767 + -1.3 4,599,768 + +0.9 4,599,768 + +0.4 4,599,769 + -0.3 4,599,775 - +0.4 4,599,776 - -0.0 4,599,776 - -0.2 4,599,776 - +0.6 4,599,776 - -0.1 4,599,779 + -1.4 4,599,781 - -0.6 4,599,781 - -0.1 4,599,782 - +0.6 4,599,782 - +0.5 4,599,823 + +0.9 4,599,831 - +0.6 4,599,858 + -0.3 4,599,858 + -2.9 4,599,858 + +0.3 4,599,858 + +0.7 4,599,858 + -0.1 4,599,858 + -1.6 4,599,858 + -0.2 4,599,858 + -0.2 4,599,858 + +0.7 4,599,858 - +0.7 4,599,858 - -0.2 4,599,866 - +0.6 4,599,866 - -0.0 4,599,866 - -0.1 4,599,866 - -0.5 4,599,866 - +0.8 4,599,866 - +0.0 4,599,866 - -1.6 4,599,874 + +2.3 4,599,906 + -0.1 4,599,906 + +0.7 4,599,914 - -0.1 4,599,914 - -2.6 4,599,914 - -0.1 4,599,916 + +1.5 4,599,916 + -1.8 4,599,916 + -1.5 4,599,916 + +1.0 4,599,916 + +0.6 4,599,916 + +1.1 4,599,916 + +0.1 4,599,916 + -1.2 4,599,916 + +0.2 4,599,916 + -0.6 4,599,916 + -0.3 4,599,918 + +0.9 4,599,918 + -0.4 4,599,918 + -3.0 4,599,924 - BWI76_RS22030 0.10 -2.1 4,599,924 - BWI76_RS22030 0.10 +0.9 4,599,924 - BWI76_RS22030 0.10 +2.3 4,599,924 - BWI76_RS22030 0.10 -1.0 4,599,924 - BWI76_RS22030 0.10 +0.6 4,599,924 - BWI76_RS22030 0.10 -0.1 4,599,924 - BWI76_RS22030 0.10 +0.7 4,599,924 - BWI76_RS22030 0.10 +0.2 4,599,924 - BWI76_RS22030 0.10 -0.9 4,599,924 - BWI76_RS22030 0.10 +0.5 4,599,924 - BWI76_RS22030 0.10 -0.1 4,599,924 - BWI76_RS22030 0.10 -0.2 4,599,924 - BWI76_RS22030 0.10 +0.1 4,599,924 - BWI76_RS22030 0.10 -1.6 4,599,924 - BWI76_RS22030 0.10 +0.0 4,599,924 - BWI76_RS22030 0.10 +0.4 4,599,924 - BWI76_RS22030 0.10 -0.3 4,599,924 - BWI76_RS22030 0.10 -0.6 4,599,924 - BWI76_RS22030 0.10 +2.5 4,599,924 - BWI76_RS22030 0.10 -0.2 4,599,926 - BWI76_RS22030 0.11 -0.1 4,599,928 + BWI76_RS22030 0.11 -1.4 4,599,970 + BWI76_RS22030 0.24 +0.1 4,599,978 - BWI76_RS22030 0.27 -1.9 4,600,024 - BWI76_RS22030 0.41 +1.3 4,600,064 + BWI76_RS22030 0.53 +0.2 4,600,078 - BWI76_RS22030 0.57 -0.6 4,600,110 + BWI76_RS22030 0.67 +0.4 4,600,120 + BWI76_RS22030 0.70 -1.5 4,600,123 - BWI76_RS22030 0.71 -0.3 4,600,266 + -0.3
Or see this region's nucleotide sequence